Formation of enzymes by biodegradation of agricultural wastes with white rot fungi

El-Nasser, Nadia H Abd; Helmy, Samia M.; El-Gammal, A. A.

doi:10.1016/s0141-3910(96)00117-6

Cited by 29 publications

(6 citation statements)

References 19 publications

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“…Cell-free culture extracts from 2-day solid-substrate fermentation bottles (control and culture) were collected. Determination of specific enzyme activities of five enzymes (α-amylase, glucoamylase, xylanase, endocellulase, and exocellulase) over corn starch, xylan, carboxymethyl cellulose (CMC), and Sigmacell 20 (cellulose) was performed by slightly modifying the procedures of Abd El-Nasser et al and Lee et al . Mixture of 0.25 mL of cell-free extract, 0.25 mL of acetate buffer (0.06M, pH 5), and 0.25 mL of individual substrate (concentration = 3 g/L) were prepared in microcentrifuge vials, and then the vials were placed in a water bath at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Solid-Substrate Fermentation of Corn Fiber by Phanerochaete chrysosporium and Subsequent Fermentation of Hydrolysate into Ethanol

Shrestha

Rasmussen

Khanal

et al. 2008

J. Agric. Food Chem.

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The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungusPhanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37 °C for up to 3 days. Extracellular enzymes produced in situ by P. chrysosporium degraded lignin and enhanced saccharification of polysaccharides in corn fiber. The percentage biomass weight loss and Klason lignin reduction were 34 and 41%, respectively. Anaerobic incubation at 37 °C following 2 day incubation reduced the fungal sugar consumption and enhanced the in situ cellulolytic enzyme activities. Two days of aerobic solid-substrate fermentation of corn fiber with P. chrysosporium, followed by anaerobic static submerged-culture fermentation resulted in 1.7 g of ethanol/100 g of corn fiber in 6 days, whereas yeast (Saccharomyces cerevisiae) cocultured with P. chrysosporium demonstrated enhanced ethanol production of 3 g of ethanol/100 g of corn fiber. Specific enzyme activity assays suggested starch and hemi/cellulose contribution of fermentable sugar. The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungus Phanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37°C for up to 3 days. Extracellular enzymes produced in situ by P. chrysosporium degraded lignin and enhanced saccharification of polysaccharides in corn fiber. The percentage biomass weight loss and Klason lignin reduction were 34 and 41%, respectively. Anaerobic incubation at 37°C following 2 day incubation reduced the fungal sugar consumption and enhanced the in situ cellulolytic enzyme activities. Two days of aerobic solid-substrate fermentation of corn fiber with P. chrysosporium, followed by anaerobic static submerged-culture fermentation resulted in 1.7 g of ethanol/100 g of corn fiber in 6 days, whereas yeast (Saccharomyces cerevisiae) cocultured with P. chrysosporium demonstrated enhanced ethanol production of 3 g of ethanol/100 g of corn fiber. Specific enzyme activity assays suggested starch and hemi/cellulose contribution of fermentable sugar. Keywords

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Section: Methodsmentioning

confidence: 99%

Solid-Substrate Fermentation of Corn Fiber by Phanerochaete chrysosporium and Subsequent Fermentation of Hydrolysate into Ethanol

Shrestha

Rasmussen

Khanal

et al. 2008

J. Agric. Food Chem.

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“…Aeration rate used (3%) may also contributed to the results found. Possibly, if the incubation period was longer, would have been observed a higher hydrolytic enzymes activity Adverse results were noted by Abd El-Nasser et al(1997) studying Phanerochaetae chrysosporium incubation in a liquid medium, performed in stationary system and under agitation. The incubation under agitation results a slight increase of xylanase, glucanase and cellulase activities, when substrate such as sugar cane bagasse, corn cob and wheat straw were used.…”

Section: Determination Of L Edodes Hydrolytic Enzymes Activity In Bimentioning

confidence: 96%

Influence of stationary and bioreactor cultivation on Lentinula edodes (berk) pegler lignocellulolitic activity

Regina

Broetto

Giovannozzi-Sermanni

et al. 2008

Braz. arch. biol. technol.

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“…White-rot basidiomycetes normally secrete large amounts of these enzymes to degrade lignocellulosic materials. For example, Phanerochaete chrysosporium produces high levels of αglucuronidase [101], and Coriolus versicolor produces a complex xylanolytic combination of enzymes [102]. Xylanase is also produced by Cuninghamella subvermispora when growing on plant cell-wall polysaccharides or on wood chips [103].…”

Section: Fungimentioning

confidence: 99%

A Review of Xylanase Production by the Fermentation of Xylan: Classification, Characterization and Applications

Motta¹,

Andrade²,

Santana³

2013

Sustainable Degradation of Lignocellulosic Biomass - Techniques, Applications and Commercialization

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Formation of enzymes by biodegradation of agricultural wastes with white rot fungi

Cited by 29 publications

References 19 publications

Solid-Substrate Fermentation of Corn Fiber by Phanerochaete chrysosporium and Subsequent Fermentation of Hydrolysate into Ethanol

Solid-Substrate Fermentation of Corn Fiber by Phanerochaete chrysosporium and Subsequent Fermentation of Hydrolysate into Ethanol

Influence of stationary and bioreactor cultivation on Lentinula edodes (berk) pegler lignocellulolitic activity

A Review of Xylanase Production by the Fermentation of Xylan: Classification, Characterization and Applications

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