Formolysis of a potent antitumor (1 → 6)-β-d-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products

Kawagishi, Hirokazu; Kanao, Teturo; Inagaki, Ryuichi; Mizuno, Takashi; Shimura, Keishiro; Itô, Haruo; Hagiwara, Toshihiko; Nakamura, Tomoyuki

doi:10.1016/0144-8617(90)90089-b

Cited by 106 publications

(54 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, β-glucans containing mainly 1→6 linkages exhibit less activity, possibly due to their inherent flexibility of having too many possible conformations (Zhang et al, 2007;Ferreira et al, 2010). However, antitumor polysaccharides may have other chemical structures, such as hetero-β-glucans (Mizuno et al, 1995b), heteroglycan (Gao et al, 1996), β-glucan-protein (Kawagishi et al, 1990), α-manno-β-glucan (Mizuno et al, 1995b), α-glucan-protein (Mizuno et al, 1995b) and heteroglycan-protein complexes Mizuno et al, 1996).…”

Section: G Lucidum Which Contained β-(1→4)- β-(1→6)-and β-(1→3)-d-mentioning

confidence: 99%

Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

et al. 2015

View full text Add to dashboard Cite

This review aims to contribute to the growing knowledge on bioactive (antioxidant, antitumor and antimicrobial) properties of polysaccharides, glycoproteins and polysaccharidic extracts obtained from Ganoderma species. The chemical features were analyzed and related to correspondent bioactivities. ____________________________________________________________________ 2 Research highlightsChemical features of Ganoderma bioactive polysaccharides were discussed.Methods for extraction, isolation and identification were evaluated.Bioactivity of polysaccharidic extracts and purified compounds were discussed.Integration of data allowed deduction of structure-activity relationships.

show abstract

Section: G Lucidum Which Contained β-(1→4)- β-(1→6)-and β-(1→3)-d-mentioning

confidence: 99%

Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Heinemann) (25), also denoted A. subrufescens Peck (10), is a Basidiomycete that has been reported because of its antitumoral substances (2,9,16). Reports …”

Section: Agaricus Brasiliensis Wasser Et Al (A Blazei Murrill Ssmentioning

confidence: 99%

Antineoplasic activity of Agaricus brasiliensis basidiocarps on different maturation phases

Mourão¹,

Linde²,

Messa³

et al. 2009

Braz. J. Microbiol.

View full text Add to dashboard Cite

The fungus Agaricus brasiliensis is a Basidiomycete studied because of its immunomodulation and/or antitumor substances. The objective of this study was to verify the Agaricus brasiliensis antineoplasic activity in vivo on different basidiocarp maturation phases on Sarcoma 180 cells implanted in mice.Sarcoma cells were implanted in mice and after seven days mice were divided in three groups. The first group was treated with saline solution, the second group was treated with closed basidiocarp extract solution and the third group was treated with opened basidiocarp extract solution. After 30 days of being daily orally treated with these three solutions all animals suffered euthanasia, and the splenic index, tumor mass and volume were determined. No significant differences of the tumor growth inhibition in function of the different basidiocarp maturation phases for the Agaricus brasiliensis strain were observed. The in vivo basidiocarp antineoplasic average activity was 89.22%.

show abstract

mentioning

confidence: 99%

“…The hot water extract of A. blazei has potent antitumor activity in sarcoma 180-bearing mice, [3][4][5][6] and the antitumor activity was postulated to reside in the β-(1-6) glucan fraction. [3][4][5][6] Previously we examined the antitumor activities of various substances isolated from the lipid fraction of A. blazei and identified ergosterol as an active substance. 7) We found that ergosterol inhibited tumor-induced neovascularization.…”

mentioning

confidence: 99%

Isolation of an anti‐angiogenic substance from Agaricus blazei Murill: Its antitumor and antimetastatic actions

et al. 2004

View full text Add to dashboard Cite

We previously found that ergosterol isolated from Agaricus blazei inhibited tumor growth through the inhibition of tumor-induced neovascularization. In the present study, we isolated further anti-angiogenic substances (A-1 and A-2) from this fungus using an assay system of angiogenesis induced by Matrigel supplemented with vascular endothelial growth factor, and A-1 was identified as sodium pyroglutamate. Next, we examined the antitumor and antimetastatic actions of A-1 using Lewis lung carcinoma ( he Basiodiomycete fungus Agaricus blazei Murill (Japanese name: Himematsutake or Agarikusutake) has been traditionally used as a health food in Brazil for the prevention of cancer, diabetes, hyperlipidemia, arteriosclerosis and chronic hepatitis. It has been reported that 200,000 to 400,000 kg of the dried body of A. blazei is produced every year in Japan.1, 2) A. blazei is used by 300,000 to 500,000 persons for the prevention of cancer and/or as an adjuvant with cancer chemotherapy drugs after the removal of a malignant tumor.1, 2) The water extract of A. blazei is generally administered at 3 to 5 g three times daily. The hot water extract of A. blazei has potent antitumor activity in sarcoma 180-bearing mice, [3][4][5][6] and the antitumor activity was postulated to reside in the β-(1-6) glucan fraction. 3-6)Previously we examined the antitumor activities of various substances isolated from the lipid fraction of A. blazei and identified ergosterol as an active substance. 7) We found that ergosterol inhibited tumor-induced neovascularization.7) Furthermore, we found that the acetone-soluble fractions of ethanol extracts inhibited tumor growth and lung metastasis in Lewis lung carcinoma (LLC)-bearing mice, and inhibited Matrigel-induced angiogenesis (unpublished data). In a preliminary experiment, we found a large amount of D-mannitol in the acetonesoluble fraction of the ethanol extracts. However, D-mannitol had no effect on Matrigel-induced angiogenesis in vivo (data not shown). Therefore, we prepared the methanol-soluble fraction of the chloroform-methanol (1:1, v/v) extracts in order to remove D-mannitol. We then attempted to isolate antiangiogenic substances from the methanol-soluble fraction, which showed antitumor and antiangiogenic activities. We isolated two active substances (A-1 and A-2) from A. blazei with the aid of a Matrigel-induced angiogenesis assay system. This report describes the determination of the structure of A-1 and the effects of A-1 on tumor growth and lung metastasis in LLC-bearing mice. In addition, to clarify the mechanisms of the antitumor and antimetastatic activities of A-1, we examined the effects of A-1 on splenic immune function, tumor apoptosis and tumorinduced neovascularization. Materials and MethodsGeneral experimental procedures. The 1 H-NMR (499.83 MHz) and 13 C-NMR (125.68 Hz) spectra were recorded in D 2 O with a Varian Unity Inova 500 spectrometer (Tosoh, Tokyo). Mass spectra were measured with a Hitachi M-4000 H spectrometer (Tokyo). Thin layer chromatography (TLC), prepar...

show abstract

Formolysis of a potent antitumor (1 → 6)-β-d-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products

Cited by 106 publications

References 11 publications

Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

Antineoplasic activity of Agaricus brasiliensis basidiocarps on different maturation phases

Isolation of an anti‐angiogenic substance from Agaricus blazei Murill: Its antitumor and antimetastatic actions

Contact Info

Product

Resources

About