Formation of nexuses and electrotonic transmission between myocardial and FL cells in monolayer culture

Goshima, Kiyota

doi:10.1016/0014-4827(70)90339-3

Cited by 93 publications

(31 citation statements)

References 29 publications

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“…1981 a). Goshima (1970) demonstrated that two myocardial cells could ACTION POTENTIAL S YNCHRON Y be coupled across non-excitable cells (HeLa, FL) and that nexus were present. Thus, support is lent to the suggestion that electrically active cells may be widely coupled across non-active cells which are still functionally developing in the early phases of cardiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Action potential synchrony in embryonic precontractile chick heart: optical monitoring with potentiometric dyes.

Fujii¹,

Hirota²,

Kamino³

1981

The Journal of Physiology

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SUMMARY1. Using an optical method for monitoring membrane potential, we recorded spontaneous action potentials simultaneously in several different areas of the 7-9 somite embryonic chick hearts.2. Absorption signals resembling spontaneous action potentials were well synchronized among the different areas in the preheating embryonic heart during the 7-9 somite stages of development, and the synchronization spread over the entire area of the heart. From these experimental results, it is evident that there is electrical coupling among embryonic chick heart cells even in the early stages of cardiogenesis.3. When an embryonic heart was separated into right and left or anterior and posterior parts, the action potential synchrony between the two halves was completely blocked; however, the synchrony and the intrinsic rhythmicity in action potential recurrence remained in each part.

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Section: Discussionmentioning

confidence: 99%

Action potential synchrony in embryonic precontractile chick heart: optical monitoring with potentiometric dyes.

Fujii¹,

Hirota²,

Kamino³

1981

The Journal of Physiology

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“…Recently, however, Spira (48) described 30-50 A gap junctions in all but the deeper layers of embryonic chick myocardial cells from stage 7 (24 hr) to stage 30 (7 days) . Nexuses were also shown in tissue culture between mammalian cardiac myoblasts (5,49), fibroblasts (5), and smooth muscle cells (50,51) . DeHaan and Hirakow (17) reported that small punctate regions of close membrane apposition formed between isolated heart cells within minutes after they established contact .…”

Section: Ultrastructure Of the Strandmentioning

confidence: 99%

Synthetic Strands of Cardiac Muscle

Purdy

Lieberman

Roggeveen

et al. 1972

The Journal of Cell Biology

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Spontaneously active bundles of cardiac muscle (synthetic strands) were prepared from isolated cells of 11-13-day old embryonic chick hearts which were disaggregated with trypsin . Linear orientation of the cells was obtained by plating them on agar-coated culture dishes in which either grooves were cut in the agar film or a thin line of palladium was deposited over the agar . The influence of cell-to-cell and cell-to-substrate interactions was observed with time lapse cinematography and the formation of the synthetic strand was shown to involve both random and guided cell movements, enlargement of aggregates by accretion and coalescence, and the compact linear arrangement of cells along paths of preferential adhesion. Electron microscope investigations of these strands showed that a dispersed population of heart cells organized into an inner core of muscle cells and an outer sheath of fibroblast-like cells . The muscle cells contained well-developed, but widely spaced myofibrils, a developing sarcoplasmic reticulum associated in part with the myofibrils and in part with the sarcolemma, an abundance of nonmembrane bound ribosomes and glycogen, and a prominent Golgi complex. Numerous specialized contacts were observed between the muscle cells in the strand, e.g., fasciae adherentes, desmosomes, and nexuses . A distinct type of muscle cell characterized by its pale appearance was regularly observed in the strand and was noted to be similar to Purkinje cells described in the adult avian conduction system and in developing chick myocardium . The present findings were compared with other observations of the developing myocardium, in situ, and it was concluded that, by a number of criteria, the muscle cells of the strand were differentiating normally and suitably organized for electrophysiological studies .

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“…Although the main role of fibroblasts, consisting of synthesis and maintenance of the mechanical scaffold for cardiomyocytes, is undisputed, their involvement in electrophysiological processes within the working myocardium is less clear. Electrical coupling between cardiomyocytes and fibroblasts was demonstrated several decades ago in cell cultures (7) and, more recently, in intact tissue (8). Fibroblasts are coupled with gap junction channels containing Cx43.…”

Section: Introductionmentioning

confidence: 99%

Ischemic Preconditioning Protects Against Gap Junctional Uncoupling in Cardiac Myofibroblasts

Sundset

Cooper

Mikalsen

et al. 2004

Cell Communication & Adhesion

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Formation of nexuses and electrotonic transmission between myocardial and FL cells in monolayer culture

Cited by 93 publications

References 29 publications

Action potential synchrony in embryonic precontractile chick heart: optical monitoring with potentiometric dyes.

Action potential synchrony in embryonic precontractile chick heart: optical monitoring with potentiometric dyes.

Synthetic Strands of Cardiac Muscle

Ischemic Preconditioning Protects Against Gap Junctional Uncoupling in Cardiac Myofibroblasts

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