Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Badekova, K. Zh.; Атажанова, Г. А.; Kačergius, Tomas; Ахметова, С. Б.; Smagulov, Marlen Kemelbekovich

doi:10.1016/j.jtumed.2021.05.009

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…Since the effect of the extract on planktonic growth of the dental plaque isolates were found to be only limited in this concentration range, we focussed on the anti-biofilm potential instead. Previous studies have already demonstrated the anti-biofilm potential of O. vulgare extracts against oral bacteria in standard in vitro setups ( Khan et al, 2017 ; de Oliveira Carvalho et al, 2020 ; Badekova et al, 2021 ). For example, Vaca et al (2021) showed that biofilms formed by a mixed culture of Str.…”

Section: Discussionmentioning

confidence: 99%

Origanum vulgare ethanolic extracts as a promising source of compounds with antimicrobial, anti-biofilm, and anti-virulence activity against dental plaque bacteria

Idir

Ginneken²,

Coppola³

et al. 2022

Front. Microbiol.

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Dental caries and periodontal diseases remain a challenge for oral health, especially given the lack of effective and safe treatment options that are currently available. Against the backdrop of an ongoing antimicrobial resistance crisis, a renewed interest in traditional medicinal plants as a potential source of new bioactive compounds has surfaced. In this context, we systematically screened the antimicrobial and anti-biofilm activities of both ethanolic and aqueous extracts of nine Algerian medicinal plants (Artemisia herba alba, Centaurium erythraea, Juglans regia, Laurus nobilis, Matricaria recutita, Mentha pulegium, Mentha piperita, Origanum vulgare and Taraxacum officinale). To evaluate the activity spectrum of the extracts, the screening was carried out against an extensive collection of Streptococcus, Enterococcus and Lacticaseibacillus isolates recovered from dental plaques of Algerian patients. Broad-spectrum antimicrobial and anti-biofilm properties were observed, especially among ethanolic extracts, which marks them as a promising source for bioactive compounds to control oral biofilms. The ethanolic extract of O. vulgare, which showed the most promising effects in the initial screening, was further characterized. We first verified the biocompatibility of this extract using human oral keratinocytes and selected a range of non-cytotoxic concentrations (0.195–0.781 mg/ml) to further validate its anti-biofilm and anti-virulence potential. At these concentrations, the extract not only prevented biofilm formation (10.04 ± 0.75–87.91 ± 9.08% of reduction) of most dental plaque isolates on a polystyrene surface, but also significantly reduced their adherence to hydroxyapatite (34.58 ± 9.09–62.77 ± 0.95%). Moreover, the extract showed curative potential against mature biofilms grown under conditions mimicking the oral niche. In addition to its anti-biofilm properties, we observed an inhibition of glucosyltransferase activity, a reduction in acidogenesis and a downregulation in the expression of multiple virulence-associated genes for extract-treated samples. Since anti-virulence properties are more robust to the development of resistance, they provide an attractive complementation to the antimicrobial activities of the extract. Thymol was identified as an important active compound of the extract using GC–MS analysis, but synergy with other compounds was also detected, suggesting a potential advantage of using the whole extract over purified thymol. Further research into the bioactive compounds of the O. vulgare ethanolic extract could yield novel products to fight dental caries.

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Section: Discussionmentioning

confidence: 99%

Origanum vulgare ethanolic extracts as a promising source of compounds with antimicrobial, anti-biofilm, and anti-virulence activity against dental plaque bacteria

Idir

Ginneken²,

Coppola³

et al. 2022

Front. Microbiol.

