Formation of virions is strictly required for turnip yellows virus long-distance movement in plants

Hipper, Clémence; Monsion, Baptiste; Bortolamiol-Bécet, Diane; Ziegler-Graff, Véronique; Brault, Véronique

doi:10.1099/vir.0.058867-0

Cited by 29 publications

(26 citation statements)

References 40 publications

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“…The finding that P17 associates with the PLRV virion protein interaction network is highly consistent with evidence that shows poleroviruses traffic systemically as virions [47] and that, in N. benthamiana, P17 aids in the efficient, systemic movement of PLRV [48]. A total of 1107 plant proteins were classified as category I proteins.…”

Section: Category I Protein Interactionssupporting

confidence: 84%

Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection

et al. 2015

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Potato leafroll virus (PLRV) produces a readthrough protein (RTP) via translational readthrough of the coat protein amber stop codon. The RTP functions as a structural component of the virion and as a nonincorporated protein in concert with numerous insect and plant proteins to regulate virus movement/transmission and tissue tropism. Affinity purification coupled to quantitative MS was used to generate protein interaction networks for a PLRV mutant that is unable to produce the read through domain (RTD) and compared to the known wild-type PLRV protein interaction network. By quantifying differences in the protein interaction networks, we identified four distinct classes of PLRV-plant interactions: those plant and nonstructural viral proteins interacting with assembled coat protein (category I); plant proteins in complex with both coat protein and RTD (category II); plant proteins in complex with the RTD (category III); and plant proteins that had higher affinity for virions lacking the RTD (category IV). Proteins identified as interacting with the RTD are potential candidates for regulating viral processes that are mediated by the RTP such as phloem retention and systemic movement and can potentially be useful targets for the development of strategies to prevent infection and/or viral transmission of Luteoviridae species that infect important crop species.

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Section: Category I Protein Interactionssupporting

confidence: 84%

Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection

et al. 2015

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“…) was gained by analysing pumpkin phloem sap (Xoconostle‐Cazares et al ., ; Yoo et al ., ). It is well established that viral RNAs in the phloem are always found in ribonucleoprotein complexes (RNPs) with virally produced proteins (Hipper et al ., ). In addition, host factors can assist virus phloem transport.…”

Section: Phloem As a Conduit For Macromoleculesmentioning

confidence: 97%

Long distance RNA movement

Kehr¹,

2018

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Contents Summary29I.Introduction29II.Phloem as a conduit for macromolecules30III.Classes of phloem transported RNAs and their function32IV.Mode of RNA transport35V.Conclusions37Acknowledgements37References37 Summary In higher plants, small noncoding RNAs and large messenger RNA (mRNA) molecules are transported between cells and over long distances via the phloem. These large macromolecules are thought to get access to the sugar‐conducting phloem vessels via specialized plasmodesmata (PD). Analyses of the phloem exudate suggest that all classes of RNA molecules, including silencing‐induced RNAs (siRNAs), micro RNAs (miRNAs), transfer RNAs (tRNAs), ribosomal RNA (rRNAs) and mRNAs, are transported via the vasculature to distant tissues. Although the functions of mobile siRNAs and miRNAs as signalling molecules are well established, we lack a profound understanding of mobile mRNA function(s) in recipient cells and tissues, and how they are selected for transport. A surprisingly high number of up to thousands of mRNAs were described in diverse plant species such as cucumber, pumpkin, Arabidopsis and grapevine to move long distances over graft junctions to distinct body parts. In this review, we present an overview of the classes of mobile RNAs, the potential mechanisms facilitating RNA long‐distance transport, and the roles of mobile RNAs in regulating transcription and translation. Furthermore, we address potential function(s) of mobile protein‐encoding mRNAs with respect to their characteristics and evolutionary constraints.

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“…In plants, polerovirus infection is limited to phloem tissues: cell-to-cell movement occurs between vascular parenchyma cells, companion cells and the enucleated sieve elements whereas long-distance movement follows the sieve elements. Because polerovirus particles were detected in plasmodesmata connecting phloem cells [3]–[5], the virus is thought to move in the form of virions and recent data confirmed that particles of Turnip yellows virus (TuYV, Polerovirus genus) are essential for long-distance movement [6]. Polerovirus have isometric particles of about 25 nm of diameter containing two structural proteins: the major coat protein (CP) encoded by ORF3 and a minor component, referred to as the readthrough (RT) protein, which is synthesized after ribosomes bypass the ORF3 stop codon during translation.…”

Section: Introductionmentioning

confidence: 96%

Both Structural and Non-Structural Forms of the Readthrough Protein of Cucurbit aphid-borne yellows virus Are Essential for Efficient Systemic Infection of Plants

et al. 2014

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Cucurbit aphid-borne yellows virus (CABYV) is a polerovirus (Luteoviridae family) with a capsid composed of the major coat protein and a minor component referred to as the readthrough protein (RT). Two forms of the RT were reported: a full-length protein of 74 kDa detected in infected plants and a truncated form of 55 kDa (RT*) incorporated into virions. Both forms were detected in CABYV-infected plants. To clarify the specific roles of each protein in the viral cycle, we generated by deletion a polerovirus mutant able to synthesize only the RT* which is incorporated into the particle. This mutant was unable to move systemically from inoculated leaves inferring that the C-terminal half of the RT is required for efficient long-distance transport of CABYV. Among a collection of CABYV mutants bearing point mutations in the central domain of the RT, we obtained a mutant impaired in the correct processing of the RT which does not produce the RT*. This mutant accumulated very poorly in upper non-inoculated leaves, suggesting that the RT* has a functional role in long-distance movement of CABYV. Taken together, these results infer that both RT proteins are required for an efficient CABYV movement.

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Formation of virions is strictly required for turnip yellows virus long-distance movement in plants

Cited by 29 publications

References 40 publications

Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection

Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection

Long distance RNA movement

Both Structural and Non-Structural Forms of the Readthrough Protein of Cucurbit aphid-borne yellows virus Are Essential for Efficient Systemic Infection of Plants

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