Formation of Bones and Teeth as Visualized by Radioautography

Leblond, C. P.; Bélanger, Louise; Greulich, Richard C.

doi:10.1111/j.1749-6632.1955.tb40055.x

Cited by 87 publications

(14 citation statements)

References 17 publications

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“…1), indicating that these granules may represent secretory granules and ferry sulfated material into the extracellular matrix. This interpretation is consistent with previous light microscopic radioautographic results [12][13][14] demonstrating 35SO4 incorporation into osteoblasts and the extracellular bone matrix. However, the possibility remains that some of these cytoplasmic granules represent acid phosphatase positive phagolysosomes, which may endocytose extracellular sulfated material.…”

Section: Discussionsupporting

confidence: 93%

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

et al. 1983

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Glycosaminoglycans (GAGs) and glycoproteins are essential components for osteogenesis. We have examined rat osteoblasts, osteoid, transitional zone, and fully calcified bone matrix, utilizing Spicer's high-iron diaminethiocarbohydrazide-silver protein (HID-TCH-SP) method for sulfated glycoconjugates and Thiéry's periodate-TCH-SP (PA-TCH-SP) method for vicinal glycol-containing glycoconjugates. HID-TCH-SP stained cytoplasmic granules of osteoblasts. Stain deposits in the extracellular matrix were observed in decreasing amounts in osteoid, the transitional zone, and fully calcified bone matrix. Enzyme digestion with testicular hyaluronidase removed most HID-TCH-SP stain deposits. PA-TCH-SP staining was observed with increasing intensity in rough endoplasmic reticulum, Golgi saccules, and cytoplasmic granules. Collagen fibrils in osteoid were weakly stained with PA-TCH-SP, and their staining appeared even weaker in fully calcified bone matrix. In contrast, collagen fibrils in calcified cartilage stained intensely with the PA-TCH-SP method. Focal circular profiles (0.1-0.5 mumol in diameter), which lacked collagen fibrils but reacted moderately with PA-TCH-SP, were frequently seen in the transitional zone and fully calcified bone matrix, but were only occasionally present in osteoid. The presence of testicular hyaluronidase-resistant GAG and acid phosphatase in these focal areas suggests that they represent sites of GAG degradation. The eventual loss of HID-TCH-SP staining in the bone matrix suggests that removal of sulfated glycoconjugates may be a requisite for expansion of initial calcification sites and/or complete calcification.

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Section: Discussionsupporting

confidence: 93%

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

et al. 1983

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“…Keratan sulphate moderately stained the odontoblastic layer The studies reported by Takagi et al (1990) confirm the heterogeneous distribution of proteoglycan in rat predentin and dentin, a feature that is evident in most tissues, and is a common feature of proteoglycan biology. The observation of proteoglycan staining in the odontoblastic layer is consistent with previous autoradiographic studies (Leblond et al, 1955;Sundstrom, 1971;Goldberg and Escaig, 1985), demonstrating 35 S sulphate incorporation into odontoblasts, predentin, and dentin; however, these studies could not identify glycosaminoglycan types. Evidence that the majority of the demonstrable glycosaminoglycan in predentin is chondroitin 4 sulphate is consistent with biochemical studies of rats and other species (Engfeldt and Hjerpe, 1972;Prince et al, 1984;Rahemtulla et al, 1984), and also demonstrates a significant decrease in the amount of glycosaminoglycan in dentin.…”

Section: Predentin-dentinsupporting

confidence: 91%

The Use of Immunohistochemistry in Understanding the Structure and Function of the Extracellular Matrix of Dental Tissues

Hall

Embery

1997

Adv Dent Res.