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“…[ ] Lithuania: 1.9 % (1) [Badekova, 2021] Morocco: 1.9% (1) [Moukhfi, 2022] Moldavia: 1.9% (1) Whole plant: 1.9% (1) [Drinic, 2020] Thailand: 1.9% (1) Total: 100% (53) Total: 100% (53) Total: 100% (52)…”

Section: Rosmarinus Officinalismentioning

confidence: 99%

“…[ Stanojevic, 2016] 220 °C: 22.3% (2) [Özer et al, 2018; 3 hours: 56.0% (28) [Atazhanova, 2022;Badawy, 2014;Badekova, 2021;Béjaoui, 2013;Brito, 2021;Giuliani, 2013;Khan, 2018;Khan, 2019;La Pergola, 2017;Licata, 2015;Mancini, 2014;Moghrovyan, 2019;Morshedloo, 2017;Moukhfi, 2022;Napoli, 2020;Stanojevic, 2016;Tanasescu, 2019;Teixeira, 2013;Tsoumani, 2022;Tuttolomondo, 2016;Zinno, 2023] 90 °C: 11.1% (1) [Sharififard, 2018] 1/1: 7.1%…”

Section: Chemical Constituents Of Essential Oilsmentioning

confidence: 99%

Extraction of essential oils by hydrodistillation of four aromatic species: Conditioning, extraction conditions, yield and chemical composition

Olascuaga-Castillo,

Castillo-Medina,

Villacorta-Zavaleta

et al. 2024

Sci. agropecu.

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Essential oils are one of the most important products in the agricultural and food industry, as they are obtained from aromatic plants using various extraction techniques, such as steam distillation or hydrodistillation. However, these methods present challenges, such as low efficiency, limited selectivity, extensive use of solvents and long extraction times. Moreover, the quality of the oils obtained can be affected by hydrolysis or oxidation due to the duration and amount of water used in the process. This review updates the information on Clevenger-type hydrodistillation extraction of four high-value commercial plant species: Matricaria chamomilla, Rosmarinus officinalis, Origanum vulgare, and Eucalyptus spp. The aim is to evaluate the results of original articles, considering the origin and characteristics of the plant material, extraction conditions, yield and metabolites. The search covered a 10-year period (2013-2023) in Scopus, Web of Science, PubMed, and Google Scholar, using keywords such as species names and terms “essential oils” and “hydrodistillation”, with Boolean connectors such as OR, AND or NOT. The results show that quality and yield are influenced by factors such as geographical regions of origin, plant part used, drying techniques or extraction conditions (plant material/water ratio, extraction temperature, extraction time, etc.). In future research, the conditions associated with the plant material and the extraction process need to be optimized in obtaining high quality essential oils.

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“…The gel components were selected based on their known functional roles in topical formulations, including viscosity modification, pH adjustment, flavor masking and preservative effects. Their quantities were optimized to achieve the desired consistency and properties in the final gel formulation [16], [17, ][18].…”

Section: Formulation Of Antibacterial Oral Gelmentioning

confidence: 99%

Natural antibacterial gel to fight tooth decay: An in-silico modeling approach

Appalaraju Dangeti,

V Venkata Trinadha Anand Kumar Reddy Jeeri,

Prakash Nathaniel Kumar Sarella

et al. 2023

World J. Bio. Pharm. Health Sci.

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This study developed an antibacterial oral gel to inhibit Streptococcus mutans, a bacterium that causes tooth decay. The gel was formulated using methanol extract of Piper betel leaves, hydroxypropyl methylcellulose and glycerin at different concentrations. The formulated gel was evaluated for pH, viscosity and antibacterial activity against S. mutans using standard methods. The methanol extract showed potent antibacterial activity, supporting its use in the gel formulation. In silico molecular docking studies targeted key S. mutans protein residues involved in substrate binding. Eleven bioactive compounds identified in the Piper betel extract via GC-MS analysis were docked against protein 1EUH, an NADP-dependent aldehyde dehydrogenase from S. mutans. All 11 compounds exhibited favorable docking scores, suggesting strong binding affinity for the target protein. The docking results correlated with the antibacterial activity of the formulated gel, which inhibited S. mutans growth more effectively than sodium fluoride, a standard dental product.

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Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Cited by 9 publications

References 17 publications

Origanum vulgare ethanolic extracts as a promising source of compounds with antimicrobial, anti-biofilm, and anti-virulence activity against dental plaque bacteria

Origanum vulgare ethanolic extracts as a promising source of compounds with antimicrobial, anti-biofilm, and anti-virulence activity against dental plaque bacteria

Extraction of essential oils by hydrodistillation of four aromatic species: Conditioning, extraction conditions, yield and chemical composition

Natural antibacterial gel to fight tooth decay: An in-silico modeling approach

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