Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Abstract

Background Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from Carassius auratus red var. (RCC, 2n = 100) (♀) and autotetraploid Carassius auratus (4nRR, 4n = 200) (♂). The female 3nRR produced haploid, diploid and triploid eggs, whereas the male 3nRR was infertile. The aim of the present study was to explore fertility of potential candidate genes of 3nRR. Results Gonadal transcriptome profiling of four groups (3 females RCC (FRCC), 3 males 4nRR (M4nRR), 3 males 3nRR (M3nRR) and 3 females 3nRR (F3nRR)) was performed using RNA-SEq. A total of 78.90 Gb of clean short reads and 24,262 differentially expressed transcripts (DETs), including 20,155 in F3nRR vs. FRCC and 4,107 in M3nRR vs. M4nRR were identified. A total of 106 enriched pathways were identified through KEGG enrichment analysis. Out of the enriched pathways, 44 and 62 signalling pathways were identified in F3nRR vs. FRCC and M3nRR vs. M4nRR, respectively. A total of 80 and 25 potential candidate genes for fertility-related in F3nRR and M3nRR were identified, respectively, through GO, KEGG analyses and the published literature. Moreover, protein-protein interaction (PPI) network construction of these fertility-associated genes were performed. Analysis of the PPI networks showed that 6 hub genes (MYC, SOX2, BMP4, GATA4, PTEN and BMP2) were involved in female fertility of F3nRR, and 2 hub genes (TP53 and FGF2) were involved in male sterility of M3nRR. Conclusions Establishment of autotriploid fish offers an ideal model to study reproductive traits of triploid fish. RNA-Seq data revealed 6 genes, namely, MYC, SOX2, BMP4, GATA4, PTEN and BMP2, involved in the female fertility of the F3nRR. Moreover, 2 genes, namely, TP53 and FGF2, were related to the male sterility of the M3nRR. These findings provide information on reproduction and breeding in triploid fish.

Reproductive performances of triploid male and female Nile tilapia Oreochromis niloticus (Linnaeus, 1758) at different ages

“…Since male germ cells do not undergo meiosis to sexual maturation, testicular development, in general, is not impaired in triploid males (Huang et al 2021;Xu et al 2023). Although triploid males show maturity sexually, it remains sterile because it produce aneuploid spermatozoa and do not produce life offspring, such as in common carp (Majhi et al 2017), tilapia (Pandit et al 2015), grass carp Ctenopharyngodon idella (Valenciennes, 1844) (Clemens et al 2016), and Carassius auratus (Linnaeus, 1758) (Wang et al 2021).…”

Identification and effective regulation of <i>scarb1</i> gene involved in pigmentation change in autotetraploid <i>Carassius auratus</i>

“…All samples were performed in triplicate. The specific RT-qPCR primers for each gene are listed in Supplementary Table S1, with β-actin and 18S selected as the optimal reference genes according to our previous study ( Du et al, 2021 ; Wang et al, 2021a ). The expression results were calculated using the 2 −ΔΔCt method ( Livak, 2001 ), with the expression levels of the housekeeping genes acquired using geometric mean expression of β-actin and 18S .…”

The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus

“…The identity and polypeptide sequence alignments of amino-acid sequences of Dmc1 were utilized with the DNAMAN version7.0. The qRT -PCR was carried out as directed by Wang et al (2021) . The qRT -PCR was conducted with triplicates.…”