Formation of glycyrrhizin by in vitro cultures of Glycyrrhiza glabra

Shams-Ardakani, Mohammadreza; Mohagheghzadeh, Abdolali; Ghannadi, Alireza; Barati, Azar

doi:10.1007/s10600-007-0132-z

Cited by 13 publications

(4 citation statements)

References 11 publications

(3 reference statements)

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“…Production of glycyrrhizin in cell culture of licorice has been attempted by several workers. Glycyrrhizin was not detected in cell culture studies undertaken by Hayashi et al (1988), Arias-Castro et al (1993), and Toivonen and Rosenqvist (1995), except studies conducted by Tamaki et al (1973) and Shams-Ardakani et al (2007) who reported 1.85% and 2.71% glycyrrhizin from Glycyrrhiza glabra cell culture, respectively. Thus, its production from alternative sources has assumed significance.…”

Section: Introductionmentioning

confidence: 87%

Elicitation as yield enhancement strategy for glycyrrhizin production by cell cultures of Abrus precatorius Linn.

Karwasara

Jain

Tomar

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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Glycyrrhizin is an important phytoconstituent of licorice which is widely used in the pharmaceutical and food industry. As the roots and leaves of Abrus precatorius also contain glycyrrhizin, it can be used as an alternative source of glycyrrhizin. In spite of extensive research work undertaken with cultures of Glycyrrhiza glabra, the glycyrrhizin production remains elusive. Successful production of glycyrrhizin in cell cultures of A. precatorius is being reported for the first time in our study. Cell cultures of A. precatorius L. were treated with the elicitors prepared from the fungi (Aspergillus niger and Rhizopus stolonifer), yeast extract, salicylic acid, ascorbic acid, and eugenol to induce and enhance the synthesis of glycyrrhizin. In the present study, an integrated yield enhancement strategy, developed by the addition of selected elicitor (A. niger and ascorbic acid) at optimized concentrations, resulted in 24.6 g/l dry cell weight biomass and 53.62 mg/l glycyrrhizin, which was 5.22 times higher in productivity in comparison to control cultures.

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Section: Introductionmentioning

confidence: 87%

Elicitation as yield enhancement strategy for glycyrrhizin production by cell cultures of Abrus precatorius Linn.

Karwasara

Jain

Tomar

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…This was maybe because nearly all of the ascorbic acid was converted into dehydroascorbic acid at later time. [21] In general, ascorbic acid turned out to be effective to control the browning. L-cysteine was another effective inhibitor of the browning.…”

Section: Inhibitory Effects Of Anti-browning Agentsmentioning

confidence: 99%

Study on enzymatic browning in suspension cultures of licorice cells

Meng

Wang

et al. 2015

Biotechnology & Biotechnological Equipment

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Enzymatic browning is one of the main obstacles encountered in the establishment of suspension systems of licorice cells. Browning of cells may result in decreased viability, poor growth and even death. The present study investigated the mechanism of browning reactions and the effective controlling methods. The results showed that the cell viability and membrane permeabilization obviously changed when the cells were transferred to liquid medium. The transformation caused rapid increase in the levels of polyphenol oxidase activity and in the production of polyphenols. Osmotic and hydrodynamic stresses arising from liquid culture were regarded as the major causes of enzymatic browning. Ascorbic acid and L-cysteine were found to be the most significant antibrowning agents that could decrease the degree of browning with 55.8% and 52.2%, respectively, at the end of the suspension culture's lag phase. When cultured with a cycle of 21 days, the maximum biomass of the cells cultured with ascorbic acid and L-cysteine increased with 31.1% and 26.5%, respectively, when compared to the control. These findings may be essential for the development of licorice cell cultures devoted to browning prevention and cell viability maintaining. KEYWORDS Licorice (Glycyrrhiza inflata Batalin); polyphenol oxidase; anti-browning agents Abbreviations PPO polyphenol oxidase 6-BA 6-benzyladenine 2,4-D 2,4-dichlorophenoxyacetic acid NAA naphthaleneacetic acid TTC 2,3,5-triphenyltetrazolium chloride MS Murashige and Skoog SDS sodium dodecyl sulfate wt weight OD optical density EDTA-Na 2 disodium ethylenediaminetetraacetate dihydrate LSD Fisher's least significant difference GAE gallic acid equivalent VC ascorbic acid CONTACT Yali Li

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“…2A. other workers have reported similar successful induction of G. glabra and G. uralensis (17,18). The calli were subcultured every month on the same medium to obtain the biomass accumulation of G. inflata cells.…”

Section: Resultsmentioning

confidence: 92%

Production of Glycyrrhizin in Cell Suspension ofGlycyrrhiza InflataBatalin Cultured in Bioreactor

Yang

et al. 2012

Biotechnology & Biotechnological Equipment

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Formation of glycyrrhizin by in vitro cultures of Glycyrrhiza glabra

Cited by 13 publications

References 11 publications

Elicitation as yield enhancement strategy for glycyrrhizin production by cell cultures of Abrus precatorius Linn.

Elicitation as yield enhancement strategy for glycyrrhizin production by cell cultures of Abrus precatorius Linn.

Study on enzymatic browning in suspension cultures of licorice cells

Production of Glycyrrhizin in Cell Suspension ofGlycyrrhiza InflataBatalin Cultured in Bioreactor

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