Formation of Homoserine in Germinating Pea Seeds.

Berg, A.; Kari, Sigrid; Virtanen, Artturi I.; Sørensen, Nils Andreas

doi:10.3891/acta.chem.scand.07-1423

Cited by 43 publications

(10 citation statements)

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“…15, on the other hand, was present in large amounts in materials released only from wheat. Homoserine, an amino acid found in germinating pea seedlings by Virtanen et al 16 and detected in pea root exudate by Rovira s was found in the present study only in peas grown in sand, but not in solution culture of either wheat or peas. Boulter et al i. detected homoserine in solution culture of pea seedlings but found disproportionately greater quantities released in sand cultures.…”

Section: Qualitative Amino Acid Patterns/rom Roots In Solution Culturessupporting

confidence: 34%

Exudation of amino acids by intact and damaged roots of wheat and peas

Ayers

Thornton

1968

Plant Soil

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Section: Qualitative Amino Acid Patterns/rom Roots In Solution Culturessupporting

confidence: 34%

Exudation of amino acids by intact and damaged roots of wheat and peas

Ayers

Thornton

1968

Plant Soil

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“…A remarkably close parallel is found in the behaviour of homoserine during the early stages of growth of the pea plant (Virtanen, Berg & Kari, 1953). The hydroxy acid is absent from the dormant seed, can be detected 24 hr.…”

Section: (A) Amino Acid Concentrations In Relation To Plant Developmentsupporting

confidence: 57%

New Amino Acids of Plants

Fowden¹

1958

Biological Reviews

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“…In the complete analyses reported here, there was at least as much arginine and asparagine present as glutamic acid in the seeds initially and after one day's germination, after which homoserine rose to predominance. as has been noted by others (15,20). The changes in amount of some of the more quantitatively important amino acids in the total seedling tissue have 320 406 318 33 25 103 75 100 102 45 1710 324 373 268 1360 454 122 307 187 101 17 4440 332 299 488 2846 491 233 339 196 126 37 6450 100 seedlings, with seed been summed up in table III.…”

Section: Discussionmentioning

confidence: 64%

“…Semiquantitative analyses by paper chromatography have been reported for many of the free amino acids in pea seeds (9,16,20), whole seedlings at various growth stages (20) and seedling cotyledons and axis tissue at different growth stages (16).…”

Section: Discussionmentioning

confidence: 99%

Nitrogen Mobilization in Pea Seedlings. II. Free Amino Acids

Lawrence

Grant

1963

Plant Physiol.

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In an earlier report from this laboratory (10) the changes in protein nitrogen and various forms of nonprotein nitrogen-amino, amide, peptide, ammonia, and urea-were reported for the different tissues of peas and pea seedlings during the early stages of growth. The data emphasize the quantitative importance of the free amino acids, particularly in the roots and shoots of these young seedlings. Although they contribute only about 3 % to the total nitrogen of the seed, their contribution rises to 10 % in the 5-day seedling. In root shaft and shoot shaft tissue of the 5-day seedling, from 40 to 45 % of the nitrogen is in the free amino acids. The work did not make clear the role these amino acids plav in the conversion of the storage proteins of the seed to the functional proteins of the young plant. Clearly the first information needed for this purpose is a dcetailed picture of the changes in the concentration of the individual amino acids. For the former method, the extracts were evaporated to dryness under vacuum, and taken up either in water followed by adjustment to about pH 2 with a few drops of HCl, or in citrate buffer of pH 2.35 (11). At this stage, the cotyledon extracts were filtered and made to a convenient volume for the ion exchange chromatography. The other extracts, however, gave better chromatographic separations, if they were first extracted with petroleum ether before filtration and dilution to a convenient volume.The method of Rosen (13) was used with minor modifications to determine the ninhydrin color yield of the fractions from the column. The concentration of the ninhydrin reagent was increased to 5 %, since the 2 ml fractions from the fraction collector resulted in increased dilution of the reaction mixture. Twice the recommended volume of the 50 % isopropanol diluent was used. We found the cyanide-acetate solution not to be stable (7) in that a lower ninhydrin color was obtained if the solution used was more than 4 hours old. Hence the cyanide and acetate components were mixed just before use. The fractions, except for the less acidic ones from the 15 cm column for the basic amino acids, were partially neutralized with 2 drops of 2 N NaOH before treatment with the reagents. Standard leucine samples were run at regular intervals to check the performance of the method.For the automatic method, the extracts were evaporated to dryness under vacuum, and simply dissolved up to a convenient volume in pH 2.91 citrate buffer (12). In general, the results by the 2 methods agreed well, with the exceptions mentioned below, and were averaged together for the results presented in table I. Amino acids were determined on hydrolysates of some of the residues from the 70 % alcohol extractions. The residues containing 8 to 25 mg of nitrogen were hydrolzed by autoclaving them at 121°for 5 hours with 25 ml of 3 N HCl. The amino acid contents of aliquot portions were determined using the manual (fraction collector) method.

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Formation of Homoserine in Germinating Pea Seeds.

Cited by 43 publications

References 0 publications

Exudation of amino acids by intact and damaged roots of wheat and peas

Exudation of amino acids by intact and damaged roots of wheat and peas

New Amino Acids of Plants

Nitrogen Mobilization in Pea Seedlings. II. Free Amino Acids

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