Formation of Cytoskeletal Elements During Mouse Embryogenesis

JACKSON, BRIAN W.; GRUND, CHRISTINE; WINTER, STEFANIE; FRANKE, WERNER W.; ILLMENSEE, KARL

doi:10.1111/j.1432-0436.1981.tb01177.x

Cited by 202 publications

(44 citation statements)

References 69 publications

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“…Up-regulation of vimentin (spot 21), a component of intermediate filaments, was also detected at E9.5 and E10.5. This correlates with previous immunofluorescence studies in which vimentin was first detected at late E8 in the mesoderm, and at E9 in the neural tube with significantly increased levels at E10 (19,20). Therefore, components of all three principal cytoskeletal systems, comprising the actin microfilament network, tubulin-containing microtubules and the intermediate filament network (21), were up-regulated at E8.5-10.5.…”

Section: Resultssupporting

confidence: 90%

Differential Protein Expression at the Stage of Neural Tube Closure in the Mouse Embryo

Greene¹,

Leung²,

Wait³

et al. 2002

Journal of Biological Chemistry

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Analysis of the protein complement of a biological system through proteomics provides the opportunity to directly monitor the functional readout of gene expression. In this study, proteomics was applied to the mouse embryo to investigate the molecular events underlying the processes occurring at the stage of neural tube closure. Protein profiles of embryos between embryonic days 8.5 and 10.5 exhibited a number of stage-specific changes. Identification of developmentally regulated proteins by mass spectrometry revealed several groups of functionally related proteins including circulatory, cytoskeletal, and stress proteins. Additional proteins of unknown function were identified, such as Copine 1 and PICOT, whose developmental regulation was previously unsuspected.

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Section: Resultssupporting

confidence: 90%

Differential Protein Expression at the Stage of Neural Tube Closure in the Mouse Embryo

Greene¹,

Leung²,

Wait³

et al. 2002

Journal of Biological Chemistry

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“…Keratin 8 (mK8) and its partner keratin 18 (mK18) are the first IF proteins to be expressed during mouse embryogenesis (Jackson et al 1980;Kemler et al 1981;Oshima et al 1983). They are expressed in several extraembryonic tissues, including the trophectoderm, the endoderm, the visceral yolk sac, and the placenta.…”

mentioning

confidence: 99%

Mid-gestational lethality in mice lacking keratin 8.

Baribault¹,

Price²,

Miyai³

et al. 1993

Genes & Development

243

174

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Keratin 8 (mK8) and its partner keratin 18 (mK18) are the first intermediate filament proteins expressed during mouse embryogenesis. They are found in most extraembryonic and embryonic simple epithelia, including trophectoderm, visceral yolk sac, gastrointestinal tract, lungs, mammary glands, and uterus. We report that a targeted null mutation in the inK8 gene causes mid-gestational lethality. Mutant embryos are growth retarded and suffer from internal bleeding, with an abnormal accumulation of erythrocytes in fetal livers. The mK8-phenotype has 94% penetrance, with a few mice surviving into adulthood. We suggest that mK8/mK18 filaments are important for the integrity of the fetal liver, like specialized human epidermal keratins for the integrity of the epidermis. This phenotype in mice differs from the reported function of simple epithelium keratins in Xenopus at the gastrulation stage. In mice, mK8 fulfills a vital function at 12 days postcoitum.

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“…Later, a second epithelial cell type appears on the surface of the inner cell mass, forming the primitive endoderm (4). These two epithelia express cytokeratin intermediate filaments (5)(6)(7)(8). One of the major components of these intermediate filaments is the protein named cytokeratin endo A, also referred to as cytokeratin A (5,7,9,10).…”

mentioning

confidence: 99%

Expression of the cytokeratin endo A gene during early mouse embryogenesis.

Duprey¹,

Morello²,

Vasseur³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Expression of cytokeratin endo A has been analyzed during mouse blastocyst formation and embryonal carcinoma cell differentiation. To study the regulation of endo A expression, nuclease S1 mapping experiments have been performed on RNA extracted from two-cell to 7.5-day embryos. Low levels of endo A mRNA begin to be detectable in eight-cell embryos. The amount of this mRNA increases at the blastocyst stage, suggesting that endo A expression is regulated at the mRNA level during blastocyst formation. At this stage, in situ hybridization studies show that endo A mRNA is present in the trophectoderm but not in the inner cell mass. In 7.5-day embryos, endo A mRNAs are also detectable in the endoderm layer and in the amnion.

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Formation of Cytoskeletal Elements During Mouse Embryogenesis

Cited by 202 publications

References 69 publications

Differential Protein Expression at the Stage of Neural Tube Closure in the Mouse Embryo

Differential Protein Expression at the Stage of Neural Tube Closure in the Mouse Embryo

Mid-gestational lethality in mice lacking keratin 8.

Expression of the cytokeratin endo A gene during early mouse embryogenesis.

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