Formulation of chitosan coated nanoliposomes for the oral delivery of colistin sulfate: in vitro characterization, 99mTc-radiolabeling and in vivo biodistribution studies

Aboumanei, Mohamed H.; Mahmoud, Ashgan F.; Motaleb, M. A.

doi:10.1080/03639045.2021.1908334

Cited by 19 publications

(8 citation statements)

References 64 publications

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“…As observed in Fig. S1 ( supplementary data ), the particle size was increased after stabilization with biosurfactant, which is in coherence with the previous reports (Aboumanei et al, 2021 ; Gorjian et al, 2021 ). After the encapsulation of bioactive agent (nisin) in RS-NCS, particle size further increased.…”

Section: Resultssupporting

confidence: 92%

Active Composite Packaging Reinforced with Nisin-Loaded Nano-Vesicles for Extended Shelf Life of Chicken Breast Filets and Cheese Slices

Niaz

Shabbir

Nооr

et al. 2022

Food Bioprocess Technol

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To meet the demands for more effective and ecofriendly food packaging strategies, the potential of nisin-loaded rhamnolipid functionalized nanofillers (rhamnosomes) has been explored after embedding in hydroxypropyl-methylcellulose (HPMC) and κ-carrageenan (κ-CR)-based packaging films. It was observed that intrinsically active rhamnosomes based nanofillers greatly improved the mechanical and optical properties of nano-active packaging (NAP) films. Incorporation of rhamnosomes resulted in higher tensile strength (5.16 ± 0.06 MPa), Young’s modulus (2777 ± 0.77 MPa), and elongation (2.58 ± 0.03%) for NAP than active packaging containing free nisin (2.96 ± 0.03 MPa, 1107 ± 0.67 MPa, 1.48 ± 0.06%, respectively). NAP demonstrated a homogenous distribution of nanofillers in the biopolymer matrix as elucidated by scanning electron microscopy (SEM). Thermogravimetric analysis (TGA) confirmed that NAP prepared with nisin-loaded rhamnosomes was thermally stable even above 200 °C. Differential scanning calorimetry (DSC) analyses revealed that addition of nisin in nanofillers resulted in a slight increase in Tg (108.40 °C), indicating thermal stability of NAP. Fourier transform infrared spectroscopy (FTIR) revealed slight shift in all characteristic bands of nano-active packaging, which indicated the embedding of rhamnosomes inside the polymer network without any chemical interaction. Finally, when tested on chicken breast filets and cheese slices under refrigerated storage conditions, NAP demonstrated broad-spectrum antimicrobial activity (up to 4.5 log unit reduction) and inhibited the growth of Listeria monocytogenes , Staphylococcus aureus , Pseudomonas aeruginosa , and Escherichia coli . These results suggest that HPMC and κ-CR-based NAP containing functionalized nanofillers can serve as an innovative packaging material for the food industry to improve the safety, quality, and shelf-life of dairy and meat products. Supplementary Information The online version contains supplementary material available at 10.1007/s11947-022-02815-2.

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Section: Resultssupporting

confidence: 92%

Active Composite Packaging Reinforced with Nisin-Loaded Nano-Vesicles for Extended Shelf Life of Chicken Breast Filets and Cheese Slices

Niaz

Shabbir

Nооr

et al. 2022

Food Bioprocess Technol

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“…Aboumanei et al executed in vivo biodistribution studies of thigh muscle Escherichia coli infection-bearing mice with chitosan-coated liposomes encapsulating colistin and showed that upon oral administration, the bioavailability of colistin in the blood was improved more than 5-fold for chitosan-coated liposomes compared to a colistin solution. In addition, the localization of colistin at the infection site was improved by 125-fold for their formulation 1 h postadministration [48]. Thus, the coating of AMP-loaded liposomes with chitosan increases the effectivity and improves the bioavailability of AMP upon oral administration.…”

