Formation of apatite on poly(α‐hydroxy acid) in an accelerated biomimetic process

Chen, Yun; Mak, Arthur F. T.; Li, Jiashen; Wang, Min; Shum, Anita W.T.

doi:10.1002/jbm.b.30178

Cited by 67 publications

(76 citation statements)

References 36 publications

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“…17,[36][37][38][39] XRD results showed both coated scaffolds showed crystal peaks of mineral at 26°and 32°, which correspond to the (002) plane and (211) (112) planes of apatite. 40 Coated PLLA showed a peak at 32°, and other peaks due to the crystal structure of PLLA polymer, which were confirmed from the XRD data of uncoated PLLA scaffolds. 41 In contrast, coated PCL showed two peaks from the PCL polymer around 21°and 24°, similar to previous studies.…”

Section: L-ct For Nondestructive Characterization Of Biomineral Coatingssupporting

confidence: 63%

Use of Micro-Computed Tomography to Nondestructively Characterize Biomineral Coatings on Solid Freeform Fabricated Poly (L-Lactic Acid) and Poly (ɛ-Caprolactone) Scaffolds In Vitro and In Vivo

Saito

Suárez-González

Rao

et al. 2013

Tissue Engineering Part C: Methods

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Biomineral coatings have been extensively used to enhance the osteoconductivity of polymeric scaffolds. Numerous porous scaffolds have previously been coated with a bone-like apatite mineral through incubation in simulated body fluid (SBF). However, characterization of the mineral layer formed on scaffolds, including the amount of mineral within the scaffolds, often requires destructive methods. We have developed a method using micro-computed tomography (m-CT) scanning to nondestructively quantify the amount of mineral in vitro and in vivo on biodegradable scaffolds made of poly (L-lactic acid) (PLLA) and poly (e-caprolactone) (PCL). PLLA and PCL scaffolds were fabricated using an indirect solid freeform fabrication (SFF) technique to achieve orthogonally interconnected pore architectures. Biomineral coatings were formed on the fabricated PLLA and PCL scaffolds after incubation in modified SBF (mSBF). Scanning electron microscopy and X-ray diffraction confirmed the formation of an apatite-like mineral. The scaffolds were implanted into mouse ectopic sites for 3 and 10 weeks. The presence of a biomineral coating within the porous scaffolds was confirmed through plastic embedding and m-CT techniques. Tissue mineral content (TMC) and volume of mineral on the scaffold surfaces detected by m-CT had a strong correlation with the amount of calcium measured by the orthocresolphthalein complex-one (OCPC) method before and after implantation. There was a strong correlation between OCPC preand postimplantation and m-CT measured TMC (R 2 = 0.96 preimplant; R 2 = 0.90 postimplant) and mineral volume (R 2 = 0.96 preimplant; R 2 = 0.89 postimplant). The m-CT technique showed increases in mineral following implantation, suggesting that m-CT can be used to nondestructively determine the amount of calcium on coated scaffolds.

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Section: L-ct For Nondestructive Characterization Of Biomineral Coatingssupporting

confidence: 63%

Use of Micro-Computed Tomography to Nondestructively Characterize Biomineral Coatings on Solid Freeform Fabricated Poly (L-Lactic Acid) and Poly (ɛ-Caprolactone) Scaffolds In Vitro and In Vivo

Saito

Suárez-González

Rao

et al. 2013

Tissue Engineering Part C: Methods

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“…Therefore, uniformly coated porous PLGA scaffolds have been produced by using a system that is designed to induce SBF flow throughout the pores of the scaffold. 87 In parallel, PLGA scaffolds with apatite-coated pores have been developed with an alternative method to avoid immersion in SBF. This method involves inclusion of apatite-coated paraffin spheres into the PLGA followed by dissolution of the paraffin spheres.…”

