Over the past ten years, nanoscale science and technology have become revolutionary areas of study. Due to their ability to withstand challenging processing circumstances, inorganic materials like metal oxides and metals have received an attention in the past 10 years. Metal oxides like zinc oxide, calcium oxide, titanium dioxide, and magnesium oxide are important inorganic materials that are generally considered safe for use by humans and animals. Due to their antibacterial qualities, zinc oxide and silver nanoparticles have been used as an effective approach for preventing infectious infections. Shape, crystallinity, composition, and morphology are the primary determinants of the intrinsic properties of metal nanoparticles. In this research, sodium hydroxide, zinc nitrate and soluble starch were used as precursors and stabilising agents in the chemical creation of zinc oxide nanoparticles. Then zinc oxide nanoparticles were characterized for evaluation of their properties. The ZnO nanoparticle assay, TEM, UV-visible spectroscopy, SEM, EDX, antimicrobial test, and Malvern size distribution report were used to characterize the zinc oxide nanoparticles. The average particle size of zinc oxide nanoparticles was found to be 10-40 nm. The antibacterial activity of the zinc oxide nanoparticles was demonstrated against Candida albicans (CA), Escherichia coli (EC), Staphylococcus aureus (SA), and Pseudomonas aeruginosa (PA). During comparison of zone of inhibition it was observed that the zinc oxide nanoparticles show strong antimicrobial activity than zinc oxide powder.