Formation of cashew gum thin films onto silicon wafers or amino-terminated surfaces and the immobilization of Concanavalin A on them

Maciel, Jeanny da Silva; Kosaka, Priscila M.; Paula, Regina C.M. de; Feitosa, Judith P.A.; Petri, Denise Freitas Siqueira

doi:10.1016/j.carbpol.2007.01.013

Cited by 18 publications

(12 citation statements)

References 35 publications

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“…2a), for scan areas of 3 m × 3 m and 2.5 m × 2.5 m, respectively. Similar morphological features have been observed for ConA adsorbed onto carboxymethyl cellulose (CMC) [4,5] or cashew gum [10] films. Mean thickness values determined for ConA films when the polysaccharide surface was negatively charged as CMC or XG-ALG mix layers amounted to (4 ± 1) nm.…”

Section: Resultssupporting

confidence: 69%

“…Mean thickness values determined for ConA films when the polysaccharide surface was negatively charged as CMC or XG-ALG mix layers amounted to (4 ± 1) nm. However, it decreased to ∼(2.5 ± 0.5) nm when the polysaccharide surface was uncharged, as in the case of pure XG [13] or cashew gum [10]. These findings indicate that the adsorption of ConA onto negatively charged polysaccharide surfaces are probably driven by electrostatic binding of lectin positively charged residues to the surface and specific sugar moieties recognition plays a marginal role.…”

Section: Resultsmentioning

confidence: 80%

“…These derivatives are potential supports for biomedical applications due to their biocompatibility and their role in many recognition processes. Complex and variable structures, molecular recognition and specific binding between polysaccharides and proteins open a large field for biosensors and bioassays design [4][5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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Lectins and/or xyloglucans/alginate layers as supports for immobilization of dengue virus particles

Pereira¹,

Sierakowski²,

Jó³

et al. 2008

Colloids and Surfaces B: Biointerfaces

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Lectins and/or xyloglucans/alginate layers as supports for immobilization of dengue virus particles

Pereira¹,

Sierakowski²,

Jó³

et al. 2008

Colloids and Surfaces B: Biointerfaces

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“…2007; Kosaka, Kawano, Salvadori, & Petri, 2005;Maciel, Kosaka, de Paula, Feitosa, & Petri, 2007;Sierakowski, Freitas, Fujimoto, & Petri, 2002;Velzenberger, Kirat, Legeay, Nagel, & Pezron, 2009) or DNA (Sasou, Sugiyama, Ishida, Ohtani, & Miyake, 2009). In this work the effect of surface energy parameters on the immobilization of LYS onto SSP films adsorbed from EmimAc, water and NaH 0.01 mol/L was also investigated.…”

Section: Tablementioning

confidence: 99%

Effect of solvent on the adsorption behavior and on the surface properties of Sterculia striata polysaccharide

Dário

Paula

et al. 2010

Carbohydrate Polymers

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a b s t r a c tCharacterization of Sterculia striata polysaccharide (SSP) films adsorbed onto Si wafers from solutions prepared in ethyl methyl imidazolium acetate (EmimAc), water or NaOH 0.01 mol/L was systematically studied by means of ellipsometry, atomic force microscopy and contact angle measurements. SSP adsorbed from EmimAc onto Si wafer as homogeneous monolayers (∼0.5 nm thick), while from water or NaOH 0.01 mol/L SSP formed layers of ∼4.0 nm and ∼1.5 nm thick, respectively. Surface energy values found for SSP adsorbed from EmimAc or water were 68 ± 2 mJ/m 2 and 65 ± 2 mJ/m 2 , respectively, whereas from NaOH it amounted to 57 ± 3 mJ/m 2 . The immobilization of lysozyme (LYS) onto SSP films was also investigated. The mean thickness of LYS (d LYS ) immobilized onto SSP films adsorbed from each solvent tended to increase with the decrease of p S and total S . However, the enzymatic activity of LYS molecules was higher when they were immobilized onto SSP films with higher p S and total S values.

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“…Fabrication of multilayer films utilizing carbohydrate polymers has been reported for xyloglucan [1], cashew gum [2], chitosan [3], carrageenan [4], alginate [5], amylose [6], and oxidized galactomannan [7].…”

Section: Introductionmentioning

confidence: 99%

Galactomannan thin films as supports for the immobilization of Concanavalin A and/or dengue viruses

Valenga

Petri

Lucyszyn

et al. 2012

International Journal of Biological Macromolecules

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The immobilization of the glucose/mannose-binding lectin from Concanavalia ensiformis seeds (ConA) onto a monolayer made of a galactomannan extracted from Leucaena leucocephala seeds (GML), which was adsorbed onto - amino-terminated surfaces, was investigated by means of ellipsometry and atomic force microscopy. The mean thickness of GML monolayer, which polysaccharide consists of linear 1→4-linked β-D-mannopyranosil units partially substituted at C-6 by α-D-galactopyranosyl units, amounted to (1.5±0.2) nm. ConA molecules adsorbed onto GML surfaces forming (2.0±0.5) nm thick layers. However, in the presence of mannose the adsorption failed, indicating that ConA binding sites were blocked by mannose and were no longer available for mannose units present in the GML backbone. The GML film was also used as support for the adsorption of three serotypes of dengue virus particles (DENV-1, DENV-2 and DENV-3), where DENV-2 formed the thickest film (4±2) nm. The adsorbed layer of DENV-2 onto ConA-covered GML surfaces presented mean thickness values similar to that determined for DENV-2 onto bare GML surfaces. The addition of free mannose units prevented DENV-2 adsorption onto ConA-covered GML films by ~50%, suggesting competition between virus and mannose for ConA binding sites. This finding suggests that if ConA is also adsorbed to GML surface and its binding site is blocked by free mannose, virus particles are able to recognized GML mannose unities substituted by galactose. Interactions between polysaccharides thin films, proteins, and viruses are of great relevance since they can provide basis for the development of biotechnological devices. These results indicate that GML is a potential polysaccharide for biomaterials development, as those could involve interactions between ConA in immune system and viruses.

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Formation of cashew gum thin films onto silicon wafers or amino-terminated surfaces and the immobilization of Concanavalin A on them

Cited by 18 publications

References 35 publications

Lectins and/or xyloglucans/alginate layers as supports for immobilization of dengue virus particles

Lectins and/or xyloglucans/alginate layers as supports for immobilization of dengue virus particles

Effect of solvent on the adsorption behavior and on the surface properties of Sterculia striata polysaccharide

Galactomannan thin films as supports for the immobilization of Concanavalin A and/or dengue viruses

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