Formulation and Antioxidant Investigation of Creams Containing Robiniae Pseudacaciae Flos L. Ethanolic Extract

Jurca, Tünde

doi:10.31925/farmacia.2021.4.9

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…Hallmann [ 31 ] reported a tenfold lower TAC of about 3.91 mg/g d.w. for the 80% ( v / v ) methanolic extract obtained via maceration with stirring. Unlike this extract, the lyophilized 70% ( v / v ) ethanolic extract had almost tenfold higher TAC of 311.93 ± 0.01 mg GAE/g d.w. according to Jurca et al [ 32 ]. These data indicate that the TAC depends on the extraction technique and solvent used.…”

Section: Resultsmentioning

confidence: 99%

“…The comparison of obtained data is very hard because of the different units used. According to the DPPH assay, the lyophilized 70% ( v / v ) ethanolic extract of black locust flower had an antioxidant capacity of 3.58 ± 0.11% [ 32 ]. Bratu et al [ 33 ] determined the antioxidant activity of ethanolic extract to be 0.141 ± 0.02 μM Trolox equivalent/mL using DPPH assay.…”

Section: Resultsmentioning

confidence: 99%

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Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Boškov,

Savić,

Grozdanić Stanisavljević

et al. 2024

Polymers

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Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Boškov,

Savić,

Grozdanić Stanisavljević

et al. 2024

Polymers

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“…The DPPH assay was prepared according to Brand-Williams recommendations, with slight modifications [22,23]. The stock solution was used at a 0.1 mM final concentration: 2 mg of DPPH reagent were suspended in 50 mL of ethanol 96%.…”

Section: Dpph Methodsmentioning

confidence: 99%

Physico-Chemical Characterization of the Antioxidant Mixture Resveratrol-Ferulic Acid for Applications in Dermato-Cosmetic Products

Turcov¹

2022

FARMACIA

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The aim of the paper was to identify the best combination of resveratrol -ferulic acid with the purpose of increasing the antioxidant activity and maintaining the qualities of resveratrol in the management of skin disorders. Aqueous solutions of resveratrol (1%, 3% and 5% concentrations) and hydro alcoholic solution (30%) of ferulic acid were used. The following resveratrol -ferulic acid mixtures (v/v) were considered: 1:1; 1:2; 1:3 and 1:4. The antioxidant activity of the mixtures was determined and compared with that of resveratrol, and also the physico-chemical were performed by thermogravimetric analyses and UV-VIS spectrophotometry. The results underline that the mixtures of 1% and 3% resveratrol with 0.5% ferulic acid in a ratio of 1:1 and 1:2 ensure the improvement of the antioxidant activity of resveratrol. RezumatScopul lucrării a fost de a identifica cea mai bună combinație resveratrolacid ferulic cu scopul de a potența activitatea antioxidantă, menținând calitățile resveratrolului, în gestionarea unor patologii cutanate. S-au folosit soluții apoase de resveratrol (având concentrația 1%, 3% și 5%) și o soluție hidroalcoolică (30%) de acid ferulic. Au fost luate în considerare următoarele amestecuri de resveratrol -acid ferulic (v/v): 1:1; 1:2; 1:3 și 1:4. S-a determinat activitatea antioxidantă a acestor amestecuri și a fost comparată cu cea a resveratrolului. Amestecurile au fost, de asemenea, caracterizate fizico-chimic prin analize termogravimetrice și spectrofotometrice UV-VIS. Rezultatele subliniază că amestecurile de resveratrol 1% și 3% cu acid ferulic 0,5%, în raport de 1:1 și 1:2 asigură îmbunătățirea activității antioxidante a resveratrolului.

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“…Robinia pseudoacacia has strong adaptability to the environment and is widely distributed around the world. Its flower can be used as herbal medicine due to its extensive pharmacological effects, such as antioxidant, hypoglycemic, antibacterial, hemostasis, antiobesity, and so forth [6][7][8][9]. Among them, antioxidant activity has attracted much attention, which mainly depends on its rich flavonoids and phenols [10].…”

Section: Introductionmentioning

confidence: 99%

Elution–extrusion counter‐current chromatographic separation and theoretical mechanism of antioxidant from Robinia pseudoacacia flower

Liu

Zhang

Tang

et al. 2023

J of Separation Science

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Robinia pseudoacacia flowers have attracted much attention because of numerous bioactivities. In this study, its extract showed the potential scavenging ability for 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) and 1,1-diphenyl-2-picrylhydrazyl free radicals. Under the guidance of antioxidant activity, the antioxidant extract was enriched by liquid-liquid extraction. The partition coefficients of the two main components in antioxidant extracts differed greatly, so in this study, elution-extrusion counter-current chromatography with the solvent system of n-hexane-ethyl acetate-methanol-water (2.5:5:2.5:5, v/v) was used to enhance the separation efficiency, and the two main components were successfully obtained. Among them, kaempferol showed strong antioxidant activity, which can be responsible for the activity of the extract. In order to deeply understand the antioxidant mechanism of kaempferol, the thermodynamics, frontier molecular orbital, and kinetics of scavenging free radicals were investigated by density functional theory. The results showed that 4′-OH in kaempferol was the most active group, which can scavenge free radicals by hydrogen atom transfer in non-polar solvents and activate 3-OH to generate double hydrogen atom transfer in the gas phase. But in polar solvents, it was more inclined to clear radicals through single electron transfer and proton transfer. The kinetic result showed that kaempferol needed 9.17 kcal/mol of activation energy to scavenge free radicals.

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Formulation and Antioxidant Investigation of Creams Containing Robiniae Pseudacaciae Flos L. Ethanolic Extract

Cited by 3 publications

References 14 publications

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Physico-Chemical Characterization of the Antioxidant Mixture Resveratrol-Ferulic Acid for Applications in Dermato-Cosmetic Products

Elution–extrusion counter‐current chromatographic separation and theoretical mechanism of antioxidant from Robinia pseudoacacia flower

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