Formation of in vivo tissue engineered human hyaline cartilage in the shape of a trachea with internal support

Ruszymah, B H I; Chua, Kien Hui; Latif, Mazlyzam Abdul; Hussein, Fuzina Nor; Saim, Aminuddin Bin

doi:10.1016/j.ijporl.2005.04.026

Cited by 58 publications

(33 citation statements)

References 20 publications

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“…As opposed to degradable biomaterials, non-degradable ones have the advantage of providing both durable mechanical properties and long-term chemical stability. Non-degradable biomaterials have been used for a wide range of biomedical applications (Chirila, 2001;Dziubla et al, 2001;Ruszymah et al, 2005;Xue and Greisler, 2003), but have only recently been investigated for ear cartilage engineering (Lee et al, 2011;Ruszymah et al, 2011;Zhou et al, 2011). Lee et al (Lee et al, 2011) and 6 Ruszymah et al (Ruszymah et al, 2011) investigated, in vivo, the use of commercial HDPE 1 implants covered with cell-fibrin constructs, and Zhou et al (Zhou et al, 2011) studied in vivo the potential of type I collagen implants reinforced with coiled titanium wires.…”

Section: Introductionmentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

202

109

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3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Secondly, the capacity of BNC to match these requirements is assessed. Finally, a biofabrication process to produce patient-specific BNC auricular implants is demonstrated.BNC samples (n = 78) with varying cellulose content (2.5 -15%) were compared using stressrelaxation indentation with human ear cartilage (n = 17, from 4 males, aged 49 -93 years old).Additionally, an auricle from a volunteer was scanned using a 3T MRI with a spoiled gradientecho sequence. A negative ear mold was produced from the MRI data in order to investigate if an ear-shaped BNC prototype could be produced from this mold.The results show that the instantaneous modulus E in , equilibrium modulus E eq , and maximum stress σ max of the BNC samples are correlated to effective cellulose content. Despite significantly different relaxation kinetics, the E in , E eq and σ max of BNC at 14% effective cellulose content reached values equivalent to ear cartilage (for E eq , BNC: 2.4 ± 0.4 MPa and ear cartilage: 3.3 ± 1.3 MPa). Additionally, this work shows that BNC can be fabricated into patient-specific auricular shapes. In conclusion, BNC has the capability to reach mechanical properties of relevance for ear cartilage replacement, and can be produced in patient-specific ear shapes.

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Section: Introductionmentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

202

109

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“…16 Numerous surgical proceedings of tracheal replacement, including prostheses, autologous and heterologus tissues have been used, but none of them has properly replaced the injured trachea. 9,18 Moreover, there is no appropriate alloplastic material developed until the present moment and its possible use in reconstructive surgery of large segment trachea defects would bring about positive long-term experimental results.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6][7] However, in cases of extensive longitudinal stenosis a tracheotomy below to the stricture area could be necessary and the stenosis treated with orthesis, repeated mechanical dilations, 4 implant of biological 8 or synthetic tissues 9 , prosthesis, [10][11][12][13] or recently with the use of homologues or heterologues tracheal transplantation. [14][15][16] The surgical treatment for extensive stenosis of trachea is not a consensus amongst numerous and controversial surgical proceedings. The purpose of this research is to create an animal model of extensive longitudinal TS that should be useful to test different surgical techniques of tracheal reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Model of extensive and severe tracheal stenosis in dogs

et al. 2008

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Purpose:To create an animal model of extensive longitudinal tracheal stenosis (TS) that can be useful to test different surgical techniques of tracheal reconstruction. Methods: Twenty male mongrel dogs were submitted to standard TS and randomly distributed to observation for 3 weeks (n=10) or 6 weeks (n=10). Under general anesthesia, an elliptical area (major axis from 1 st to 20 th ring and minor axis 40% of tracheal diameter) was resected and the stumps were sutured. The internal and external diameters were measured (before and after the observation time) and the stenosis index was calculated. Blood samples were collected (gasometry, hematocrit and hemoglobin.) before and after the surgical proceedings. Results: The weight was significant lower in the animals of 6 weeks (15,551±3286.2) in comparison with those of 3 weeks observation (17,250±3575.0). No significant differences were noted in the extension of the trachea on the 21 st day (21.2± 1.8) or 42 nd day (21.1±1.7). The mean (40.1) and the median (40.5) of rings counted on the 21 st day were quite similar to mean (38.1) and median (39.0) that were counted on the 42 nd day. In the animals of group A (3 weeks) the mean (46.8%) and the median (49.8%) of index stenosis showed no significant difference (Mann Whitney test p<0.001) in comparison with the mean (55.1%) and median (52.4%) of the animals from group B (6 weeks). No mechanical or biochemical distresses were recorded through all period of observation. Conclusion: The surgical proceeding was effective to promote a model of longitudinal and extensive tracheal stenosis. Key words: Tracheal Stenosis. Models, Animal. Dogs. RESUMOObjetivo: Criar um modelo de estenose longitudinal extensa que possa ser útil para testar diferentes técnicas operatórias de reconstrução traqueal. Métodos: 20 cães sem raça definida foram submetidos a um procedimento padrão de estenose traqueal extensa e observados por três (n=10) ou seis (n=10) semanas. Sob anestesia geral uma área elíptica (eixo maior do 1º ao 20º anel traqueal e eixo menos com 40% do diâmetro da luz traqueal) foi ressecada e os cotos foram anastomosados de modo término-terminal. O diâmetro interno e externo foi medido (antes e depois do período de observação) e o índice de estenose foi determinado. Amostras de sangue foram coletadas (gasometria, hematócrito e hemoglobina) antes e depois dos procedimentos operatórios. Resultados: O peso foi significantemente menor nos animais de seis semanas (15,551±3286.2) em comparação com os de três semanas (17,250±3575.0). Não houve diferenças significantes entre a extensão da traquéia com três (21.2± 1.8) ou seis (21.1±1.7) semanas. A média (40.1) e a mediana (40.5) dos anéis contados na 3ª semana foi similar à média (38.1) e mediana (39.0 da 6ª semana. A média (46.8%) e mediana (49.8%) do índice de estenose com três semanas não mostrou diferenças significantes (Mann Whitney test p<0.001) com a média 55.1%) e mediana de seis semanas (52.4%). Nenhum sofrimento mecânico ou bioquímico da respiração foi assinalado. Conc...

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“…The cartilage was diced finely using a scalpel blade to improve the efficiency of subsequent enzymatic digestion. The diced cartilage tissue was digested for 4 hours at 37˚C in collagenase type II solution (0.6%; Worthington, USA) under continuous agitation (Ruszymah et al 2005). After digestion, the suspension containing isolated chondrocytes was centrifuged (Hettich Centrifuges, Germany) at 5000 rpm for 5 min to obtain a cell pellet.…”

Section: Chondrocytes Isolationmentioning

confidence: 99%

Comparative Study on Cartilage Tissue Collected From Less- and Severely-Affected Region of Osteoarthritic Knee

Roy¹

2018

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Formation of in vivo tissue engineered human hyaline cartilage in the shape of a trachea with internal support

Cited by 58 publications

References 20 publications

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Model of extensive and severe tracheal stenosis in dogs

Comparative Study on Cartilage Tissue Collected From Less- and Severely-Affected Region of Osteoarthritic Knee

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