Formation of ethyl acetate from whey by Kluyveromyces marxianus on a pilot scale

Löser, Christian; Urit, Thanet; Stukert, Anton; Bley, Thomas

doi:10.1016/j.jbiotec.2012.10.009

Cited by 56 publications

(70 citation statements)

References 31 publications

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“…Various yeast species can synthesize ethyl acetate (reviewed by Löser et al [9]), but only Pichia anomala, Candida utilis, and Kluyveromyces marxianus produce this ester in larger amounts. K. marxianus is the most promising candidate for large-scale ester production since this dairy yeast with GRAS status grows quickly, converts sugar directly into ethyl acetate without ethanol as an essential intermediate, and produces the ester with a high rate and yield [9][10][11][12][13][14][15][16][17]. The ester synthesis in K. marxianus is easy to control by the level of iron [11,13,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…K. marxianus is the most promising candidate for large-scale ester production since this dairy yeast with GRAS status grows quickly, converts sugar directly into ethyl acetate without ethanol as an essential intermediate, and produces the ester with a high rate and yield [9][10][11][12][13][14][15][16][17]. The ester synthesis in K. marxianus is easy to control by the level of iron [11,13,[16][17][18]. K. marxianus exhibits a distinct thermal tolerance which allows cultivation at an elevated temperature [14,19,20] which in turn accelerates the ester stripping and advances its process-integrated recovery.…”

Section: Introductionmentioning

confidence: 99%

“…A high biomass concentration results in a quick process, but the production of this biomass, on the other hand, is connected with sugar consumption which reduces the portion of sugar available for ester production (as demonstrated in pilot-scale experiments [13]). The right balance between yeast growth and ester synthesis or, in other words, a compromise between a quick process and a high ester yield is of practical importance.…”

Section: Introductionmentioning

confidence: 99%

“…The right balance between yeast growth and ester synthesis or, in other words, a compromise between a quick process and a high ester yield is of practical importance. This balance can be controlled by the available iron [13,16,23].…”

Section: Introductionmentioning

confidence: 99%

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Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate

et al. 2015

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Background: Whey is just turning from a waste of milk processing to a renewable raw material in biotechnology for producing single-cell protein, bio-ethanol, or ethyl acetate as an economic alternative. Conversion of whey-borne sugar into ethyl acetate requires yeast biomass as a biocatalyst. A high cell concentration results in a quick ester synthesis, but biomass growth means consumption of sugar at the expense of ester production. Efficient and cost-saving biomass production is thus a practical requirement. Whey is poor in nitrogen and has therefore to be supplemented with a bioavailable N source. Methods: Several aerobic growth tests were performed with Kluyveromyces marxianus DSM 5422 as a potent producer of ethyl acetate in whey-borne media supplemented with various N sources. Preliminary tests were done in shake flasks while detailed studies were performed in a stirred bioreactor.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate

et al. 2015

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“…However, the recent use of the thermotolerant yeast, Kluy-veromyces marxianus, for the production of biofuels and commercially valuable chemicals from renewable resources provides an alternative to S. cerevisiae [18][19][20]. The thermotolerant K. marxianus has several advantages over the mesophilic yeast S. cerevisiae in a number of practical aspects, for example; reduced cooling costs, improved enzymatic hydrolysis for SSF, a high growth rate, the ability to utilize various substrates and low contamination levels [21,22].…”

Section: Introductionmentioning

confidence: 99%

The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production

Park¹,

Kim²,

Jang³

et al. 2015

KSBB Journal

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Kluyveromyces marxianus is a well-known thermotolerant yeast. Although Saccharomyces cerevisiae is the most commonly used yeast species for ethanol production, the thermotolerant K. marxianus is more suitable for simultaneous saccharification and fermentation (SSF) processes. This is because enzymatic saccharification usually requires a higher temperature than that needed for the optimum growth of S. cerevisiae. In this study, we compared the fermentation patterns of S. cerevisiae and K. marxianus under various temperatures of fermentation. The results show that at a fermentation temperature of 45°C, K. marxianus exhibited more than two fold higher growth rate and ethanol production rate in comparison to S. cerevisiae. For SSF using starch or corn stover as the sole carbon source by K. marxianus, the high temperature (45°C) fermentations showed higher enzymatic activities and ethanol production compared to SSF at 30°C. These results demonstrate the potential of the thermotolerant yeast K. marxianus for SSF in the industrial production of biofuels.

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Advanced downstream processing for sustainable ethyl acetate production by fermentation

Janković,

Straathof,

Kiss

2024

AIChE Journal

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Ethyl acetate is a platform chemical conventionally obtained through fossil fuel routes, but more recently its production by fermentation from carbohydrates has been scaled up to a pilot scale. Yet, the complexity of downstream processing (low product concentrations in liquid broth and in off‐gas, azeotrope formation, and the presence of microorganisms) may complicate industrial application. This original theoretical study is the first to develop advanced downstream processing, based on process intensification principles, for large‐scale recovery (~10 kton/year) of ethyl acetate after fermentation. To minimize product losses, ethyl acetate is separated from both the liquid broth and off‐gas. The final purification is performed in a highly integrated azeotropic dividing‐wall column. The economic and sustainability analysis shows that using refrigeration for initial product separation from the gas phase is more cost‐effective (~0.61 $/kg) and less energy‐intensive (2.20–2.40 kWthh/kg) than compression combined with high‐pressure condensation using chilled water (1.09 $/kg and 9.98 kWthh/kg).

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Formation of ethyl acetate from whey by Kluyveromyces marxianus on a pilot scale

Cited by 56 publications

References 31 publications

Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate

Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate

The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production

Advanced downstream processing for sustainable ethyl acetate production by fermentation

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