Formation of Silver Nanoparticles Using Fluorescence Properties of Chitosan Oligomers

Abstract

In this study, silver chloride nanoparticles (AgCl NPs) were prepared using chitosan oligomer (CHI) and chitosan oligomer derivatives (CHI-FITC). The CHI and CHI-FITC were used as markers to confirm the formation of AgCl NPs using their fluorescence properties as well as stabilizers. The fluorescence properties of CHI and CHI-FITC were monitored by a luminescence spectrophotometer, and the morphology of the AgCl NPs was further confirmed by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The fluorescence of CHI and CHI-FITC was quenched by the formation of AgCl NPs, and the Stern–Volmer equation was used to compare the two types of stabilizer. The CHI and CHI-FITC stabilizer were linear and nonlinear, respectively, with respect to the Stern–Volmer equation, and considered to be usable as fluorescence indicators to confirm the formation behavior of AgCl NPs through fluorescence quenching.

Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers

“…FITC-labeled CS-BR was prepared as previously reported. 25 , 26 In brief, a CS-BR solution was added to an ethanol solution of FITC, and the mixture was stirred at room temperature for 12 h in a dark room. The mixture solution was then dialyzed using a 7-kDa dialysis membrane for 8 h against distilled water under light-proof conditions and was lyophilized to obtain FITC-labeled CS-BR.…”