Section: Liposomesmentioning

confidence: 99%

Current Advances in Lipid and Polymeric Antimicrobial Peptide Delivery Systems and Coatings for the Prevention and Treatment of Bacterial Infections

et al. 2021

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Bacterial infections constitute a threat to public health as antibiotics are becoming less effective due to the emergence of antimicrobial resistant strains and biofilm and persister formation. Antimicrobial peptides (AMPs) are considered excellent alternatives to antibiotics; however, they suffer from limitations related to their peptidic nature and possible toxicity. The present review critically evaluates the chemical characteristics and antibacterial effects of lipid and polymeric AMP delivery systems and coatings that offer the promise of enhancing the efficacy of AMPs, reducing their limitations and prolonging their half-life. Unfortunately, the antibacterial activities of these systems and coatings have mainly been evaluated in vitro against planktonic bacteria in less biologically relevant conditions, with only some studies focusing on the antibiofilm activities of the formulated AMPs and on the antibacterial effects in animal models. Further improvements of lipid and polymeric AMP delivery systems and coatings may involve the functionalization of these systems to better target the infections and an analysis of the antibacterial activities in biologically relevant environments. Based on the available data we proposed which polymeric AMP delivery system or coatings could be profitable for the treatment of the different hard-to-treat infections, such as bloodstream infections and catheter- or implant-related infections.

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“…The red fluorescence as depicted in Figure 11 demonstrates a higher uptake of Rhodamine B CS-LIPO in cells, indicating augmented penetration of the liposomes mainly in the cytoplasm region and more intensely in the nucleus owing to positively charged chitosan coating that could uphold endocytic cellular uptake via cell membrane interaction and the small size of liposomes lying in the nanometric range resulting in immense incorporation inside the cells. 36 The higher uptake displayed by rhodamine B-loaded CS-LIPO is anticipated to result in a more efficient antitumor action. However, plain rhodamine B dye in the rhodamine B solution cell was incapable of penetrating the MCF-7 cells.…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%

Novel Chitosan-Coated Liposomes Coloaded with Exemestane and Genistein for an Effective Breast Cancer Therapy

Sharma,

Gupta,

Kawish

et al. 2024

ACS Omega

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For achieving high effectiveness in the management of breast cancer, coadministration of drugs has attracted a lot of interest as a mode of therapy when compared to a single chemotherapeutic agent that often results in reduced therapeutic end results. Owing to their proven effectiveness, good patient compliance, and lower costs, oral anticancer drugs have received much attention. In the present work, we formulated the chitosan-coated nanoliposomes loaded with two lipophilic agents, namely, exemestane (EXE) and genistein (GEN). The formulation was prepared using the ethanol injection method, which is considered a simple method for getting the nanoliposomes. The formulation was optimized using Box−Behnken design (BBD) and was extensively characterized for particle size, ζ-potential, Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), and Xray diffraction (XRD) analysis. The sizes of conventional and coated liposomes were found to be 104.6 ± 3.8 and 120.3 ± 6.4 nm with a low polydispersity index of 0.399 and 0.381, respectively. The ζpotential of the liposomes was observed to be −16.56 mV, which changed to a positive value of +22.4 mV, clearly indicating the complete coating of the nanoliposomes by the chitosan. The average encapsulation efficiency was found to be between 70 and 80% for all prepared formulations. The compatibility of the drug with excipients and complete dispersion of the drug inside the system were verified by FTIR, XRD, and DSC studies. Furthermore, the in vitro release studies concluded the sustained release pattern following the Korsmeyer-Peppas model as the best-fitting model with Fickian diffusion. Ex vivo studies showed better permeation of the chitosan-coated liposomes, which was further confirmed by confocal studies. The prepared chitosan-coated liposomes showed superior antioxidant activity (94.56%) and enhanced % cytotoxicity (IC50 7.253 ± 0.34 μM) compared to the uncoated liposomes. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay displayed better cytotoxicity of the chitosan-coated nanoliposomes compared to the plain drug, showing the better penetration and enhanced bioavailability of drugs inside the cells. The formulation was found to be safe for administration, which was confirmed using the toxicity studies performed on an animal model. The above data suggested that poorly soluble lipophilic drugs could be successfully delivered via chitosan-coated liposomes for their effective delivery in breast cancer.

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Formulation of chitosan coated nanoliposomes for the oral delivery of colistin sulfate: in vitro characterization, ^99mTc-radiolabeling and in vivo biodistribution studies

Cited by 19 publications

References 64 publications

Active Composite Packaging Reinforced with Nisin-Loaded Nano-Vesicles for Extended Shelf Life of Chicken Breast Filets and Cheese Slices

Active Composite Packaging Reinforced with Nisin-Loaded Nano-Vesicles for Extended Shelf Life of Chicken Breast Filets and Cheese Slices

Current Advances in Lipid and Polymeric Antimicrobial Peptide Delivery Systems and Coatings for the Prevention and Treatment of Bacterial Infections

Novel Chitosan-Coated Liposomes Coloaded with Exemestane and Genistein for an Effective Breast Cancer Therapy

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