Formation of Vascularized Meniscal Tissue by Combining Gene Therapy with Tissue Engineering

Hidaka, Chisa; Ibarra, Clemente; Hannafin, Jo A.; Torzilli, Peter A.; Quitoriano, Mannix; Jen, S. C.; Warren, Russell F.; Crystal, Ronald G.

doi:10.1089/107632702753503090

Cited by 91 publications

(45 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This approach resulted in alignment and migration of meniscal cells toward the defect, which was not observed in untreated controls [2]. In other research related to gene-modified bovine meniscal cells, adenovirusmediated hepatocyte growth factor gene therapy enhanced blood vessel formation in the subcutaneous pouch of athymic nude mice for 8 weeks [9]. The current research selected the hIGF-1 as the target cytokine because it plays a key role in the regulation of chondrocyte proteoglycan metabolism [21,25]: it is an important anabolic modulator of cartilage metabolism whose action is mediated by high-affinity cell surface receptors.…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Zhang

Leng

Wang

et al. 2009

Clin Orthop Relat Res

View full text Add to dashboard Cite

The menisci are intraarticular fibrocartilaginous structures essential to the normal function of the knee that lack the ability to self-repair. Human meniscal fibrochondrocytes may respond to beneficial genes like human insulin growth factor-1 (hIGF-1) and the meniscal cell may be a feasible donor for gene therapy. To explore this possibility, we amplified the hIGF-1 gene sequence in full length and cloned it into a bicistronic plasmid. This gene was then transfected into cultured human meniscal fibrochondrocytes by the liposome FuGene 6. Green fluorescence was expressed in part of the cells 6 hours after transfection and increased gradually, with a peak concentration of the hIGF-1 in the supernatants to 22.68 ng/mL 56 hours after transfection. Phenotypes of some cells changed and the proliferation accelerated after transfection. Flow cytometry analysis demonstrated upregulation of cell numbers in the G2 and S stages after hIGF-1 gene introduction. We conclude the hIGF-1 gene can be transfected into the human meniscal cell efficiently by liposome and it causes accelerated proliferation and differentiation. Within 10 days after transfection, the cytokine appears to be secreted into supernatants with the bioactivity and promotes the proliferation of the NIH 3T3 cell line.

show abstract

Section: Discussionmentioning

confidence: 93%

“…Some researchers have introduced the gene of growth factors into meniscal tissue or cells to enhance the repair [2,9]. In an in vitro repair of meniscal defects, growth factors could diffuse from a collagen sponge or a collagen gel into meniscal tissue surrounding defects and stimulate the formation of proteoglycan.…”

Section: Discussionmentioning

confidence: 99%

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Zhang

Leng

Wang

et al. 2009

Clin Orthop Relat Res

View full text Add to dashboard Cite

show abstract

“…Other GF demonstrated to have a trophic effect on meniscal fibrochondrocytes are: hepatocyte growth factor (HGF); insulin-like growth Meniscus repair and regeneration factor 1 (IGF-1); fibroblast growth factor 2 (FGF-2); platelet-derived growth factor (PDGF); and certain bone morphogenetic proteins (BMPs) (Buma et al, 2004, Pangborn andAthanasiou, 2005). In particular, HGF was demonstrated to enhance vascularisation of engineered meniscal fibrochondrcyte-PGA constructs, but without improving mechanical properties (Hidaka et al, 2002), and IGF-1 is one of the main anabolic factors of articular cartilage (Schmidt et al, 2006). As the outer part of the meniscus is vascularised (Clark and Ogden, 1983) and the inner region is characterised by an ECM partially similar to that of the articular cartilage (Chevrier et al, 2009), the use of these two GF could be a promising combination to be evaluated.…”

Section: Growth Factors and Gene Therapymentioning

confidence: 99%

“…Recent advances in gene therapy techniques have demonstrated the feasibility of gene transfer to musculoskeletal tissues Madry et al, 2003;Madry et al, 2004). GF gene transfer is an attractive option to enhance meniscal repair, and this has been demonstrated both in vivo and in vitro with different approaches (Goto et al, 1999;Hidaka et al, 2002;Martinek et al, 2002;Nakata et al, 2001). Several vectors have been used: retroviral, adenoviral and adenoassociated; each of them presenting peculiar characteristics (Madry et al, 2004;Goto et al, 1999;Hidaka et al, 2002).…”

Section: Growth Factors and Gene Therapymentioning

confidence: 99%

Meniscus repair and regeneration: review on current methods and research potential

Scotti

Hirschmann

Antinolfi

et al. 2013

eCM

130

123

View full text Add to dashboard Cite

Meniscus regeneration is an unsolved clinical challenge. Despite the wide acceptance of the degenerative consequences of meniscectomy, no surgical procedure has succeeded to date in regenerating a functional and long-lasting meniscal fibrocartilage. Research proposed a number of experimental approaches encompassing all the typical strategies of regenerative medicine: cellfree scaffolds, gene therapy, intra-articular delivery of progenitor cells, biological glues for enhanced bonding of reparable tears, partial and total tissue engineered meniscus replacement. None of these approaches has been completely successful and can be considered suitable for all patients, as meniscal tears require specific and patientrelated treatments depending on the size and type of lesion. Recent advances in cell biology, biomaterial science and bioengineering (e.g., bioreactors) have now the potential to drive meniscus regeneration into a series of clinically relevant strategies. In this tutorial paper, the clinical need for meniscus regeneration strategies will be explained, and past and current experimental studies on meniscus regeneration will be reported.

show abstract

“…The cell cultures infected with the TGF-betacarrying viruses contained much higher concentrations of TGF-beta, collagen, and proteoglycans compared with the control group. Similarly, Hidaka et al [49] proposed to improve the conditions for meniscal vascularization via gene therapy, which in theory could improve the prognosis of meniscal healing and repair. By using adenoviruses as vectors for the hepatocyte growth factor gene to induce neovascularization in the bovine meniscus, the authors obtained encouraging results.…”

Section: Growth Factors and Gene Therapymentioning

confidence: 99%

Updates in biological therapies for knee injuries: menisci

Kaleka

Debieux

Astur

et al. 2014

Curr Rev Musculoskelet Med

View full text Add to dashboard Cite

The preservation of meniscal tissue is paramount for long-term joint function, especially in younger patients who are athletically active. Many studies have reported encouraging results following the repair of meniscus tears, including both simple longitudinal tears located in the periphery and complex multiplanar tears that extend into the central third avascular region. However, most types of meniscal lesions are managed with a partial meniscectomy. Options to restore the meniscus range from an allograft transplantation to the use of synthetic and biological technologies. Recent studies have demonstrated good long-term outcomes with meniscal allograft transplantation, although the indications and techniques continue to evolve, and the long-term chondroprotective potential of this approach has yet to be determined. Several synthetic implants, most of which are approved in the European market, have shown some promise for replacing part of or the entire meniscus, including collagen meniscal implants, hydrogels, and polymer scaffolds. Currently, there is no ideal implant generated by means of tissue engineering. However, meniscus tissue engineering is a fast developing field that promises to develop an implant that mimics the histologic and biomechanical properties of a native meniscus.

show abstract

Formation of Vascularized Meniscal Tissue by Combining Gene Therapy with Tissue Engineering

Cited by 91 publications

References 45 publications

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Meniscus repair and regeneration: review on current methods and research potential

Updates in biological therapies for knee injuries: menisci

Contact Info

Product

Resources

About