2017
DOI: 10.1039/c7cp01201b
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Formation of modulated phases and domain rigidification in fatty acid-containing lipid membranes

Abstract: We investigated the phase behavior of lipid membranes containing fatty acids (FAs) by microscopy and differential scanning calorimetry. We used palmitic acid (saturated FA), oleic acid (cis-isomer of unsaturated FA), elaidic acid (trans-isomer of unsaturated FA), and phytanic acid (branched FA) and examined the effects of FAs on phase-separated structures in lipid bilayer membranes consisting of dioleolylphosphocholine (DOPC)/dipalmitoylphosphocholine (DPPC)/cholesterol (Chol). Palmitic acid and elaidic acid e… Show more

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Cited by 24 publications
(49 citation statements)
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“…The line tension values are summarized in Figure 5 . Without LAs, the line tension was about 3 pN at room temperature (20–22 °C), and this value is in good agreement with that reported in previous studies [ 31 , 32 ]. As the temperature increases, the line tension decreases just before reaching the miscibility temperature.…”
Section: Resultssupporting
confidence: 92%
See 1 more Smart Citation
“…The line tension values are summarized in Figure 5 . Without LAs, the line tension was about 3 pN at room temperature (20–22 °C), and this value is in good agreement with that reported in previous studies [ 31 , 32 ]. As the temperature increases, the line tension decreases just before reaching the miscibility temperature.…”
Section: Resultssupporting
confidence: 92%
“…Line tension at the domain boundary is one of the most important factors affecting the stability of phase-separated domains. Generally, the line tension at the L o domain boundary is about ~3 pN [ 31 , 32 ]. Here, we obtained the line tension at the L o domain boundary in LA-containing lipid membranes by the flicker spectroscopy of domain boundary fluctuations.…”
Section: Resultsmentioning
confidence: 99%
“…Among unsaturated fatty acids, NMI fatty acids , whose peculiar unsaturation pattern makes the molecule less flexible with respect to commonly unsaturated fatty acids (Rabinovich and Ripatti, 1991) and, as far as we are aware, lack in mammalian membranes (Barnathan, 2009), approximately attain the same percentage on total fatty acids in clam and mussel mitochondria . However, on considering the membrane arrangement, it seems worthwhile noticing that, while saturated fatty acids tend to exclude cholesterol, due to their straight shape and tight packing, unsaturated fatty acids are well compatible with the bulky sterol insertion (Shimokawa et al, 2017). So, a physical link exists between unsaturated fatty acid and sterol content, since highly unsaturated acyl chains seem to favor sterol incorporation.…”
Section: Lipid Features and Clues On The Membrane Arrangementmentioning
confidence: 99%
“…10 B). In our experiments, we achieve low line tension between L o and L d phases by adjusting the ratio of the three membrane components; other researchers have achieved this effect by gradually replacing one of the three components with a fourth component (30,(35)(36)(37).…”
Section: Domain Morphology Varies With Guv Lipid Compositionmentioning
confidence: 85%