Formation of Ice Nucleation-active Vesicles in<i>Erwinia uredovora</i>at Low Temperature and Transport of InaU Molecules into Shed Vesicles

Michigami, Yasuyuki; Abe, Keiko; Iwabuchi, Kyoko; Obata, Hitoshi; Arai, Soichi

doi:10.1271/bbb.59.1996

Cited by 11 publications

(8 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed vesicles at the cell walls of both isolates (Figure 1). Vesicles were similar to those described by Phelps et al (1986), Michigami et al (1995), and Kawahara (2002) in other bacterial species as being responsible for the formation of ice nuclei. The vesicles appeared in greater numbers when the bacterium was cultured in medium supplemented with glycerol ( Figure 1B and C).…”

Section: Resultssupporting

confidence: 81%

Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

et al. 2014

View full text Add to dashboard Cite

Maize white spot lesions caused by Pantoea ananatis has contributed substantially to yield reduction of maize crops in many countries, including Brazil. The initial symptoms of the disease include watersoaked lesions on the leaves, which later become necrotic and straw-colored. Basic knowledge regarding the biology and the infection mechanisms of this pathogen is lacking. In this study, 15 P. ananatis isolates obtained from maize white spot lesions were examined for their ice nucleation activity (INA). The INAs of individual bacterial isolates was determined by tube nucleation tests. Bacterial isolates were grown on tryptic soy broth medium and an aliquot of 0.1 mL of culture was added to test tubes containing 1 mL of sterile distilled water. The tubes were packed in an ice bath, which had a temperature below -10°C, for approximately 2 min. Instantaneous formation of ice in the tube revealed a positive INA phenotype of the isolate. Only 9 of the 15 studied isolates showed the INA + phenotype. Pathogenicity tests were performed using whole plants and detached leaves. Symptoms were reproduced in both tests, but only for the inoculations using INA + isolates. Electron microscopy allowed visualization of protein vesicles under outer cell wall of isolates characterized as INA + . Key words: INA, vesicle protein, transmission electron microscopy ResumoLesões de mancha branca do milho causadas por Pantoea ananatis contribuiram substancialmente para a redução da produtividade nos cultivos de milho, em muitos países, incluindo o Brasil. Os sintomas iniciais da doença incluem lesões anasarcas nas folhas, que mais tarde tornam-se necróticas e de cor palha. O conhecimento básico sobre a biologia e os mecanismos de infecção deste patógeno são escassos. Neste estudo, 15 isolados de P. ananatis obtidos a partir de lesões da mancha branca do milho foram examinados quanto à sua atividade de nucleação de gelo (INA). A INA de isolados bacterianos individuais foi determinada por testes de nucleação em tubos. Isolados bacterianos foram cultivados em meio de caldo de soja tríptico e uma alíquota de 0,1 mL de cultura foi adicionada à tubos de ensaio contendo 1 mL de água destilada esterelizada. Os tubos foram colados em banho de gelo, o qual continha temperatura inferior a -10 ºC, por aproximadamente 2 min. A formação de gelo instantânea no tubo revelou um fenótipo INA positivo do isolado. Apenas 9 dos 15 isolados estudados apresentaram o fenótipo INA+. Testes de patogenicidade foram realizados com plantas inteiras e folhas destacadas.

show abstract

Section: Resultssupporting

confidence: 81%

Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

et al. 2014

View full text Add to dashboard Cite

show abstract

“…5). Michigami et al (1995) have reported that the INP first assembles around the inner membrane, then the assembly enters a vesicle just forming on the surface of the outer membrane. During the process of the EIM secretion, we could postulate the following hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, some researchers have reported that for the secretion systems of proteins without signal peptides, various gram-negative bacteria have an ABC transporter system that is required for ATP (Fath and Colter, 1993). Michigami et al (1995) reported that after INP first assembles around the inner membrane, the assembly enters a vesicle just forming on the surface of the outer membrane and leaves the surface to be shed with Ina U molecules as shown by immunohistochemical analysis using electron microscopy. We have reported that polyamines are required for the ice-nucleating activities of the cell and EIM from strain KUIN-3 (Kawahara et al, 1993.…”

mentioning

confidence: 99%

The Control of the Production and Secretion of Extracellular Ice-nucleating Material of Erwinia uredovora KUIN-3.

Kawahara

Matsushita²,

Yamade³

et al. 1999

Biocontrol Sci.

Self Cite

View full text Add to dashboard Cite

“…also detected extracellular ice nuclei when E. uredovora was cultured at 18°C. ECINs exhibited strong INA in cell-free filtrates from cultures grown at 10°C, and no activity was recovered in filtrates from cells grown at 26°C (Michigami et al, 1995a).…”

Section: Discussionmentioning

confidence: 95%

“…Ice nucleation proteins from various bacterial species have related internally repetitive primary structures (Michigami et al, 1995a;Schmid et al, 1997;Warren and Wolber, 1991). Each protein has three domains: a central repeating domain of 80% total sequence with an 8-amino acid residue period of repetition, a unique N-terminal domain, and a unique C-terminal domain.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of extracellular ice nucleators from Erwinia herbicola.

Lee

1998

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Formation of Ice Nucleation-active Vesicles inErwinia uredovoraat Low Temperature and Transport of InaU Molecules into Shed Vesicles

Cited by 11 publications

References 4 publications

Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

The Control of the Production and Secretion of Extracellular Ice-nucleating Material of Erwinia uredovora KUIN-3.

Enhanced production of extracellular ice nucleators from Erwinia herbicola.

Contact Info

Product

Resources

About