Formulation Strategies for Folate-Targeted Liposomes and Their Biomedical Applications

Kumar, Parveen; Huo, Peipei; Liu, Bo

doi:10.3390/pharmaceutics11080381

Cited by 81 publications

(59 citation statements)

References 162 publications

(165 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The functionalization of the liposomes surface with different ligands, such as peptides, monoclonal antibodies, aptamers, growth factors, sensitively ameliorates the specificity of the liposome interaction with target sites (targeted drug delivery) [176,177]. Folate receptor (FR) is one of the most commonly employed tumor-associated antigens that can bind (with high affinity) with folic acid (FA) and its conjugates and is able to ingest the bound molecules inside the targeted cells via the endocytic pathway [178]. Folate ligands have been largely employed for the delivery, to FR-overexpressed cells, of different anticancer drugs (including Doxorubricin, Methotrexate, Vincristine, Paclitaxel), up to large macromolecules containing DNA, which proved to be highly effective as targeted drug delivery system [178].…”

Section: Lipidsmentioning

confidence: 99%

“…Folate receptor (FR) is one of the most commonly employed tumor-associated antigens that can bind (with high affinity) with folic acid (FA) and its conjugates and is able to ingest the bound molecules inside the targeted cells via the endocytic pathway [178]. Folate ligands have been largely employed for the delivery, to FR-overexpressed cells, of different anticancer drugs (including Doxorubricin, Methotrexate, Vincristine, Paclitaxel), up to large macromolecules containing DNA, which proved to be highly effective as targeted drug delivery system [178]. For example, folate-conjugated liposomes evidenced an efficient internalization (though FR-α-mediated endocytosis) in H1299 cells.…”

Section: Lipidsmentioning

confidence: 99%

“…For example, folate-conjugated liposomes evidenced an efficient internalization (though FR-α-mediated endocytosis) in H1299 cells. The induced apoptosis together with cell-cycle arrest favored a sensitive growth inhibition process [178].…”

Section: Lipidsmentioning

confidence: 99%

See 2 more Smart Citations

Self-Assembly of Organic Nanomaterials and Biomaterials: The Bottom-Up Approach for Functional Nanostructures Formation and Advanced Applications

et al. 2020

View full text Add to dashboard Cite

In this paper, we survey recent advances in the self-assembly processes of novel functional platforms for nanomaterials and biomaterials applications. We provide an organized overview, by analyzing the main factors that influence the formation of organic nanostructured systems, while putting into evidence the main challenges, limitations and emerging approaches in the various fields of nanotechology and biotechnology. We outline how the building blocks properties, the mutual and cooperative interactions, as well as the initial spatial configuration (and environment conditions) play a fundamental role in the construction of efficient nanostructured materials with desired functional properties. The insertion of functional endgroups (such as polymers, peptides or DNA) within the nanostructured units has enormously increased the complexity of morphologies and functions that can be designed in the fabrication of bio-inspired materials capable of mimicking biological activity. However, unwanted or uncontrollable effects originating from unexpected thermodynamic perturbations or complex cooperative interactions interfere at the molecular level with the designed assembly process. Correction and harmonization of unwanted processes is one of the major challenges of the next decades and requires a deeper knowledge and understanding of the key factors that drive the formation of nanomaterials. Self-assembly of nanomaterials still remains a central topic of current research located at the interface between material science and engineering, biotechnology and nanomedicine, and it will continue to stimulate the renewed interest of biologist, physicists and materials engineers by combining the principles of molecular self-assembly with the concept of supramolecular chemistry.

show abstract

Section: Lipidsmentioning

confidence: 99%

Section: Lipidsmentioning

confidence: 99%

See 1 more Smart Citation

Self-Assembly of Organic Nanomaterials and Biomaterials: The Bottom-Up Approach for Functional Nanostructures Formation and Advanced Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The delivery of cargo in non-targeted liposomes is mediated by the EPR effect [205]. Nevertheless, in the last years, targeted liposomal formulations have been described and particularly folate-conjugated liposomes that involves the attachment of folic acid with phospholipids, cholesterol or peptides before liposomal formulation synthesis has been developed (for a review see [206]). The most common application of liposomes in gene delivery is to carry DNA into the cells [22].…”

Section: Lipid Basedmentioning

confidence: 99%

Gene Therapy in Cancer Treatment: Why Go Nano?

