Formulation of PLGA nano-carriers: specialized modification for cancer therapeutic applications

Chatterjee, Manosree; Chanda, Nripen

doi:10.1039/d1ma00600b

Cited by 35 publications

(14 citation statements)

References 186 publications

(198 reference statements)

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“…The pH of the environment is critical for oxygen release studies since the OH ions may interact with the components of the shell of ONBs to result in a change in zeta potential, 24 , 25 and, therefore, its physical properties. The oxygen release characteristics of ONBs was evaluated in various pH conditions to assess stability, and the results are shown in Figure 3 B.…”

Section: Resultsmentioning

confidence: 99%

“… 21 – 23 Polymers have been used for NB shell formulation because of their tunable molecular weight, polymer type, and functionalization that can enhance specific properties of the bubbles. 21 , 25 Proteins used as NB shells are more stable than lipid shells and are able to self-assemble under specific circumstances. 21 NBs with protein shells also allow for limited diffusion, 22 providing a constant release of a therapeutic gas.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in Hypoxia

Messerschmidt

Ren

Tsipursky

et al. 2023

Trans. Vis. Sci. Tech.

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Purpose Vein or artery occlusion causes a hypoxic environment by preventing oxygen delivery and diffusion to tissues. Diseases such as retinal vein occlusion, central retinal artery occlusion, or diabetic retinopathy create a stroke-type condition that leads to functional blindness in the effected eye. We aim to develop an oxygen delivery system consisting of oxygen nanobubbles (ONBs) that can mitigate retinal ischemia during a severe hypoxic event such as central retinal artery occlusion. Methods ONBs were synthesized to encapsulate oxygen saturated molecular medical grade water. Stability, oxygen release, biocompatibility, reactive oxygen species, superoxide, MTT, and terminal uridine nick-end labeling assays were performed. Cell viability was evaluated, and safety experiments were conducted in rabbits. Results The ONBs were approximately 220 nm in diameter, with a zeta potential of −58.8 mV. Oxygen release studies indicated that 74.06 µg of O 2 is released from the ONBs after 12 hours at 37°C. Cell studies indicated that ONBs are safe and cells are viable. There was no significant increase in reactive oxygen species, superoxide, or double-stranded DNA damage after ONB treatment. ONBs preserve mitochondrial function and viability. Histological sections from rabbit eyes indicated that ONBs were not toxic. Conclusions The ONBs proposed have excellent oxygen holding and release properties to mitigate ischemic conditions in the retina. They are sterile, stable, and nontoxic. Translation Relevance ONB technology was evaluated for its physical properties, oxygen release, sterility, stability, and safety. Our results indicate that ONBs could be a viable treatment approach to mitigate hypoxia during ischemic conditions in the eye upon timely administration.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in Hypoxia

Messerschmidt

Ren

Tsipursky

et al. 2023

Trans. Vis. Sci. Tech.

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“…DOX was encapsulated with nanosized spherical PLGA via noncovalent interactions. [ 29 ] To examine the presence of tumor extracellular vesicles (EVs) on the nanostemsome, EVs were isolated and stained in the conditioned media of tumor cells (Figure S4, Supporting Information). The EV concentration in the conditioned media used in the existing education process was set to 1× (normal concentration), while 8× (very high) concentration was used as the positive control (Figure S4, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Pancreatic Tumor‐Targeting Stemsome Therapeutics

Park

Jeong

et al. 2023

Advanced Materials

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Owing to the intrinsic ability of stem cells to target the tumor environment, stem‐cell‐membrane‐functionalized nanocarriers can target and load active anticancer drugs. In this work, a strategy that focuses on stem cells that self‐target pancreatic cancer cells is developed. In particular, malignant deep tumors such as pancreatic cancer cells, one of the intractable tumors that currently have no successful clinical strategy, are available for targeting and destruction. By gaining the targeting ability of stem cells against pancreatic tumor cells, stem cell membranes can encapsulate nano‐polylactide‐co‐glycolide loaded with doxorubicin to target and reduce deep pancreatic tumor tissues. Considering the lack of known target proteins on pancreatic tumor cells, the suggested platform technology can be utilized for targeting any malignant tumors in which surface target receptors are unavailable.

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“…These features make polymers appropriate materials for diverse bio-applications [17][18][19]. To this end, polymeric NPs made up of poly(lactic-co-glycolic acid) (PLGA) have been widely used in biomedical research owing to their biocompatible and biodegradable properties [20] necessary to transport not only small molecule drugs but also peptides and macromolecules [21][22][23]. Interestingly, PLGA degradation rates can also be modulated depending on both molecular weight and the copolymer ratio.…”

Section: Introductionmentioning

confidence: 99%

Rosmarinic Acid-Loaded Polymeric Nanoparticles Prepared by Low-Energy Nano-Emulsion Templating: Formulation, Biophysical Characterization, and In Vitro Studies

et al. 2022

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Rosmarinic acid (RA), a caffeic acid derivative, has been loaded in polymeric nanoparticles made up of poly(lactic-co-glycolic acid) (PLGA) through a nano-emulsion templating process using the phase-inversion composition (PIC) method at room temperature. The obtained RA-loaded nanoparticles (NPs) were colloidally stable exhibiting average diameters in the range of 70–100 nm. RA was entrapped within the PLGA polymeric network with high encapsulation efficiencies and nanoparticles were able to release RA in a rate-controlled manner. A first-order equation model fitted our experimental data and confirmed the prevalence of diffusion mechanisms. Protein corona formation on the surface of NPs was assessed upon incubation with serum proteins. Protein adsorption induced an increase in the hydrodynamic diameter and a slight shift towards more negative surface charges of the NPs. The radical scavenging activity of RA-loaded NPs was also studied using the DPPH·assay and showed a dose–response relationship between the NPs concentration and DPPH inhibition. Finally, RA-loaded NPs did not affect the cellular proliferation of the human neuroblastoma SH-SY5Y cell line and promoted efficient cellular uptake. These results are promising for expanding the use of O/W nano-emulsions in biomedical applications.

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Formulation of PLGA nano-carriers: specialized modification for cancer therapeutic applications

Abstract: The eminence of nano-scale materials prevailed after the invention of high-resolution microscopes. Nowadays, nanoparticles are predominantly found in every application, including biomedical applications. In nanomedicine, the unique properties make nano-scale...

Cited by 35 publications

References 186 publications

Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in Hypoxia

Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in Hypoxia

Pancreatic Tumor‐Targeting Stemsome Therapeutics

Rosmarinic Acid-Loaded Polymeric Nanoparticles Prepared by Low-Energy Nano-Emulsion Templating: Formulation, Biophysical Characterization, and In Vitro Studies

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