Forskolin potentiates adrenocorticotropin-induced cyclic AMP production and steroidogenesis in isolated rat adrenal cells

Moriwaki, Kaname; Itoh, Yoshiharu; Iida, Shinji; Ichihara, K.

doi:10.1016/0024-3205(82)90299-5

Cited by 38 publications

(8 citation statements)

References 8 publications

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“…In similar experiments incorporating a maximally effective concentration (10-SM) of cholera toxin to test the cAMP and PTH response in six human adenomas at low and high Ca 2+ in 2 h incubations, we again found no stimulatory effect of cholera toxin upon PTH secretion, despite marked stimulation (up to 85-fold) of cAMP levels at 2 h [20]. In contrast, release of corticosteroids from mouse adrenal Y-1 tumor cells (data not shown) and rat [14] adrenal cells did appear to reflect changes in cAMP produced by forskolin and ACTH, thus confirming that the stimulating effects of forskolin upon adenylate cyclase can lead to hormone secretion in other tissues.…”

Section: Discussioncontrasting

confidence: 36%

“…Adenylate cyclase activity in membrane preparations from human parathyroid adenomas was stimulated markedly by forskolin, as has been reported in other tissues [6,14]. Table 1 shows a representative experiment in which a clear-cut dose-response relation between enzyme activity and forskolin occurred, with significant stimulation over basal activity occurring at a 1 ~M concentration (lowest dose tested), and a maximal 13-fold rise occurring when 100 p,M forskolin was used (P < .001).…”

Section: Effects Of Forskolin On Parathyroid Adenylate Cyclasementioning

confidence: 53%

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Divergent effects of forskolin on 3′,5′ cyclic adenosine monophosphate production and parathyroid hormone secretion

et al. 1984

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Forskolin, a diterpene which directly stimulates adenylate cyclase, markedly stimulated cAMP production in intact rat parathyroid glands and dispersed cells from hyperplastic and adenomatous human parathyroid tissues. Stimulation of cAMP production in human parathyroid adenomas occurred as early as 2 min and continued for at least 2 h; furthermore, a dose-response relationship was observed, with a maximal 80-fold cAMP response occurring at 100 microM forskolin. When PTH secretion by rat or human parathyroid tissues was studied at low (0.5 mM) and high (2.5 mM) extracellular Ca2+ in either the presence or absence of forskolin, no significant stimulation by forskolin was observed at 15 min, 1 h, and 2 h. When 10 human parathyroid specimens were studied with varying concentrations of forskolin at 1 mM Ca2+, 6 failed to show stimulation of PTH secretion and 4 showed modest but detectable increases in PTH that did not appear dose-related. We conclude that (1) at low and high Ca2+ levels, marked stimulation of cAMP production by forskolin can occur without a corresponding increase in PTH secretion; (2) inhibition of PTH secretion by high extracellular Ca2+ levels continues unchanged despite stimulation of cAMP production by forskolin; and (3) at intermediate Ca2+ levels (1.0 mM), PTH secretion is affected either minimally or not at all by forskolin in human hyperparathyroid tissue preparations. The marked stimulation of parathyroid adenylate cyclase by forskolin without concomitant increases in PTH secretion in the majority of tissues suggests that the level of cAMP production is not a primary or sufficient determinant of hormone secretion.

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Section: Discussioncontrasting

confidence: 36%

Section: Effects Of Forskolin On Parathyroid Adenylate Cyclasementioning

confidence: 53%

Divergent effects of forskolin on 3′,5′ cyclic adenosine monophosphate production and parathyroid hormone secretion

et al. 1984

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“…In the present study, in order to determine whether procaine has influence on steroidogenesis via cyclic AMP or not, effects of procaine on (Bu)2 cAMP-, forskol.in and Ca2+-induced ste roidogenesis were examined by using of isolated bovine adrenocortical cells. It was reported that forskolin increases intracellular cyclic AMP levels and stimulated steroid pro duction in rat adrenal cells (10) and rat ovarian tissue (11).…”

Section: Abstract-effectsmentioning

confidence: 99%

Procaine inhibits cyclic AMP-induced steroidogenesis in isolated bovine adrenocortical cells.

et al. 1990

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Abstract-Effectsof procaine on dibutyryl adenosine 3',5'-cyclic monophosphate ((Bu)2cAMP)-, Ca t+ or forskolin-induced steroidogenesis were examined in isolated bovine adrenocortical cells. Procaine (<1.0 mM) caused a marked sup pression of (Bu)2cAMP or forskolin-induced steroidogenesis in the absence of extracellular Cat', but did not affect on Cat+-induced steroidogenesis in the cells. (Bu)2cAMP decreased the cell associated 45Ca2+. However, procaine (300 fM) inhibited this effect of (Bu)2cAMP.These results suggest that procaine may abolish (Bu)2cAMP-induced Ca2+ release from intracellular calcium store(s) and inhibits steroidogenesis.

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“…In addition, forskolin elicits physiological responses generally thought to be linked to hormone-elicited elevations of cAMP (Moriwaki et al, 1982;Hersey et al, 1983;Takahashi et al, 1983). It has been demonstrated that forskolin stimulates thyroid adenylate cyclase, cAMP accumulation and thyroid hormone secretion (Fradkin et al, 1982;Van Sande et al, 1983;Totsuka et al, 1983;Laurberg, 1984).…”

Section: Discussionmentioning

confidence: 99%

Forskolin stimulation of 3,5,3'-triiodothyronine release from perifused rat thyroids.

et al. 1985

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Forskolin potentiates adrenocorticotropin-induced cyclic AMP production and steroidogenesis in isolated rat adrenal cells

Cited by 38 publications

References 8 publications

Divergent effects of forskolin on 3′,5′ cyclic adenosine monophosphate production and parathyroid hormone secretion

Divergent effects of forskolin on 3′,5′ cyclic adenosine monophosphate production and parathyroid hormone secretion

Procaine inhibits cyclic AMP-induced steroidogenesis in isolated bovine adrenocortical cells.

Forskolin stimulation of 3,5,3'-triiodothyronine release from perifused rat thyroids.

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