Formulation and Evaluation of Antibacterial Topical Gel of Doxycycline Hyclate, Neem Oil and Tea Tree Oil

Abstract

Purpose: To formulate novel topical antibacterial gel containing Doxycycline Hyclate, Tea tree oil and Neem oil to evaluate their antibacterial activity. Methods: Formulation excipients were selected using Drug-excipients compatibility study. Prepared gel was subjected to various evaluation parameters like pH, viscosity, spreadability, homogeneity, drug content uniformity, in-vitro drug diffusion and antibacterial activity. Results: Prepared topical gel of doxycycline Hyclate was shown pH range 5.3±0.010 to 6.7±0.015, viscosity range 386±28.70 to 680±50.40 cp, spreadability range 18.14±0.98 to 28.13±2.18 g.cm/s and homogeneous. Prepared gel was shown uniformity in drug content which ranges from 94.41±0.61 to 97.44±0.69%, zone of inhibition ranges 13.66±1.69 to 32.00±1.63 mm for Staphylococcus aureus, 04.00±1.63 to 12.33±0.94 mm for Pseudomonas aeruginosa and 11.66±0.94 to 33.33±1.24 mm for Propionibacterium acne. Prepared gel formulations were shown the negligible amount of drug diffusion, not more than 3.22±0.04% was shown the non-fickian type of diffusion through the egg membrane. Conclusion: Formulation F8 was shown better antibacterial activity as compared to its other formulations. As the concentration of neem oil and tea tree oil was increased from 0.5 to 3% and 0.5 to 2% respectively in the gel, antibacterial activity was synergistically improved against Staphylococcus aureous, Pseudomonas aeruginosa and Propionibacterium acne. The prepared gel formulation was found useful for topical application due to its negligible diffusion, good spreadability, neutral pH, low viscosity and no irritation on human skin.

Calcitermin-Loaded Smart Gels Activity against Candida albicans: A Preliminary In Vitro Study

“…Remarkably, an increased antifungal activity by gel inclusion has been shown for several potential antifungal compounds other than Cal, confirming our present findings [57][58][59][60][61][62][63][64]. For instance metal ions [58], and conventional fungicides such as miconazole and voriconazole, showed an increased action by gel inclusion also in vivo, in animal models [62,64].…”

“…For instance metal ions [58], and conventional fungicides such as miconazole and voriconazole, showed an increased action by gel inclusion also in vivo, in animal models [62,64]. Several compounds of natural origin, such as tea tree or other oils, were reported to have a better antifungal activity when included in gel preparation [60]. In addition, gel formulations specifically addressed to treat vaginal dysbiosis (Respecta ® Balance Gel, RBG, marketed as an adjunct to probiotic treatment), showed a high inhibitory action against diverse Candida strains, including C. albicans and C. glabrata, as well as against Gram-negative and grampositive vaginal bacteria [59].…”

Effect of Hydroxypropyl Methylcellulose (Hpmc) as Gelling Agent on Physical Characteristics and Antibacterial Potential of Tamoenju Leaves Extract Gel (Hibiscus Surattensis L) Against Staphylococcus Aureus

“…Table 2 displays the inhibition zone measurement results. Result of this study compared with similar study from Borse [19] using HPMC as a gel base, neem oil and tea tree oil as active agent for acne; there are differences in the viscosity values and inhibition zone for S. aureus bacteria. This difference is due to the higher concentration of HPMC and different active substances to produce different zones of inhibition, while the pH has same value according to the face pH range.…”

Fabrication of Topical Pluronic Lecithin Organogel Containing Immunomodulatory Drug for Site-Specific Drug Delivery

“…19 Further, Rheological behaviour of formulation at speeds of 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 rpm were measured. 20…”