Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

Vockenroth, Inga K.; Rossi, Claire; Shah, Muhammad Raza; Köper, Ingo

doi:10.1116/1.3122019

Cited by 49 publications

(80 citation statements)

References 41 publications

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“…Measurements were performed with the Q-Sense E1 instrument (Q-Sense, Gothenburg, Sweden) as described in [21] . Protein solutions (2 mL, 100μg/mL) were pumped through the sensor chamber by a peristaltic pump at a flow rate of 500μL/min.…”

Section: Methodsmentioning

confidence: 99%

Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides

et al. 2013

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Soft tissue adhesion on titanium represents a challenge for implantable materials. In order to improve adhesion at the cell/material interface we used a new approach based on the molecular recognition of titanium by specific peptides. Silk fibroin protein was chemically grafted with titanium binding peptide (TiBP) to increase adsorption of these chimeric proteins to the metal surface. Quartz Crystal Microbalance was used to quantify the specific adsorption of TiBP-functionalized silk and an increase in protein deposition by more than 35% was demonstrated due to the presence of the binding peptide. A silk protein grafted with TiBP and fibronectin-derived RGD peptide was then prepared. The adherence of fibroblasts on the titanium surface modified with the multifunctional silk coating demonstrated an increase in the number of adhering cells by 60%. The improved adhesion was demonstrated by Scanning Electron Microscopy and immunocytochemical staining of focal contact points. Chick embryo organotypic culture also revealed strong adhesion of endothelial cells expanding on the multifunctional silk-peptide coating. These results demonstrated that silk functionalized with TiBP and RGD represents a promising approach to modify cell-biomaterial interfaces, opening new perspectives for implantable medical devices, especially when reendothelialization is required.

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Section: Methodsmentioning

confidence: 99%

Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides

et al. 2013

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“…The synthesized anchor lipids were composed of three parts, i.e., a hydrophobic moiety that inserted into the bilayer, a short spacer decoupling the membrane from the substrate, and a surface-specific anchor group ( Figure 3). 9,43 The alkyl chains of the hydrophobic part were modified with diacetylene groups, which enables the cross-linking of the proximal leaflet of the membrane to form diffusion barriers upon polymerization. 26 Long fatty acid chains with diacetylene groups were chosen in order to allow for the formation of a light-sensitive crystal with the ability to polymerize at room temperature.…”

Section: ' Introductionmentioning

confidence: 99%

Nanoscale Patterning of Solid-Supported Membranes by Integrated Diffusion Barriers

2011

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Ultraflat nanostructured substrates have been used as a template to create patterned solid-supported bilayer membranes with polymerizable tethered lipids acting as diffusion barriers. Patterns in the size range of 100 nm were successfully produced and characterized. The diffusion barriers were embedded directly into the phospholipid bilayer and could be used to control the fluidity of the membrane as well as to construct isolated membrane corrals. By using nanosphere lithography to structure the templates it was possible to systematically adjust the lipid diffusion coefficients in a range comparable to those observed in cellular membranes. Single colloids applied as mask in the patterning process yielded substrates for creation of isolated fluid membrane patches corralled by diffusion barriers. Numerous potential applications for this new model system can be envisioned, ranging from the study of cellular interactions or of molecular diffusion in confined geometries to biosensor arrays.

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“…The DE content was varied up to a 0.88 molar fraction; more than this limit, the amine induced disorder of the acyl chains of PC, and we were not able to form a bilayer stable enough to carry out measurements on it. The impedance technique was used in our study to characterize the membrane features as this method has been shown to measure the capacitance and resistance of bilayer lipid membranes accurately [26, 27]. The mean values of the determined parameters were obtained based on six independent measurements of the lipid bilayer.…”

Section: Resultsmentioning

confidence: 99%

Impedance Analysis of Complex Formation Equilibria in Phosphatidylcholine Bilayers Containing Decanoic Acid or Decylamine

Naumowicz

Petelska

Figaszewski

2011

Cell Biochem Biophys

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Bilayer lipid membranes composed of phosphatidylcholine and decanoic acid or phosphatidylcholine and decylamine were investigated using electrochemical impedance spectroscopy. Interaction between membrane components causes significant deviations from the additivity rule. Area, capacitance, and stability constant values for the complexes were calculated based on the model assuming 1:1 stoichiometry, and the model was validated by comparison of these values to experimental results. We established that phosphatidylcholine and decylamine form highly stable 1:1 complexes. In the case of decanoic acid-modified phosphatidylcholine membranes, complexes with stoichiometries other than 1:1 should be taken into consideration.

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Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

Cited by 49 publications

References 41 publications

Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides

Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides

Nanoscale Patterning of Solid-Supported Membranes by Integrated Diffusion Barriers

Impedance Analysis of Complex Formation Equilibria in Phosphatidylcholine Bilayers Containing Decanoic Acid or Decylamine

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