Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in Emericellopsos alkalina under Different Cultivation Conditions

Куварина, А. Е.; Sukonnikov, M. A.; Рогожин, Е. А.; Serebryakova, Marina V.; Тимофеева, А. В.; Георгиева, М. Л.; Садыкова, В. С.

doi:10.1134/s0003683823020060

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…All chemicals and reagents used in the study were obtained from commercial vendors, including Merck (Germany) and Sigma Aldrich Sigma Chemical, Co. (USA), and were utilized without further purification. HPLC was performed using an HPLC microcolumn chromatograph (Millichrom A-02, RF) 62 , 63 with a gradient of acetonitrile (5–100%)/0.1 M chloric acid and 0.5 M lithium perchlorate).…”

Section: Methodsmentioning

confidence: 99%

Molecular Modeling, Synthesis, and Antihyperglycemic Activity of the New Benzimidazole Derivatives – Imidazoline Receptor Agonists

Martynov,

Farber,

Bomko

et al. 2024

DDDT

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Introduction The paper presents the results of a study on the first synthesized benzimidazole derivatives obtained from labile nature carboxylic acids. The synthesis conditions of these substances were studied, their structure was proved, and some components were found to have sugar-reducing activity on the model of alloxan diabetes in rats. Methods The study used molecular modeling methods such as docking based on the evolutionary model (igemdock), RP_HPLC method to monitor the synthesis reaction, and 1H NMR and 13C NMR, and other methods of organic chemistry to confirm the structures of synthesized substances. Results & Discussion The docking showed that the ursodeoxycholic acid benzimidazole derivatives have high tropics to all imidazoline receptor carriers (PDB ID: 2XCG, 2bk3, 3p0c, 1QH4). The ursodeoxycholic acid benzimidazole derivative and arginine and histidine benzimidazole derivatives showed the highest sugar-lowering activity in the experiment on alloxan-diabetic rats. For these derivatives, the difference in glucose levels of treated rats was significant against untreated control. Therefore, the new derivatives of benzimidazole and labile natural organic acids can be used to create new classes of imidazoline receptor inhibitors for the treatment of diabetes mellitus and hypertension.

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Section: Methodsmentioning

confidence: 99%

Molecular Modeling, Synthesis, and Antihyperglycemic Activity of the New Benzimidazole Derivatives – Imidazoline Receptor Agonists

Martynov,

Farber,

Bomko

et al. 2024

DDDT

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show abstract

“…To date, studies with Emericellopsis sp. have focused on the production of novel antimicrobial and cytotoxic compounds [63][64][65][66][67][68].…”

Section: Electrophoretic Separation Of Proteins In the Crude Secretom...mentioning

confidence: 99%

Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

Dowd,

Tuohy

2023

Fermentation

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Fungi are increasingly recognised as being able to inhabit extreme environments. The deep sea is considered an extreme environment because of its low temperatures, high hydrostatic and lithostatic pressures, 3.5% salinity, and low oxygen, nutrient and light availability. Fungi inhabiting the deep sea may have evolved to produce proteins that allow them to survive these conditions. Investigation and characterisation of fungal lignocellulolytic enzymes from extreme environments like the deep sea is needed, as they may have unusual adaptations that would be useful in industry. This work, therefore, aimed to profile in detail the lignocellulolytic capabilities of fungi isolated from deep-sea sediments in the Atlantic Ocean, and a comparative lignocellulolytic terrestrial isolate. The isolates were strains of Emericellopsis maritima, Penicillium chrysogenum, P. antarcticum and Talaromyces stollii. Lignocellulolytic enzyme induction was achieved using liquid-state fermentation (LSF) with wheat bran as the main carbon source, while enzyme characteristics were evaluated using biochemical assays and gel-based proteomics. This study revealed that the isolates were halotolerant, produced xylanase over wide pH and temperature ranges, and produced a variety of glycoside hydrolase and feruloyl esterase activities. The T. stollii secretome demonstrated remarkable levels of exo-glycoside hydrolase activity, with xylanase activity optimum between pH 1.5–6.0 and temperatures between 1–60 °C, making this isolate an ideal candidate for biotechnological applications. This study is the first to quantitatively characterise xylanase activities and exo-glycoside hydrolase activities secreted by E. maritima, P. antarcticum and a marine T. stollii strain. This study is also the first to quantitatively characterise xylanase activities by a marine strain of P. chrysogenum during LSF.

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Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in Emericellopsos alkalina under Different Cultivation Conditions

Cited by 2 publications

References 15 publications

Molecular Modeling, Synthesis, and Antihyperglycemic Activity of the New Benzimidazole Derivatives – Imidazoline Receptor Agonists

Molecular Modeling, Synthesis, and Antihyperglycemic Activity of the New Benzimidazole Derivatives – Imidazoline Receptor Agonists

Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

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