Formation of Polysulfides in an Oxygen Rich Freshwater Lake and Their Role in the Production of Volatile Sulfur Compounds in Aquatic Systems

Gun, Jenny; Goifman, A.; Shkrob, Ilia; Kamyshny, Alexander; Гинзбург, Б. М.; Hadas, Ora; Dor, Inka; Модестов, А. Д.; Lev, Ovadia

doi:10.1021/es991389x

Cited by 59 publications

(37 citation statements)

References 25 publications

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“…As regard to recoveries, DMTS is by far the compound with the best recovery value (98%) and DMDS value is 50%.The recoveries of these compounds are similar to the ones reported (Gun et al, 2000). DMDSe recovery is low, but having low detection limits provides good detection.…”

Section: Quality Parameters Of the Methodssupporting

confidence: 76%

Determination of dimethyl selenide and dimethyl sulphide compounds causing off-flavours in bottled mineral waters

et al. 2016

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Section: Quality Parameters Of the Methodssupporting

confidence: 76%

Determination of dimethyl selenide and dimethyl sulphide compounds causing off-flavours in bottled mineral waters

et al. 2016

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“…Cyclic voltammetry (CV) was used to detect and to quantify soluble and electroactive compounds in batch cultures of A. sulfurireducens, namely, polysulfides (primarily H 2 S 5 at acidic pH [41]; pK1 for H 2 S 5 is 3.5 at 25°C, for example [42]), nanocrystalline S 8 0 , and hydrogen sulfide (H 2 S). Standard additions of Na 2 S and Na 2 S 5 solutions were performed in PS medium with the pH adjusted to both 3.0 and 4.0.…”

Section: Resultsmentioning

confidence: 99%

Involvement of Intermediate Sulfur Species in Biological Reduction of Elemental Sulfur under Acidic, Hydrothermal Conditions

Boyd

Druschel

2013

Appl Environ Microbiol

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The thermoacidophile and obligate elemental sulfur (S 8 0 )-reducing anaerobe Acidilobus sulfurireducens 18D70 does not associate with bulk solid-phase sulfur during S 8 0 -dependent batch culture growth. Cyclic voltammetry indicated the production of hydrogen sulfide (H 2 S) as well as polysulfides after 1 day of batch growth of the organism at pH 3.0 and 81°C. The production of polysulfide is likely due to the abiotic reaction between S 8 0 and the biologically produced H 2 S, as evinced by a rapid cessation of polysulfide formation when the growth temperature was decreased, inhibiting the biological production of sulfide. After an additional 5 days of growth, nanoparticulate S 8 0 was detected in the cultivation medium, a result of the hydrolysis of polysulfides in acidic medium. To examine whether soluble polysulfides and/or nanoparticulate S 8 0 can serve as terminal electron acceptors (TEA) supporting the growth of A. sulfurireducens, total sulfide concentration and cell density were monitored in batch cultures with S 8 0 provided as a solid phase in the medium or with S 8 0 sequestered in dialysis tubing. The rates of sulfide production in 7-day-old cultures with S 8 0 sequestered in dialysis tubing with pore sizes of 12 to 14 kDa and 6 to 8 kDa were 55% and 22%, respectively, of that of cultures with S 8 0 provided as a solid phase in the medium. These results indicate that the TEA existed in a range of particle sizes that affected its ability to diffuse through dialysis tubing of different pore sizes. Dynamic light scattering revealed that S 8 0 particles generated through polysulfide rapidly grew in size, a rate which was influenced by the pH of the medium and the presence of organic carbon. Thus, S 8 0 particles formed through abiological hydrolysis of polysulfide under acidic conditions appeared to serve as a growth-promoting TEA for A. sulfurireducens.

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“…It is also unlikely that epilimnetic organisms would suffer from strong oxidative stress, because they frequently experience high oxygen concentration and have multiple mechanisms to cope with it (22). Although conversion of methylated compounds such as methanethiol to methane can be bioenergetically favorable for methanogenic Archaea (13), neither methanethiol nor other methylated compounds are commonly found in high concentrations in oxygenated epilimnion in lakes (23)(24)(25). In our experiments, the addition of neither inorganic phosphate nor methylated compounds affected methane production; hence, pelagic methanogenesis in Lake Stechlin did not seem to depend on phosphate or methylated substrates like other systems (12,13).…”

Section: Discussionmentioning

confidence: 99%

Microbial methane production in oxygenated water column of an oligotrophic lake

Grossart

Frindte

Dziallas

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

289

328

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The prevailing paradigm in aquatic science is that microbial methanogenesis happens primarily in anoxic environments. Here, we used multiple complementary approaches to show that microbial methane production could and did occur in the well-oxygenated water column of an oligotrophic lake (Lake Stechlin, Germany). Oversaturation of methane was repeatedly recorded in the well-oxygenated upper 10 m of the water column, and the methane maxima coincided with oxygen oversaturation at 6 m. Laboratory incubations of unamended epilimnetic lake water and inoculations of photoautotrophs with a lake-enrichment culture both led to methane production even in the presence of oxygen, and the production was not affected by the addition of inorganic phosphate or methylated compounds. Methane production was also detected by in-lake incubations of lake water, and the highest production rate was 1.8-2.4 nM·h −1 at 6 m, which could explain 33-44% of the observed ambient methane accumulation in the same month. Temporal and spatial uncoupling between methanogenesis and methanotrophy was supported by field and laboratory measurements, which also helped explain the oversaturation of methane in the upper water column. Potentially methanogenic Archaea were detected in situ in the oxygenated, methane-rich epilimnion, and their attachment to photoautotrophs might allow for anaerobic growth and direct transfer of substrates for methane production. Specific PCR on mRNA of the methyl coenzyme M reductase A gene revealed active methanogenesis. Microbial methane production in oxygenated water represents a hitherto overlooked source of methane and can be important for carbon cycling in the aquatic environments and water to air methane flux. epilimnic methane peak | methanogens A lthough methane makes up <2 parts per million by volume (ppmv) of the atmosphere, it accounts for 20% of the total radiative forcing among all long-lived greenhouse gases (1). In the aquatic environments, methane is also an important substrate for microbial production (2). The prevailing paradigm is that microbial methanogenesis occurs primarily in anoxic environments (3, 4). A commonly observed paradox is methane accumulation in well-oxygenated waters (2, 5), which is often assumed to be the result of physical transport from anoxic sediment and water (6-8) or in situ production within microanoxic zones (9-11). Two recent studies challenged this paradigm and suggested that microbes in oligotrophic ocean can metabolize methylated compounds and release methane even aerobically (12, 13). These claims are not without caveats, because the amounts of methylated compounds added [1-10 μM methylphosphonate (12) and 50 μM dimethyl sulfoniopropionate (13)] were far higher than their environmental concentrations, and therefore, the ecological relevance remains obscure. Moreover, dissolved oxygen (DO) was not monitored during the long incubation (5-6 d), and the possibility that the experimental setups had become anoxic before methane production could not be dismissed.* Despite the unce...

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Formation of Polysulfides in an Oxygen Rich Freshwater Lake and Their Role in the Production of Volatile Sulfur Compounds in Aquatic Systems

Cited by 59 publications

References 25 publications

Determination of dimethyl selenide and dimethyl sulphide compounds causing off-flavours in bottled mineral waters

Determination of dimethyl selenide and dimethyl sulphide compounds causing off-flavours in bottled mineral waters

Involvement of Intermediate Sulfur Species in Biological Reduction of Elemental Sulfur under Acidic, Hydrothermal Conditions

Microbial methane production in oxygenated water column of an oligotrophic lake

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