Formation of ethyl esters of long chain carboxylic acids

Burfeind, Jens; Schügerl, K.

doi:10.1016/s0032-9592(98)00142-3

Cited by 6 publications

(11 citation statements)

References 10 publications

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“…Aus Sicht der groûmaûstäblichen Anwendung wird die Aufarbeitung groûvolumiger Ganzzell-Biotransformationen durch die Hydrolasebehandlung erheblich vereinfacht und effektiver. Optimierte Verfahren gestatten den Einsatz kontinuierlicher Extraktionsverfahren, wie zum Beispiel mit Mixer-Settler-Einheiten [11,12].…”

Section: Bedeutung Der Ergebnisse Für Die Praxisunclassified

Neuartiges Verfahren zur extraktiven Isolierung von organischen Komponenten aus Ganzzell‐Biotransformationen

Jörg

Leppchen

Bertau³

et al. 2004

Chemie Ingenieur Technik

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Section: Bedeutung Der Ergebnisse Für Die Praxisunclassified

Neuartiges Verfahren zur extraktiven Isolierung von organischen Komponenten aus Ganzzell‐Biotransformationen

Jörg

Leppchen

Bertau³

et al. 2004

Chemie Ingenieur Technik

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“…In case of hydrophobic microporous membranes, the water phase has an overpressure (0.3 bar) in order to avoid the break through of the solvent phase [32]. Concerning hydrophilic micro®ltration membranes, the pores are ®lled with the aqueous phase and the solvent phase must have an overpressure.…”

Section: Membrane Extractor Systemmentioning

confidence: 99%

“…The extracted solute (the product of the bioprocess) is removed from the organic phase by evaporation [32] or enzymatic reaction (see the next chapter).…”

Section: Membrane Extractor Systemmentioning

confidence: 99%

“…In most frequent con®gurations of the membrane extractors are the¯at sheet membranes [29,33] and the hollow ®bres [26,27], but there are examples for membrane tubes wound spirally in the device [32].…”

Section: Membrane Extractor Systemmentioning

confidence: 99%

“…In multiphase enzyme membrane reactors the organic phase is often the substrate itself (e.g. oleic acid [32,50]). …”

Section: Solvent Selection Extraction Conditions Water Activity Conmentioning

confidence: 99%

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Technical aspects of separation and simultaneous enzymatic reaction in multiphase enzyme membrane reactors

Sisak

Nagy

Burfeind

et al. 2000

Bioprocess Engineering

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The technical aspects of the membrane extraction of a compound either from aqueous phase into apolar organic solvent phase or from the apolar phase to the aqueous one and the enzymatic conversion of the solute in a multiphase enzyme membrane reactor are considered. The application possibilities, the selection aspects of membrane material as well as the solvent phase, the water content and its control, the method of the enzyme immobilisation and the operation of the extraction/reaction system are discussed.

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A novel convenient procedure for extractive work‐up of whole‐cell biotransformations using de‐emulsifying hydrolases

Jörg

Leppchen

Daußmann

et al. 2004

Biotech & Bioengineering

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Extractive work-up of whole-cell biotransformations generally suffers from the formation of stable gels and slimes upon addition of the organic solvent to the cell suspension and the cell-free solution, respectively. This problem has been overcome by enzymatic lysis of emulsifying agents present in the medium through addition of hydrolases. Of these agents, proteases have exhibited the most powerful de-emulsifying activity. Enzyme treatment of cell-free culture media of Saccharomyces cerevisiae with pronase E drastically reduced phase separation time (t(p)) from 1 week to 30 min without significantly affecting product integrity. Yeast cell suspensions were de-emulsified best with protease N-01, where phase separation was complete after 1 h. As was exemplified with cell-free culture media of Lactobacillus kefir, wherein addition of pronase E or protease N-01 reduced t(p) from 1 week to 2 h each, this practical, ready-to-use method is appropriate for both fungal and bacterial biocatalysts.

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Formation of ethyl esters of long chain carboxylic acids

Cited by 6 publications

References 10 publications

Neuartiges Verfahren zur extraktiven Isolierung von organischen Komponenten aus Ganzzell‐Biotransformationen

Neuartiges Verfahren zur extraktiven Isolierung von organischen Komponenten aus Ganzzell‐Biotransformationen

Technical aspects of separation and simultaneous enzymatic reaction in multiphase enzyme membrane reactors

A novel convenient procedure for extractive work‐up of whole‐cell biotransformations using de‐emulsifying hydrolases

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