Formation of secretion granules in the Golgi apparatus of plasma cells in the rat

Abstract

The three-dimensional structure of the components of the Golgi apparatus was analyzed in plasma cells of rat duodenum. The spheroidal juxtanuclear Golgi apparatus was formed by a continuous ribbonlike structure composed of the following stacked elements. On the cis-face of the Golgi stack, there was a tubular membranous network referred to as the cis-element and/or a slightly dilated saccule perforated with small pores. The two or three subjacent saccules, which showed few pores, were slightly dilated and contained a fluffy granulofilamentous material. They were also perforated in register by cavities or wells containing 80-nm vesicles. The next one or two underlying elements were fenestrated saccules showing flattened portions as well as distended portions containing a homogeneous material denser than that seen in the overlying saccules. The last two or three elements of the stack showed a partially separated or "peeling off" configuration. These last elements consisted of prosecretory granules attached to flattened, empty-looking saccules showing buds at their surface; detached, more-or-less fenestrated, flattened saccules; and shrivelled residual trans-tubular networks. In the trans-region of the stack, in addition to numerous small vesicles, short membranous tubules, detached prosecretory granules, and denser fully formed secretion granules were also seen. These images were interpreted to indicate that secretory material present in the trans-saccules flows toward the dilated portions which become prosecretory granules. The trans-most elements seemingly peel off the stack to yield prosecretory granules and fragmenting trans-tubular networks.

Segregation of secretory material in all elements of the Golgi apparatus in principal epithellal cells of the rat seminal vesicle

“…Characteristically, no corona of small vesicles were ever noted around prosecretory granules or even around the mature secretory granules seen at the apex of the cell where they appeared to be reducing in size. This is in contrast to what has been observed around the forming and condensing granules of mucous cells of Brunner's gland (Rambourg et al, 1987a1, pancreatic acinar cells (Rambourg et al, 19881, and plasma cells (Rambourg et al, 1989).…”

The structure of the Golgi apparatus: a sperm's eye view in principal epithelial cells of the rat epididymis

“…Vesicular tubular clusters have also been noted in various cell types in vivo such as pancreatic acinar cells (Jamieson and Palade 1967), plasma cells (Rambourg et al 1989), and prostatic epithelial cells (L. Hermo and Y. Clermont, unpublished). They have also been noted in various cultured cells where they are generally seen at multiple sites in the peripheral cytoplasm (Saraste and Svensson 1991;Saraste and Kuismanen 1992;Pind et al 1994;Stinchcombe et al 1995;Bannykh et al 1996;Lotti et al 1996;Bannykh and Balch 1997).…”

Three‐dimensional structure of cytidine monophosphatase reactive trans‐Golgi elements in spinal ganglion cells of the rat

“…Tri-dimensional analyses of the trans-compartment in exocrine secretory cells, such as the mucous cells of Brunner's gland, pancreatic acinar cells, and plasma cells, strongly suggested that the formation and liberation of secretory progranules were concomitant with and probably resulted from the progressive fenestration and rupture of the trans elements (Rambourg et al, 1987b(Rambourg et al, , 1988(Rambourg et al, , 1989. In the present study of ganglion cells, it was observed that fenestrated spheres seemingly derive from large fragments of peeling-off TGNs.…”