Forssman Antibody Levels in Sera of Cancer Patients

Mori, Tadashige; Fujii, G; Kawamura, Akitoshi; Yasuda, Tatsuji; Naito, Yuko; Tsumita, T

doi:10.3109/08820138209094132

Cited by 15 publications

(14 citation statements)

References 10 publications

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“…[7][8][9][10][11][12][13] We previously examined the presence/absence of activating missense mutations at codon 230 or 296 of the human GBGT1 gene in the Catalogue of Somatic Mutations in Cancer Database. However, no such mutations were found, although the database lists 60 mutations in the GBGT1 gene in various cancers.…”

Section: Discussionmentioning

confidence: 99%

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ABO blood group A transferases catalyze the biosynthesis of FORS blood group FORS1 antigen upon deletion of exon 3 or 4

2017

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Key Points• ABO blood group A transferases possess intrinsic FS activity upon deletion of exon 3 or 4 of A transferase messenger RNAs.• Cointroduction of exon 3 or 4 deletion and GlyGlyAla substitution synergistically confers human A transferases with strong FS activity. (B3GALNT1) cells, and cell-surface expression of FORS1 was immunologically monitored. To our surprise, the deletion of exon 3 or 4, but not of exon 2 or 5, of human AT transcripts bestowed moderate FS activity, indicating that the A allele is inherently capable of producing a protein with FS activity. Because RNA splicing is frequently altered in cancer, this mechanism may explain, at least partially, the appearance of FORS1 in human cancer. Furthermore, strong FS activity was attained, in addition to AT and BT activities, by cointroducing 1 of those deletions and the GlyGlyAla substitution, possibly by the synergistic effects of altered intraGolgi localization/conformation by the former and modified enzyme specificity by the latter.

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Section: Discussionmentioning

confidence: 99%

“…5,6 In addition to occurring in A pae individuals, FORS1 expression has also been reported in cancer cells/tissues from non-A pae individuals. [7][8][9][10][11][12][13] However, the molecular mechanisms that induce heterophilic FORS1 expression remained to be determined.…”

Section: Introductionmentioning

confidence: 99%

ABO blood group A transferases catalyze the biosynthesis of FORS blood group FORS1 antigen upon deletion of exon 3 or 4

2017

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“…Forssman antibodies, has been documented earlier [19,20] . In our study, the postoperative elevation of antibody levels was found to be related to the low-differentiated carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients

Smorodin¹,

Kurtenkov²,

Sergeyev³

et al. 2008

WJG

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AIM:To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to followup. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cutoff value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ 2 = 3.89, all patients; χ 2 = 5.34, patients without regional lymph node metastases; P < 0. No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stageand morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens.

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“…The spe cificity of the corresponding antigen was shown to be dependent on the GalNac-GalNac portion of glycolipid of F-positive erythrocyte membrane [61] or the same two terminal sugars of glycoprotein isolated from tissues of F-positive species [62]. F-antibody ti ters in normal human sera vary from one individual to another [63] and they tend to increase in patients with carcinomas of the gastrointestinal tract after ra dical surgery [64], Significantly F antigen has been demonstrated in and isolated from various carcino mas [65][66][67][68], The mechanism of expression of F anti gen in malignancies is now being investigated [68,69], Finally, our recent studies have shown that agglu tinins against Victoria strain of NDV-treated group O red blood cells found in IM and other pathologic sera as well as some of antibodies to the NDV preparation are directed to the antigen of the hemagglutinin-neu raminidase subunit of the NDV [38][39][40], Interestingly, the antigen of the hemagglutinin-neuraminidase molecule recognized by these pathologic sera is also present in GPK tissues [38][39][40], Thus the NDV anti bodies were now classified into the GPK-positive group of human heterophile antibodies. …”

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confidence: 99%