Formation of pre-metastatic niches induced by tumor extracellular vesicles in lung metastasis

Liu, Yuping; Mao, Dengxuan; Wang, Hong; Che, Xiaoyu; Chen, Yan

doi:10.1016/j.phrs.2023.106669

Cited by 13 publications

(8 citation statements)

References 166 publications

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“…In particular, the lungs exhibited severe vascular endothelial glycocalyx shedding. This is consistent with the fact that many types of cancer metastasize to the lungs more than to other tissues [ 36 , 62 ]. Tumor metastasis is not only related to glycocalyx shedding but also to extracellular vesicles and soluble factors produced by orthotopic tumors.…”

Section: Discussionsupporting

confidence: 85%

“…As the lung is the major site of metastasis for many types of cancer [ [34] , [35] , [36] ], we excised lungs from mice at 24 h after injection of conditioned medium, after which they were washed with 0.9% physiological saline, sectioned, and fixed for at least 2 h at 4 °C with electron microscopy fixative containing 2% lanthanum nitrate (47.50 mL 0.2 mol/L sodium cacodylate, 3.99 mL 0.2 mol/L HCl, purified water 138.51 mL, 10 mL 50% (v/v) glutaraldehyde, and 5.33 g lanthanum nitrate) to label the vascular endothelial glycocalyx. The samples were rinsed three times (15 min each) with 0.1 M phosphoric acid buffer (pH 7.0), fixed with 1% osmic acid solution for 2–4 h, rinsed again three times with 0.1 M phosphoric acid buffer, dehydrated with a graded ethanol series, treated with 100% ethanol for 20 min, and finally transferred to pure acetone for 20 min.…”

Section: Methodsmentioning

confidence: 99%

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Destruction of vascular endothelial glycocalyx during formation of pre-metastatic niches

Qu,

Du,

et al. 2024

Heliyon

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Section: Discussionsupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Destruction of vascular endothelial glycocalyx during formation of pre-metastatic niches

Qu,

Du,

et al. 2024

Heliyon

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“…The lung is the most common site of malignant tumor metastasis, such as breast cancer 20,21 and hepatocellular carcinoma 32 . When lung metastasis is discovered by X‐ray or CT, the patients are usually at an advanced stage and have a poor prognosis.…”

Section: Discussionmentioning

confidence: 99%

“…The lung is the most common site of malignant tumor metastasis, such as breast cancer 20 , 21 and hepatocellular carcinoma. 32 When lung metastasis is discovered by X‐ray or CT, the patients are usually at an advanced stage and have a poor prognosis. For breast cancer patients with lung metastasis, the prognosis is exceedingly poor with a median survival of only 25 months.…”

Section: Discussionmentioning

confidence: 99%

Effect of lung metastasis on the treatment and prognosis of patients with gestational trophoblastic neoplasia: A systematic review and meta‐analysis

Zhang,

Guo,

et al. 2024

Acta Obstet Gynecol Scand

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IntroductionGestational trophoblastic neoplasia (GTN) is a highly invasive tumor, mainly spreading to the lungs. However, lung metastasis in GTN is usually not considered as an adverse prognostic factor. Therefore, the aim of this study was to summarize the results of previous studies and evaluate the effects of lung metastasis on the treatment and prognosis of GTN.Material and methodsThe study was prospectively registered in PROSPERO (CRD42023372371). Electronic databases including PubMed, Embase, the Cochrane Library, Chinese National Knowledge Infrastructure, Wanfang, and China Biomedical Literature Database were used for a systematical search of relevant studies published up to November 21, 2022. The observational studies reporting the clinical outcomes of GTN patients with and without lung metastasis were selected. The incidences of resistance, relapse, and mortality of GTN patients were extracted and successively grouped based on the presence of lung metastasis. The pooled relative risks (RRs) and 95% confidence interval (95% CI) of the eligible studies were calculated. The qualities of included studies were assessed with the Newcastle‐Ottawa Scale and the certainty of evidence was graded based on the GRADE. The meta‐analysis was performed using Stata 12.0 and GradePro software.ResultsFive publications with 3629 GTN patients were included. The meta‐analysis revealed that the GTN with lung metastasis was strongly correlated with first‐line chemoresistance (pooled RR = 1.40, 95% CI: 1.22 to 1.61, p < 0.001), recurrence (pooled RR = 3.03, 95% CI: 1.21 to 7.62, p = 0.018), and disease‐specific death (pooled RR = 22.11, 95% CI: 3.37 to 145.08, p = 0.001). Ethnicity was also an important factor and Caucasian GTN patients with lung metastasis showed a higher risk of recurrence as revealed by the subgroup analysis (pooled RR = 5.10, 95% CI: 2.38 to 10.94, p < 0.001).ConclusionsGTN patients with lung metastasis exhibited a higher risk of chemoresistance, relapse, and disease‐specific death. Patients with lung metastasis among the Caucasian population had a higher risk of recurrence than Asian populations. Therefore, the presence of lung metastases might be considered as a high‐risk factor for prognosis of GTN and deserves more attention in the choice of first‐line chemotherapy regimens and follow‐up.

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“…In fact, it is estimated that 60–70% of patients who die of breast cancer have lung metastasis [ 10 ]. In particular, the crosstalk between primary tumor and early formation of metastatic niches might be mediated by extracellular vesicles and tumor/stroma-derived factors [ 11 ]. EVs are membrane-enclosed vesicles released by all types of cells, and they carry various types of molecules, such as miRNA, proteins, lipids, and other bioactive factors.…”

Section: Introductionmentioning

confidence: 99%

Triple-Negative Breast Cancer EVs Modulate Growth and Migration of Normal Epithelial Lung Cells

Leone,

Santoro,

Soricelli

et al. 2024

IJMS

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Breast cancer is the most common cancer amongst women worldwide. Recently, owing to screening programs and new technologies, the survival rate has increased significantly. Breast cancer can potentially develop metastases, and, despite them, lung metastases generally occur within five years of breast cancer diagnosis. In this study, the objective was to analyze the effect of breast cancer-derived EVs on a lung epithelial cell line. BEAS-2B cells were treated with extracellular vesicles (EVs) derived from triple-negative breast cancer cells (TNBCs), e.g., MDA-MB-231 and HS578T, separated using differential ultracentrifugation. We observed an increased growth, migration, and invasiveness of normal epithelial lung cells over time in the presence of TNBC EVs compared to the control. Therefore, these data suggest that EVs released by tumor cells contain biological molecules capable of influencing the pro-tumorigenic activity of normal cells. Exploring the role of EVs in oncology research and their potential cargo may be novel biomarkers for early cancer detection and further diagnosis.

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Formation of pre-metastatic niches induced by tumor extracellular vesicles in lung metastasis

Cited by 13 publications

References 166 publications

Destruction of vascular endothelial glycocalyx during formation of pre-metastatic niches

Destruction of vascular endothelial glycocalyx during formation of pre-metastatic niches

Effect of lung metastasis on the treatment and prognosis of patients with gestational trophoblastic neoplasia: A systematic review and meta‐analysis

Triple-Negative Breast Cancer EVs Modulate Growth and Migration of Normal Epithelial Lung Cells

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