Formation of reactive oxygen species by irradiation of cold atmospheric pressure plasma jet to water depends on the irradiation distance

Anzai, Kazunori; Aoki, Tamami; Koshimizu, Satoko; Takaya, Reina; Tsuchida, Kazunori; Takajo, Tokuko

doi:10.3164/jcbn.18-102

Cited by 19 publications

(15 citation statements)

References 23 publications

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“…In fact, in the presence of 1 mM ascorbic acid as a reducing reagent, virtually no HSQC spectral changes were observed for a CAP-treated Aβ (1-40) solution ( Figure S1d). Various reactive oxygen species, including H 2 O 2 , are supposed to be produced upon water irradiation with CAP [21,22]. In the present study, we confirmed that H 2 O 2 is generated by CAP irradiation in the buffer solution ( Figures S4 and S5).…”

Section: Reactive Species Responsible For Methionine Oxidation Of Aβsupporting

confidence: 84%

Cold Atmospheric Plasma Modification of Amyloid β

Yagi-Utsumi

Tanaka

Otsubo

et al. 2021

IJMS

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Cold atmospheric plasma (CAP) has attracted much attention in the fields of biotechnology and medicine owing to its potential utility in clinical applications. Recently accumulating evidence has demonstrated that CAP influences protein structures. However, there remain open questions regarding the molecular mechanisms behind the CAP-induced structural perturbations of biomacromolecules. Here, we investigated the potential effects of CAP irradiation of amyloid β (Aβ), an amyloidogenic protein associated with Alzheimer’s disease. Using nuclear magnetic resonance spectroscopy, we observed gradual spectral changes in Aβ after a 10 s CAP pretreatment, which also suppressed its fibril formation, as revealed by thioflavin T assay. As per mass spectrometric analyses, these effects were attributed to selective oxidation of the methionine residue (Met) at position 35. Interestingly, this modification occurred when Aβ was dissolved into a pre-irradiated buffer, indicating that some reactive species oxidize the Met residue. Our results strongly suggest that the H2O2 generated in the solution by CAP irradiation is responsible for Met oxidation, which inhibits Aβ amyloid formation. The findings of the present study provide fundamental insights into plasma biology, giving clues for developing novel applications of CAP.

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Section: Reactive Species Responsible For Methionine Oxidation Of Aβsupporting

confidence: 84%

Cold Atmospheric Plasma Modification of Amyloid β

Yagi-Utsumi

Tanaka

Otsubo

et al. 2021

IJMS

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“…We further measure the radial and axial spreading of the green emission in the ambient air by imaging and highly resolved OES techniques. This yields a measure of plasma volume which is an important parameter for a variety of applications 17 . Small size of APPJs often limit their use to small scale processes and materials treatment 18 .…”

Section: Resultsmentioning

confidence: 99%

A KHz frequency cold atmospheric pressure argon plasma jet for the emission of O(1S) auroral lines in ambient air

Jaiswal

Aguirre

Prakash

2021

Sci Rep

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Creation of the “auroral” green line, a fascinating occurrence commonly observed in the upper atmosphere, has long been a difficult endeavor, especially at atmospheric pressure. Here we report strong emission of the “auroral” green line for the first time in a kHz frequency, linear field atmospheric pressure plasma jet system. The device used 99.999% pure argon as a working gas for the plasma generation. Optical emission spectroscopy measurements of the after discharge region show the existence of 557.7 nm emission which corresponds to the transition O($$^1$$ 1 S)–O($$^1$$ 1 D). The intensity of the produced green line is strong enough that the entire plasma plume in the ambient air is visible as a green plasma. We provide the chemical reactions of O($$^1$$ 1 S) production in the plasma and the estimation of the density of the O($$^1$$ 1 S) metastable state using the kinetic reactions. Further, the O($$^1$$ 1 S) emission is characterized by changing the flow rate of argon, applied voltage and electrode gap. The adequate plasma length ($$> 25 \, \hbox {mm}$$ > 25 mm ) along with the production of a variety of reactive components viz; OH, $${\hbox {N}}_{2}^{+}$$ N 2 + and oxygen (777 nm) make this configuration useful for applications such as: blood coagulation, cancer treatment, sterilization, and waste treatment. Moreover, this setup can be potentially used as a test bed for the in-depth understanding of plasma chemistry relevant to the aurora and comet tails using a laboratory setting.

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“…NTP is effective for regenerating damaged tissue epithelium and thus protects normal tissue in various disease models [7,9,10,28]. This suggests that the use of NTP as an alternative therapy can overcome the In particular, NTPAM is useful because it has an anticancer effect similar to that of existing NTP and can be directly applied to tumors [29][30][31]. The anticancer effects of NTP are broadly related to mechanisms such as apoptosis induction through ROS generation and cytoskeletal modulation [6,31,32], but the detailed underlying mechanisms are unclear.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that the use of NTP as an alternative therapy can overcome the In particular, NTPAM is useful because it has an anticancer effect similar to that of existing NTP and can be directly applied to tumors [29][30][31]. The anticancer effects of NTP are broadly related to mechanisms such as apoptosis induction through ROS generation and cytoskeletal modulation [6,31,32], but the detailed underlying mechanisms are unclear. To our knowledge, there have been few studies concerning the mechanism by which NTPAM mediates its anticancer effects.…”

Section: Discussionmentioning

confidence: 99%

Head and Neck Cancer Cell Death due to Mitochondrial Damage Induced by Reactive Oxygen Species from Nonthermal Plasma‐Activated Media: Based on Transcriptomic Analysis

Won

Kang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Mitochondrial targeted therapy is a next-generation therapeutic approach for cancer that is refractory to conventional treatments. Mitochondrial damage caused by the excessive accumulation of reactive oxygen species (ROS) is a principle of mitochondrial targeted therapy. ROS in nonthermal plasma-activated media (NTPAM) are known to mediate anticancer effects in various cancers including head and neck cancer (HNC). However, the signaling mechanism of HNC cell death via NTPAM-induced ROS has not been fully elucidated. This study evaluated the anticancer effects of NTPAM in HNC and investigated the mechanism using transcriptomic analysis. The viability of HNC cells decreased after NTPAM treatment due to enhanced apoptosis. A human fibroblast cell line and three HNC cell lines were profiled by RNA sequencing. In total, 1 610 differentially expressed genes were identified. Pathway analysis showed that activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP) were upstream regulators. Mitochondrial damage was induced by NTPAM, which was associated with enhancements of mitochondrial ROS (mtROS) and ATF4/CHOP regulation. These results suggest that NTPAM induces HNC cell death through the upregulation of ATF4/CHOP activity by damaging mitochondria via excessive mtROS accumulation, similar to mitochondrial targeted therapy.

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Formation of reactive oxygen species by irradiation of cold atmospheric pressure plasma jet to water depends on the irradiation distance

Cited by 19 publications

References 23 publications

Cold Atmospheric Plasma Modification of Amyloid β

Cold Atmospheric Plasma Modification of Amyloid β

A KHz frequency cold atmospheric pressure argon plasma jet for the emission of O(1S) auroral lines in ambient air

Head and Neck Cancer Cell Death due to Mitochondrial Damage Induced by Reactive Oxygen Species from Nonthermal Plasma‐Activated Media: Based on Transcriptomic Analysis

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