Formation of Micro and Nanoscale Patterns of Monolayer Templates for Position Selective Immobilization of Oligonucleotide Using Ultraviolet and Electron Beam Lithography

Niwa, Daisuke; Omichi, Kaoru; Motohashi, Norikazu; Homma, Takayuki; Osaka, Tetsuya

doi:10.1246/cl.2004.176

Cited by 19 publications

(12 citation statements)

References 9 publications

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“…The water contact angle of the ODMS-modified surface was approximately 95º, indicating that the surface was successfully modified by hydrophobic ODMS. By using ellipsometry, we found that the thickness of the ODMS layer was approximately 20 Å, which corresponds to the thickness of the ODMS monolayer specified in a previous report 13 . The recombinant Mms6 and BSA were then attached to the substrate through the hydrophobic interactions between these proteins and the ODMS monolayer that have been reported previously 14 .…”

Section: Modification Of the Silicon Substrate With Mms6supporting

confidence: 79%

Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

2009

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Mms6 is a small acidic protein which is tightly bound to magnetite in the bacterium Magnetospirillum magneticum AMB-1. Mms6 has been previously shown to promote ironbinding capacity as well as modulate the size and morphology of magnetic iron oxide crystals in vitro. In this study, we synthesized iron oxide crystals by using a monolayer-modified substrate. A self-assembled monolayer of octadecyltrimethoxysilane was modified on a silicon substrate. Recombinant Mms6 protein was attached to the substrate through the hydrophobic interactions between the protein molecules and the monolayer. The immobilization of protein molecules on the substrate surface was confirmed by fluorescent labeling of these molecules and subsequent fluorescence microscopy. This protein-modified substrate was then used as a template for iron oxide crystal formation in a ferrous solution. Scanning electron microscopy revealed sitespecific formation of iron oxide crystals in substrate regions with immobilized proteins. This use of proteins might provide an alternative method for the bottom-up fabrication of nano-sized magnetic particles.

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Section: Modification Of the Silicon Substrate With Mms6supporting

confidence: 79%

Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

2009

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“…For patterning of the molecules, methods including microcontact printing, ink-jet delivery, dip-pen nanolithography, and nano-manipulation using scanning probe microscopy have been proposed. On the other hand, we investigated the process for fabricating molecular templates at micro and nano scales using UV and electron-beam lithography [5,38,39]. These processes aid the controlled formation of SAMs having various functional groups under self-assembling conditions, with a precise position selectivity to form active and passive sites for immobilization of biomolecules.…”

Section: Attachment Of Biomolecules Onto the Recognition Regionmentioning

confidence: 99%

Micro pH Sensors and Biosensors Based on Electrochemical Field Effect Transistors

Sasano

Niwa

Osaka

2009

Nanostructure Science and Technology

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A study on ion-sensing using field effect transistor (FET) was begun by Bergveld in the 1970s [1][2][3]. The ion-sensitive (IS) FET is now widely used as a miniaturized pH sensor, commercialized by some companies. First, the principle and structure of the ISFET are introduced in this section. A basic design of ISFET is shown in Fig. 10.1a. ISFET has silicon substrate with field-effect structures such as electrolyte/IS layer/(insulator)/semiconductor structures; the space charge region in the semiconductor is modulated depending on the gate voltage (V g ), same as a typical metal-oxide-semiconductor (MOS) FET. A typical bias V g versus drain-source current (I ds ) characteristic of the device that has silicon nitride/silicon dioxide/silicon is shown in Fig. 10.1b. This characteristic is quite similar to the MOSFET. A prominent difference between ISFET and MOSFET is that the gate voltage for the operation of the device is applied by an electrochemical reference electrode through the electrolyte in contact with the gate insulator. The threshold voltage (V th ) could shift according to the value of the pH of the solution. In the MOSFET, the V th would shift

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“…Nanopatterning of SAM combined with the EBL produces an organic template for various nanofabrication of biosensors [83, 94]. Biomolecules such as DNAs and proteins are immobilized on nano-patterned organic templates provided by EBL [95, 96]. DNA could be immobilized via sequence-specific DNA hybridization on EBL-nanopatterned surfaces.…”

Section: Nanoscale Patterning Of Biomolecules Using Molecular Recognimentioning

confidence: 99%

Molecular Recognition and Specific Interactions for Biosensing Applications

Kim

Kang

2008

Sensors

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Molecular recognition and specific interactions are reliable and versatile routes for site-specific and well-oriented immobilization of functional biomolecules on surfaces. The control of surface properties via the molecular recognition and specific interactions at the nanoscale is a key element for the nanofabrication of biosensors with high sensitivity and specificity. This review intends to provide a comprehensive understanding of the molecular recognition- and specific interaction-mediated biosensor fabrication routes that leads to biosensors with well-ordered and controlled structures on both nanopatterned surfaces and nanomaterials. Herein self-assembly of the biomolecules via the molecular recognition and specific interactions on nanoscaled surfaces as well as nanofabrication techniques of the biomolecules for biosensor architecture are discussed. We also describe the detection of molecular recognition- and specific interaction-mediated molecular binding as well as advantages of nanoscale detection.

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Formation of Micro and Nanoscale Patterns of Monolayer Templates for Position Selective Immobilization of Oligonucleotide Using Ultraviolet and Electron Beam Lithography

Cited by 19 publications

References 9 publications

Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

Micro pH Sensors and Biosensors Based on Electrochemical Field Effect Transistors

Molecular Recognition and Specific Interactions for Biosensing Applications

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