et al. 2020

View full text Add to dashboard Cite

The proposal of gene therapy to tackle cancer development has been instrumental for the development of novel approaches and strategies to fight this disease, but the efficacy of the proposed strategies has still fallen short of delivering the full potential of gene therapy in the clinic. Despite the plethora of gene modulation approaches, e.g., gene silencing, antisense therapy, RNA interference, gene and genome editing, finding a way to efficiently deliver these effectors to the desired cell and tissue has been a challenge. Nanomedicine has put forward several innovative platforms to overcome this obstacle. Most of these platforms rely on the application of nanoscale structures, with particular focus on nanoparticles. Herein, we review the current trends on the use of nanoparticles designed for cancer gene therapy, including inorganic, organic, or biological (e.g., exosomes) variants, in clinical development and their progress towards clinical applications.

show abstract

“…FA can be easily internalized in a nondestructive manner into cancerous cells via endocytic pathway that facilitates delivery of folate-conjugated anticancer drug payloads into cells in the same receptormediated process called a Trojan horse strategy (9)(10)(11). To date, treatments that involve folate-targeted approaches were extensively studied in context of variety of therapeutic cargos, including chemotherapeutic agents, imaging agents, oligonucleotides and DNA molecules (12). One of the widely used chemotherapeutic agent is doxorubicin, a topoisomerase inhibitor that interaction with DNA leads to growth suppression of cancer cells (13).…”

Section: Introductionmentioning

confidence: 99%

Quantum dots as targeted doxorubicin drug delivery nanosystems in human lung cancer cells

Ruzycka-Ayoush

Kowalik

Kowalczyk

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Lung cancer is one of the most frequently diagnosed cancer all over the world and a leading cancer-related mortality. The therapy of lung cancer includes surgery, chemotherapy and radiatherapy and mailny depends on the type and stage of lung cancer characterized based on WHO guidelines. Althought the conventional chemotherapy is the main treatment option for small cell lung cancer (SCLC) and a common treatment for non-SCLC it is characterized with lack of specificity resulting to severe toxicities of normal cells and harmful side effects. Therefore, targeted drug delivery (TDD) systems have been used to reduce the systemic toxicity of some conventional chemotherapies in lung cancer. Quantum dots (QDs) are fascinating nanoscale crystals that can serve as nanocarriers in TDD due to their unique physicochemical properties. Therefore, in this paper, the as-desiged QDs, Ag-In-Zn-S-based nanoconjugates for selective doxorubicin (DOX) targeting to lung cancer cells were developed. The QD nanocrystals were modified with 11-mercaptoundecanoic acid (MUA), L-cysteine (Cys) and lipoic acid (LA) used as drug carriers for targeted delivery of DOX to A549 cells through conjugated folic acid (FA) a self-navigation molecule that docks to the folate receptors on cancer cells. The comprechensive physicochemical, cytotoxicity and genotoxicity studies were performed to characterise the novel QD-based nanocaries and their anticancer cargos. Results The results from FTIR, DLS and fluorescence quenching evidenced the successful attachment of FA to the QDs nanocrystals and DOX to the QDs-FA nanocarriers. UV-vis analysis determined the amount of FA and DOX covalently anchored to the QDs nanocrystal surface. Biological screeining revealed that QDs-FA-DOX nanoconjugates showed higher cytotoxicity in comparison to other forms of the synthesized QD samples, suggesting the cytotoxic effect of liberated DOX from the QD constructs. QD-MUA-FA-DOX occurred to be the most cytotoxic against A549 cells among nanoconjugates. In vitro scratch assay also revealed significant inhibition of A549 migration only due to treatment with QD-MUA-FA-DOX. Studies evidenced that all the nanoconjugates at IC 50 induced significantly more DNA breaks than that observed in non-treated cells. All in all, significant and the greatest cytotoxicity, genotoxicity together with inhibition of migratory potential of A549 cells was observed for QD-MUA-FA-DOX. Conclusion The studies show the therapeutic efficacy of DOX-loaded QD-based cargos suggesting their promising role as novel drug delivery systems navigating to folate receptors in lung cancer cells.

show abstract

Formulation Strategies for Folate-Targeted Liposomes and Their Biomedical Applications

Cited by 81 publications

References 162 publications

Self-Assembly of Organic Nanomaterials and Biomaterials: The Bottom-Up Approach for Functional Nanostructures Formation and Advanced Applications

Self-Assembly of Organic Nanomaterials and Biomaterials: The Bottom-Up Approach for Functional Nanostructures Formation and Advanced Applications

Gene Therapy in Cancer Treatment: Why Go Nano?

Quantum dots as targeted doxorubicin drug delivery nanosystems in human lung cancer cells

Contact Info

Product

Resources

About