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The availability of monoclonal and polyclonal antibodies directed toward the recognition of epitopes in a variety of extracellular matrix components of the dentition represents a powerful tool in the investigation of the structure and biology of dental tissues in health and disease. The immunolocalization of both whole molecule structures and specific regions of molecules has the potential to yield information on tooth development, the effects of aging, changes in tooth structure during the initiation and progression of the caries process, together with the response of the tooth to restorative treatment. This review reports on current research to elucidate the role of extracellular matrices of enamel, dentin, cementum, and bone. Attention is directed at the use of antibodies toward the small leucine-rich proteoglycans such as decorin and biglycan, in addition to their glycosaminoglycan chains. Antibodies are also being developed toward dental tissue-specific macromolecules such as phosphophoryn and amelogenin; the use of these antibodies will increase our understanding of the role of these macromolecules in mineralized tissues.

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“…Preliminary experiments in mice killed 1 and 2 days after gold thioglucose injection also appear to support this notion, since at those early inter- vals radioactivity appeared diffusely distributed in the trabeculae with few hot spots. Similar initial diffuse labeling of bone which disappeared within 4 days was described by Leblond et al 9 following injection of S35-sulfate, though growth bands persisted as in the present observations. Work now in progress is directed to elucidate in greater detail the genesis of the exact localization of gold in joints.…”

Section: Discussionsupporting

confidence: 92%

The autoradiographic localization and distribution of neutron activated gold (Au¹⁹⁸) in skeletal tissues and synovia of mice

Tonna

Brecher

Cronkite

et al. 1963

Arthritis & Rheumatism

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The localization and distribution of neutron activated radioactive gold in the long bones and knee joints of mice was determined autoradiographically following treatment of animals with gold thioglucose and thiomalate. Gold was found 1 or 2 months later at the surface of the synovium, the osteogenic layer of the perichondrial periosteum, reticuloendothelial cells of the marrow and osteogenic cells along the surface of the metaphyseal trabeculae and secondary centers of ossification. Gold was not seen in cartilage.Le localisation e le distribution de neutrono-activate auro radioactive in le ossos longe e le articulationes geniculari de muses esseva determinate autoradiographicamente post le tractamento del animales con thioglucosa e thiomalato de auro. Auro esseva trovate 1 o 2 menses plus tarde a1 superficie del synovio, le strato osteogenic del periosteo perichondrial, le cellulas retidoendothelial del medulla, e le cellulas osteogenic a1 longo del superficie del trabeculas metaphyseal, e in centros secundari de ossification. Nulle auro esseva trovate in cartilagine.HE USEFULNESS of chrysotherapy in rheumatoid arthritis has been T demonstratedl and detailed biochemical studies of the absorption, excretion, and retention of gold compounds in experimental animals are available." Yet knowledge of the localization of these compounds within joints is quite limited. Bertrand et a1.t using radioactive gold ( AulQ8), demonstrated that its concentration was similar in bone, synovium and skin when rats were injected with A U~~* sodium thiosulfate, but that the gold concentration was significantly increased in synovium and bone when chemical arthritis had been produced by intra-articular injections of turpentine. Increased deposition of gold in arthritic joints of man has also been r e p~r t e d .~ The localization within the tissues, however, is unknown. Combination of activation analysis and autoradiography now offers the means for such exact localization. Ordinary gold ( Aule7) compounds may be injected and the tissues fixed at any desired

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Formation of Bones and Teeth as Visualized by Radioautography

Cited by 87 publications

References 17 publications

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

The Use of Immunohistochemistry in Understanding the Structure and Function of the Extracellular Matrix of Dental Tissues

The autoradiographic localization and distribution of neutron activated gold (Au¹⁹⁸) in skeletal tissues and synovia of mice

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Formation of Bones and Teeth as Visualized by Radioautography

Cited by 87 publications

References 17 publications

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

The Use of Immunohistochemistry in Understanding the Structure and Function of the Extracellular Matrix of Dental Tissues

The autoradiographic localization and distribution of neutron activated gold (Au198) in skeletal tissues and synovia of mice

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The autoradiographic localization and distribution of neutron activated gold (Au¹⁹⁸) in skeletal tissues and synovia of mice