Section: Scaffolds Consisting Of Pure Plgamentioning

confidence: 99%

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Lanao

Jonker

Wolke

et al. 2013

Tissue Engineering Part B: Reviews

175

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“…Seeing that the biomimetic coating would be helpful in coating biodegradable substrates such as Mg-alloys, it reduces the incubation times in SBF during the coating procedure [12]. As reported this resulted in the formation of non-uniform and porous Ca-P coating on Mg substrates [13], in addition to hydrogen bubbles formed on the Mg surface during immersion.…”

Section: Introductionmentioning

confidence: 94%

Effect of Adding Sb on Microstructure, Mechanical Properties and in <i>Vitro</i> Degradation Behavior of as Cast Mg-4wt% Zn Alloy for Medical Application

Mohamed¹,

Moussa²,

Waly³

et al. 2017

JSEMAT

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Magnesium (Mg) and its alloys are one of a novel kind of biodegradable metallic implants which attracted much fundamental research to develop its clinical application. Nevertheless, it has more restrictions in biomedical applications because it degrades too fast at the early stage after implantation, thus commonly leading to some problems such as early fast mechanical loss, hydric bubble aggregation, gap formation between the implants and the tissue. This work aims to study the effect of 0.5 wt% Sb addition on the microstructure, mechanical properties and degradation behavior of as cast Mg-4wt% Zn alloy. The evaluation process was conducted using optical and scanning electron microscopy, X-ray diffraction, tensile and compression tests, in addition to a corrosion study by immersing in simulated body fluid (SBF). Results showed that Sb refines the grain size of the base alloy and also enhances its mechanical properties and degradation rate as well. These were due to the formation of the secondary phase of Mg 3 Sb 2 . To get better degradation rate, the Mg-4wt% Zn and Mg-4wt% Zn-0.5wt% Sb alloys are coated with Ca-P using autocatalytic technique. The results demonstrated that the formed coat layer improves the degradation rate of samples under the condition of this study. The current study shows that Mg-4wt% Zn-0.5wt% Sb alloy has good mechanical properties and when it coated by Ca-P, it gave a better corrosion resistance that makes it ideal for biodegradable medical application.

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Formation of apatite on poly(α‐hydroxy acid) in an accelerated biomimetic process

Cited by 67 publications

References 36 publications

Use of Micro-Computed Tomography to Nondestructively Characterize Biomineral Coatings on Solid Freeform Fabricated Poly (L-Lactic Acid) and Poly (ɛ-Caprolactone) Scaffolds In Vitro and In Vivo

Use of Micro-Computed Tomography to Nondestructively Characterize Biomineral Coatings on Solid Freeform Fabricated Poly (L-Lactic Acid) and Poly (ɛ-Caprolactone) Scaffolds In Vitro and In Vivo

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Effect of Adding Sb on Microstructure, Mechanical Properties and in <i>Vitro</i> Degradation Behavior of as Cast Mg-4wt% Zn Alloy for Medical Application

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Formation of apatite on poly(α‐hydroxy acid) in an accelerated biomimetic process

Cited by 67 publications

References 36 publications

Use of Micro-Computed Tomography to Nondestructively Characterize Biomineral Coatings on Solid Freeform Fabricated Poly (L-Lactic Acid) and Poly (ɛ-Caprolactone) Scaffolds In Vitro and In Vivo

Use of Micro-Computed Tomography to Nondestructively Characterize Biomineral Coatings on Solid Freeform Fabricated Poly (L-Lactic Acid) and Poly (ɛ-Caprolactone) Scaffolds In Vitro and In Vivo

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Effect of Adding Sb on Microstructure, Mechanical Properties and in &lt;i&gt;Vitro&lt;/i&gt; Degradation Behavior of as Cast Mg-4wt% Zn Alloy for Medical Application

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Effect of Adding Sb on Microstructure, Mechanical Properties and in <i>Vitro</i> Degradation Behavior of as Cast Mg-4wt% Zn Alloy for Medical Application