Formation of motile assembly of microtubules driven by kinesins

Kawamura, Ryuzo; Kakugo, Akira; Shikinaka, Kazuhiro; Osada, Yoshihito; Gong, Jian Ping

doi:10.1088/0964-1726/20/12/124007

Cited by 6 publications

(5 citation statements)

References 55 publications

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“…In order to develop a robustly controlled molecular swarm system, we systematically investigated the effect of related physicochemical parameters on the DNA assisted swarming of a self-propelled biomolecular motor system, MT-kinesin. We found that the effects are comparable to those discovered in previous studies where self-assembly of MTs was regulated using streptavidin (St)-biotin (Bt) crosslinkers system 32 – 34 . Unlike the self-assembly of MTs using the strong St-Bt interaction, where MTs assembled even when they moved in opposite direction 32 , 33 , almost no sticking of MT swarms on the kinesin-coated surface was noticed in the present system.…”

Section: Discussionsupporting

confidence: 87%

“…We found that the effects are comparable to those discovered in previous studies where self-assembly of MTs was regulated using streptavidin (St)-biotin (Bt) crosslinkers system 32 – 34 . Unlike the self-assembly of MTs using the strong St-Bt interaction, where MTs assembled even when they moved in opposite direction 32 , 33 , almost no sticking of MT swarms on the kinesin-coated surface was noticed in the present system. This observation suggests that swarming of MTs was favored by moderate interaction between l -DNA and r -DNAs attached to MTs.…”

Section: Discussionsupporting

confidence: 87%

“…Along with the binding interaction of DNA, the driving force of the surface adhered kinesins is another factor that should be considered in the swarming of MTs 32 – 34 . The motility of the r -DNA conjugated MTs was governed by the kinesins which exert force to propel and assemble the MTs into swarms (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

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Control of swarming of molecular robots

Keya

Kabir

Inoue

et al. 2018

Sci Rep

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Recently we demonstrated swarming of a self-propelled biomolecular motor system microtubule (MT)-kinesin where interactions among thousands of motile MTs were regulated in a highly programmable fashion by using DNA as a processor. However, precise control of this potential system is yet to be achieved to optimize the swarm behavior. In this work, we systematically controlled swarming of MTs on kinesin adhered surface by different physicochemical parameters of MT-kinesin and DNA. Tuning the length of DNA sequences swarming was precisely controlled with thermodynamic and kinetic feasibility. In addition, swarming was regulated using different concentration of DNA crosslinkers. Reversibility of swarming was further controlled by changing the concentration of strand displacement DNA signal allowing dissociation of swarm. The control over the swarm was accompanied by variable stiffness of MTs successfully, providing translational and circular motion. Moreover, the morphology of swarm was also found to be changed not only depending on the stiffness but also body length of MTs. Such detail study of precise control of swarming would provide new insights in developing a promising molecular swarm robotic system with desired functions.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 87%

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Control of swarming of molecular robots

Keya

Kabir

Inoue

et al. 2018

Sci Rep

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“…Alongside programming with DNA interaction, different physicochemical parameters of biomolecular motors, DNA, and the surrounding environment can play important roles to control the swarming of MTs, which is required for making sustainable molecular swarm robot systems (Keya et al 2018a). The DNA-based interaction is moderate in strength and specifically controls the interaction between MTs moving in the same direction, unlike the strong streptavidinbiotin crosslinker system, where MTs bind even moving in the opposite direction and results in sticking of MT swarms (Kawamura et al 2011). The binding affinity of DNAconjugated MTs promoting the swarm formation depends on the direction of motion and collision between MTs.…”

Section: Control Of Swarming Of Dna-programmed Biomolecular Enginesmentioning

confidence: 99%

Synchronous operation of biomolecular engines

2020

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Biomolecular motor systems are the smallest natural machines with an ability to convert chemical energy into mechanical work with remarkably high efficiency. Such attractive features enabled biomolecular motors to become classic tools in soft matter research over the past decade. For designing suitably engineered biomimetic systems, the biomolecular motors can potentially be used as molecular engines that can transform energy and ensure great advantages for the construction of bio-nanodevices and molecular robots. From the optimization of their prolonged lifetime to coordinate them into highly complex and ordered structures, enormous efforts have been devoted to make them useful in the synthetic environment. Synchronous operation of the biomolecular engines is one of the key criteria to coordinate them into certain different patterns, which depends on the local interaction of biomolecular motors. Utilizing chemical and physical stimuli, synchronization of biomolecular motor systems has become possible, which allows them to coordinate into different higher ordered patterns with different modes of functionality. Recently, programmed synchronous operation of the biomolecular engines has also been demonstrated, using a smart biomaterial to build up swarms reminiscent of nature. Here, we review the recent progress in the synchronized operation of biomolecular motors in engineered systems to explicitly program their interaction and further their applications. Such developments in the coordination of biomolecular motors have opened a broad way to explore the construction of future autonomous molecular machines and robots based on synchronization of biomolecular engines.

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“…An example procedure for the motility assay after obtaining the materials of RMT and frozen kinesin-GFP is described below. 16) A pair of coverslips with a pair strip of double sided-sticky tape (spacer) is used as an observation container for assembling a flow-cell (Fig. 3).…”

Section: Introductionmentioning

confidence: 99%

Integration of nanometric motor proteins towards a macroscopic power tool

Kawamura

2023

Jpn. J. Appl. Phys.

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The use of motor proteins as power nanotools on a small scale as a single-molecular driving unit and on a larger scale via integration has been attempted considering that successful cases of such integrations exist in natural systems such as in the organs of the animal body. However, it is challenging to replicate such integrations in artificial systems, and therefore, several studies focus on harnessing the movements of individual motor proteins in nanotechnology. In this paper, existing literature is reviewed to discuss the integration of motor proteins to scale up and to accelerate the movements in complex systems. A method that focuses on kinesin-microtubule motor proteins is introduced to help replicate the movements of motor proteins and allow researchers from different fields to gain interest in this topic; the potential applications of the motor proteins and the needs of orientation toward concrete issue for future are also noted.

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Formation of motile assembly of microtubules driven by kinesins

Cited by 6 publications

References 55 publications

Control of swarming of molecular robots

Control of swarming of molecular robots

Synchronous operation of biomolecular engines

Integration of nanometric motor proteins towards a macroscopic power tool

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