Formulation of <i>N</i>-acetylputrescine and <i>N</i>1-acetylspermidine in cultured human lymphocytes

Menashe, M.; Faber, J.; Bachrach, Uriel

doi:10.1042/bj1880263

Cited by 23 publications

(3 citation statements)

References 12 publications

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“…N ‐acetylputrescine is the acetylated form of putrescine. In humans, polyamine N ‐acetylation has been demonstrated to occur in the liver and kidneys (Menashe, Faber, & Bachrach, ). The roles of N ‐acetylation of putrescine in humans, the specific enzyme(s), and subcellular localization have not been completely characterized.…”

Section: Discussionmentioning

confidence: 99%

“…One role of N ‐acetylputrescine in humans is to serve as an alternate substrate to glutamate for oxidation to gamma ‐Aminobutyric acid (GABA) in the central nervous system (CNS) [Yoon & Lee, ]. In addition, acetylation may be involved in the inactivation of putrescine by reducing its positive charge and thus its interactions with polyanions (Menashe et al, ; Moinard et al, ). Acetylation may also facilitate increased urinary excretion of putrescine, as seen in Patient 1 on at least one point‐in‐time.…”

Section: Discussionmentioning

confidence: 99%

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Gain‐of‐function variants in the ODC1 gene cause a syndromic neurodevelopmental disorder associated with macrocephaly, alopecia, dysmorphic features, and neuroimaging abnormalities

Rodan

Anyane‐Yeboa

Wassink-Ruiter

et al. 2018

American J of Med Genetics Pt A

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Polyamines serve a number of vital functions in humans, including regulation of cellular proliferation, intracellular signaling, and modulation of ion channels. Ornithine decarboxylase 1 (ODC1) is the rate-limiting enzyme in endogenous polyamine synthesis. In this report, we present four patients with a distinct neurometabolic disorder associated with de novo heterozygous, gain-offunction variants in the ODC1 gene. This disorder presents with global developmental delay, ectodermal abnormalities including alopecia, absolute or relative macrocephaly, and characteristic facial dysmorphisms. Neuroimaging variably demonstrates white matter abnormalities, prominent Virchow-Robin spaces, periventricular cysts, and abnormalities of the corpus callosum.Plasma clinical metabolomics analysis demonstrates elevation of N-acetylputrescine, the acetylated form of putrescine, with otherwise normal polyamine levels. Therapies aimed at reducing putrescine levels, including ODC1 inhibitors, dietary interventions, and antibiotics to reduce polyamine production by gastrointestinal flora could be considered as disease-modifying therapies. As the ODC1 gene has been implicated in neoplasia, cancer surveillance may be important in this disorder. K E Y W O R D S alopecia, neurodevelopmental disorder, ornithine decarboxylase 1 (ODC1), polyamines, putrescine

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gain‐of‐function variants in the ODC1 gene cause a syndromic neurodevelopmental disorder associated with macrocephaly, alopecia, dysmorphic features, and neuroimaging abnormalities

Rodan

Anyane‐Yeboa

Wassink-Ruiter

et al. 2018

American J of Med Genetics Pt A

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“…known. It is known, however, that putrescine and spermidine undergo monoacetylation in cultured human lymphocytes (Menashe et al, 1980).…”

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confidence: 99%

Response of enzymes involved in the metabolism of polyamines to phytohaemagglutinin-induced activation of human lymphocytes

Korpela¹,

Hölttä²,

Hovi³

et al. 1981

Biochemical Journal

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The stimulation of lymphocyte ornithine decarboxylase and adenosylmethionine decarboxylase produced by phytohaemagglutinin was accompanied by an equally marked, but delayed, stimulation of spermidine synthase, which is not commonly considered as an inducible enzyme. In contrast with the marked stimulation of these biosynthetic enzymes, less marked changes were observed in the biodegradative enzymes of polyamines in response to phytohaemagglutinin. Diamine oxidase activity was undetectable during all stages of the transformation. The activity of polyamine oxidase remained either constant or was slightly decreased several days after addition of the mitogen. The activity of polyamine acetylase (employing all the natural polyamines as substrates) distinctly increased both in the cytosolic and crude nuclear preparations of the cells during later stages of mitogen activation. Difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, although powerfully inhibiting ornithine decarboxylase, produced a gradual enhancement of adenosylmethionine decarboxylase activity during lymphocyte activation, without influencing the activities of the two propylamine transferases (spermidine synthase and spermine synthase).

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Occurrence in high concentrations of N1-acetylspermidine and sym-homospermidine in the hamster epididymis

Matsuzaki

Hamana

Imai

et al. 1982

Biochemical and Biophysical Research Communications

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Formulation of N-acetylputrescine and N1-acetylspermidine in cultured human lymphocytes

Cited by 23 publications

References 12 publications

Gain‐of‐function variants in the ODC1 gene cause a syndromic neurodevelopmental disorder associated with macrocephaly, alopecia, dysmorphic features, and neuroimaging abnormalities

Gain‐of‐function variants in the ODC1 gene cause a syndromic neurodevelopmental disorder associated with macrocephaly, alopecia, dysmorphic features, and neuroimaging abnormalities

Response of enzymes involved in the metabolism of polyamines to phytohaemagglutinin-induced activation of human lymphocytes

Occurrence in high concentrations of N1-acetylspermidine and sym-homospermidine in the hamster epididymis

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