Formation of Multinucleated Giant Cells In Vitro Is Dependent on the Stage of Monocyte to Macrophage Maturation

Möst, Johannes; Spötl, Ludwig; Mayr, Gertraud; Gasser, Annette; Sarti, Alessandra; Dierich, Manfred P.

doi:10.1182/blood.v89.2.662

Cited by 73 publications

(22 citation statements)

References 46 publications

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“…Clanchy et al [20] have shown that human blood-derived osteoclasts form with high frequencies from proliferating, immature monocytes. Multinucleated giant cells also form at higher frequencies from fresh monocytes than from monocytes differentiated for some days toward macrophages [21]. Although osteoclasts can be generated from all three stages of myeloid differentiation in mouse bone marrow, our study revealed that myeloid blasts rather than monocytes are the candidate cells from which osteoclasts are readily formed.…”

Section: Discussionmentioning

confidence: 59%

Myeloid blasts are the mouse bone marrow cells prone to differentiate into osteoclasts

Vries

Schoenmaker

Hooibrink

et al. 2009

Journal of Leukocyte Biology

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Cells of the myeloid lineage at various stages of maturity can differentiate into multinucleated osteoclasts. Yet, it is unclear which developmental stages of this lineage are more prone to become osteoclasts than others. We investigated the osteoclastogenic potential of three successive stages of myeloid development isolated from mouse bone marrow. Early blasts (CD31hi/Ly-6C-), myeloid blasts (CD31+/Ly-6C+), and monocytes (CD31-/Ly-6Chi), as well as unfractionated marrow cells, were cultured in the presence of M-CSF and receptor activator of NF-B ligand (RANKL), and the differentiation toward multinucleated cells and their capacity to resorb bone was assessed. Myeloid blasts developed rapidly into multinucleated cells; in only 4 days, maximal numbers were reached, whereas the other fractions required 8 days to reach maximal numbers. Bone resorption was observed after 6 (myeloid blasts and monocyte-derived osteoclasts) and 8 (early blast-derived osteoclasts) days. This difference in kinetics in osteoclast-forming capacity was confirmed by the analysis of osteoclast-related genes. In addition, the myeloid blast fraction proved to be most sensitive to M-CSF and RANKL, as assessed with a colony-forming assay. Our results show that osteoclasts can develop from all stages of myeloid differentiation, but myeloid blasts are equipped to do so within a short period of time.

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Section: Discussionmentioning

confidence: 59%

Myeloid blasts are the mouse bone marrow cells prone to differentiate into osteoclasts

Vries

Schoenmaker

Hooibrink

et al. 2009

Journal of Leukocyte Biology

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“…Multinucleated giant cell formation is known to be linked to chronic immune responses, particularly to indigestible materials or chronic infections such as tuberculosis. 17 It has been demonstrated previously, in vitro and in vivo, that a strong inverse relationship exists between the rate of material degradation and the degree of inflammatory response to implanted material: A fast resorption process is accompanied by a high degree of inflammation. 13 Furthermore, it has been demonstrated that polylactic acid-based devices implanted subcutaneously in rats triggered an inflammatory response, including multinu- Figure 3.…”

Section: Discussionmentioning

confidence: 97%

Coronary Artery Vessel Healing Pattern, Short and Long Term, After Implantation of the Everolimus‐Eluting Bioresorbable Vascular Scaffold

Kraak

Boer

Elias

et al. 2015

JAHA

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BackgroundAlthough the Absorb bioresorbable vascular scaffold is increasingly used in daily clinical practice for the treatment of coronary artery disease, the exact vascular healing pattern and the resorption process in humans is unknown because histological data are derived only from animal studies.Methods and ResultsWe have obtained 4 autopsies (5 scaffolds) since August 2013. Duration of bioresorbable vascular scaffold implantation ranged from 3 to 501 days. All autopsies and histological assessments were performed by dedicated cardiovascular pathologists. At 1 week after bioresorbable vascular scaffold implantation, struts were covered with a fine layer of fibrin and platelets. At 113 days, the scaffold struts were fully covered with smooth muscle cells. Hyaline eosinophilic and proteoglycan material infiltrating the scaffold struts was observed at 501 days after implantation. At all time points, we observed the presence of multinuclear foreign body giant cells adjacent to the scaffold struts.ConclusionsResorption and healing processes after bioresorbable vascular scaffold implantation in human patients mirror those observed in porcine models. The presence of multinucleated foreign body giant cells at both short‐ and long‐term follow‐up needs further investigation and may be related to a low‐grade absorptive inflammatory response to the polymer.

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“…Cell harvesting: Monocytes from healthy donors were harvested as described previously [23]. Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood of healthy adult volunteers by density gradient centrifugation with Ficoll--Paque.…”

Section: Cell Migrution Ussaymentioning

confidence: 99%

Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone

Liao

Yurgelun

et al. 2005

Journal Orthopaedic Research

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Giant cell tumor (GCT) of bone is a unique bone lesion that is characterized by an excessive number of multinucleated osteoclasts. GCT consists of neoplastic stromal cells, multinucleated osteoclasts and their precursors, thus serving as a naturally occurring human disease model for the study of osteoclastogenesis. It still remains unclear how stromal cells of GCT recruit osteoclast precursors. In the present study, we characterized the cellular components of GCT and confirmed the presence of C D 14+-monocytes/CD68'-macrophages and CD34+-heniatopoetic stem cells that express CXCR4, a specific receptor for SDF-1; SDF-1 gene expression and presence of SDF-1 protein were confirmed by real time RT-PCR, in situ hybridization, and immunohistochemistry in the GCT tissue and cultured cells. SDF-1 was present at 25-50ng/ml in the conditioned media from the GCT cultures, which is in the range of physiological chemotactic concentration. Migration of osteoclast precursors was 2.5-fold higher in response to GCT conditioned media compared to the control media; and migration was inhibited by an average of 36% with anti-SDF-1 neutralizing antibody or competing recombinant SDF-1. These results suggest that SDF-1 is one of the significant chemoattractant factors involved in the recruitment of hematopoietic osteoclast precursor cells during tumor-induced osteoclastogenesis.

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Formation of Multinucleated Giant Cells In Vitro Is Dependent on the Stage of Monocyte to Macrophage Maturation

Cited by 73 publications

References 46 publications

Myeloid blasts are the mouse bone marrow cells prone to differentiate into osteoclasts

Myeloid blasts are the mouse bone marrow cells prone to differentiate into osteoclasts

Coronary Artery Vessel Healing Pattern, Short and Long Term, After Implantation of the Everolimus‐Eluting Bioresorbable Vascular Scaffold

Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone

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