Formation of Multiple Structural Formats of DNA in a Bio-Deep Eutectic Solvent

Mukesh, Chandrakant; Prasad, Kamalesh

doi:10.1002/macp.201500009

Cited by 16 publications

(14 citation statements)

References 32 publications

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“…Furthermore, the condensation of the DNA structure was earlier observed by us in a deep eutectic solvent. 36 Moreover, the polyplexes formed inside ILs showed stability in water up to a certain degree, such as the stable polyplexes did not precipitate out in any ILs upon the addition of water up to IL: water ratio of 1:8 in 2-HEAA, 1:10 in 2-HEAF, 1:1.5 in Ch-Ac, and 1:3 in Ch-For. However, the addition of a little bit of water beyond the above ratio resulted in precipitation, as shown in Figure 2.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

Ionic Liquid-Mediated Preparation of Noncytotoxic Hemocompatible Stable DNA−ε-Poly-l-lysine Polyplexes: A New Sustainable Approach for the Bulk Production of Potential Nonviral Vectors for Gene Delivery Applications

Bhatt

Sequeira

Vohra

et al. 2020

ACS Sustainable Chem. Eng.

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Nonviral vectors are gaining importance as gene delivery systems. However, to meet the large-scale applications, their sustainable production is of utmost importance. Hence, a cost-effective and one-pot strategy for their bulk production is desirable. Herein, a sustainable method for the facile production of nonviral gene vectors, using ε-poly-l-lysine and DNA, having the potential for gene delivery applications was developed using ionic liquids. The hemocompatible nanosized negatively charged polyplexes thus prepared were stable in aqueous media and were found to be noncytotoxic against human HeLa cells. The method developed produces no byproducts and, hence, has maximum atom economy, which is essential while considering it for bulk production. Further, the process is a single step and facilitated at room temperature (30 °C), which makes it energy efficient and cost-effective. Because of the ease of production, the method for the production of polyplexes reported herein may promote the bulk production of biopolymer-based nonviral vectors for advanced gene delivery applications.

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Section: ■ Results and Discussionmentioning

confidence: 97%

Ionic Liquid-Mediated Preparation of Noncytotoxic Hemocompatible Stable DNA−ε-Poly-l-lysine Polyplexes: A New Sustainable Approach for the Bulk Production of Potential Nonviral Vectors for Gene Delivery Applications

Bhatt

Sequeira

Vohra

et al. 2020

ACS Sustainable Chem. Eng.

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“…25 Self-assembled DNA-based microgels were also found in ethylene glycol DES. 26 Self-assembly without the presence of amphiphiles has also been demonstrated. 27 This study used dynamic light scattering and small-angle X-ray scattering (SAXS) to demonstrate the presence of the aggregates in the monophasic region of ternary eutectic mixtures without surfactants.…”

Section: Introductionmentioning

confidence: 99%

Micelle structure in a deep eutectic solvent: a small-angle scattering study

Sanchez-Fernandez

Edler

Arnold

et al. 2016

Phys. Chem. Chem. Phys.

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In recent years many studies into green solvents have been undertaken and deep eutectic solvents (DES) have emerged as sustainable and green alternatives to conventional solvents since they may be formed from cheap non-toxic organic precursors. In this study we examine amphiphile behaviour in these novel media to test our understanding of amphiphile self-assembly within environments that have an intermediate polarity between polar and non-polar extremes. We have built on our recently published results to present a more detailed structural characterisation of micelles of sodium dodecylsulfate (SDS) within the eutectic mixture of choline chloride and urea. Here we show that SDS adopts an unusual cylindrical aggregate morphology, unlike that seen in water and other polar solvents. A new morphology transition to shorter aggregates was found with increasing concentration. The self-assembly of SDS was also investigated in the presence of water; which promotes the formation of shorter aggregates.

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“…Mukesh and Prasad reported for the first time the preparation of three different structural formats of DNA in a single DES, specifically ChCl/Ethylene glycol (1:2). 35 Panzer and coworkers presented a new class of gelatin-based gels featuring ChCl/Ethylene glycol (1:2). 36 These ionic soft materials were synthesized in a facile manner using the thermally modulated coil-helix transition process of gelatin induced by heating and subsequently cooling the gelatin/DES mixture.…”

Section: Types Of Gelatorsmentioning

confidence: 99%

“…In this case, the gel materials show different behaviors upon changes in the pH of the system. For instance, Mukesh and Prasad, 35 demonstrated the preparation of DNA-based ionic gels, in which the formation of three different morphologies of DNA were obtained in the presence of ChCl/Ethylene glycol (1:2). The DNA was converted into pH reversible ionic gels, comprising aggregated DNA structures (pH 2.98), spheroid shaped DNA microstructured assemblies, and pH responsive DNA micro-hydrogels (pH 7.30).…”

Section: Physico-chemical Properties Of Ionic Gels Based On Dessmentioning

confidence: 99%

Emerging Ionic Soft Materials Based on Deep Eutectic Solvents

2020

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In the last five years, the use of deep eutectic solvents (DESs) have been opening new perspectives towards the creation of novel ionic soft materials as alternatives to expensive ionic liquids. This Mini-Review highlights the progress and advances in soft ionic materials or gels, mostly composed by a DES immobilized within difference matrices, such as linear polymers, polymer networks, biopolymers, supramolecular compounds or sol-gel derived silica networks. By taking advantage of the DESs characteristics and properties in the solid state, this building system delivers a variety of tailor-made materials showing different functionalities (ionic conductivity, self-healing, stretchability and pH-responsiveness) and offers a way to circumvent drawbacks related to shaping and risk of leakage in many technological applications. In this context, we provide a judicious analysis of these emerging ionic soft materials, their properties and applications open in energy, (bio)electronics, drug delivery, analytical chemistry, and wastewater treatment. Perspectives and opportunities for future research directions on this blossoming field are also discussed.

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Formation of Multiple Structural Formats of DNA in a Bio-Deep Eutectic Solvent

Cited by 16 publications

References 32 publications

Ionic Liquid-Mediated Preparation of Noncytotoxic Hemocompatible Stable DNA−ε-Poly-l-lysine Polyplexes: A New Sustainable Approach for the Bulk Production of Potential Nonviral Vectors for Gene Delivery Applications

Ionic Liquid-Mediated Preparation of Noncytotoxic Hemocompatible Stable DNA−ε-Poly-l-lysine Polyplexes: A New Sustainable Approach for the Bulk Production of Potential Nonviral Vectors for Gene Delivery Applications

Micelle structure in a deep eutectic solvent: a small-angle scattering study

Emerging Ionic Soft Materials Based on Deep Eutectic Solvents

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