— HIV education and tests are the main entrance for prevention, care and treatment support. Video as an educational tool, convey clear, consistent, and unbiased information while easy to use, effective as face-to-face interactions, yet, online-video has never been used in previous studies regardless of its easy-dissemination. Therefore, health cadres hold important tasks in educating people about the importance of HIV. To determine onlinevideo effectiveness on knowledge increment and cadres’ attitude toward HIV testing importance in Public Health Centers. This study used a cross-sectional method with one group pretest and posttest models. The research sample was 50 HIV cadres using a total sampling technique. Data were analyzed using McNemar test to determine the comparison between pretest and posttest of knowledge and attitude variables. Research findings that before and after the intervention was carried out, there were differences in the results on the knowledge and attitude variables. Based on the McNemar test for the variables of knowledge and attitudes obtained significant results (p = 0.00). This research concludes that o nline-video is effective in increasing knowledge and attitudes of HIV cadres toward the importance of HIV testing in Public Health Centers. In addition, it is necessary to have a combination of other interventions (FGD, flip sheets, counseling, leaflets) using a cohort or RCT method with a larger sample