FÖrster resonance energy transfer (FRET)-based biosensors for biological applications

Zhang, Xiaojing; Hu, Yue; Yang, Xiaotong; Tang, Yingying; Han, Seung Hoon; An, Kang; Deng, Haishan; Chi, Yumei; Zhu, Dong; Lu, Yin

doi:10.1016/j.bios.2019.05.019

Cited by 185 publications

(95 citation statements)

References 151 publications

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“…Further, the enrichment of live cells can circumvent errors due to synchronization. The range of available FRET-based probes for kinase/phosphatase balances is rapidly increasing [28], and the last two decades have seen a rise in various cellular pathways studied by FRET-based reporters [29,30]. FRET-activated cell sorting (FRACS) could therefore be used to study a range of rare events.…”

Section: Discussionmentioning

confidence: 99%

FRET-Based Sorting of Live Cells Reveals Shifted Balance between PLK1 and CDK1 Activities During Checkpoint Recovery

Lafranchi

Müllers

Rutishauser

et al. 2020

Cells

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Cells recovering from the G2/M DNA damage checkpoint rely more on Aurora A-PLK1 signaling than cells progressing through an unperturbed G2 phase, but the reason for this discrepancy is not known. Here, we devised a method based on a FRET reporter for PLK1 activity to sort cells in distinct populations within G2 phase. We employed mass spectroscopy to characterize changes in protein levels through an unperturbed G2 phase and validated that ATAD2 levels decrease in a proteasome-dependent manner. Comparing unperturbed cells with cells recovering from DNA damage, we note that at similar PLK1 activities, recovering cells contain higher levels of Cyclin B1 and increased phosphorylation of CDK1 targets. The increased Cyclin B1 levels are due to continuous Cyclin B1 production during a DNA damage response and are sustained until mitosis. Whereas partial inhibition of PLK1 suppresses mitotic entry more efficiently when cells recover from a checkpoint, partial inhibition of CDK1 suppresses mitotic entry more efficiently in unperturbed cells. Our findings provide a resource for proteome changes during G2 phase, show that the mitotic entry network is rewired during a DNA damage response, and suggest that the bottleneck for mitotic entry shifts from CDK1 to PLK1 after DNA damage.

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Section: Discussionmentioning

confidence: 99%

FRET-Based Sorting of Live Cells Reveals Shifted Balance between PLK1 and CDK1 Activities During Checkpoint Recovery

Lafranchi

Müllers

Rutishauser

et al. 2020

Cells

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“…Förster RET (FRET) involves energy transfer from an excited molecular chromophore (the donor) to another chromophore (the acceptor). 107,108 The following factors are primarily required to cause FRET: (i) a spectral overlap between the uorescence of the donor chromophore and the absorption of the acceptor chromophore, (ii) small distance between the donor and the acceptor (typically, 10-100Å), and (iii) parallel orientations of the donor and the acceptor transition dipoles. Yamamoto and Kawasaki et al applied FRET for a nanocomposite of BSA-Au NC and methylene blue (MB) (BSA-Au NC-MB) to enhance the 1 O 2 generation.…”

Section: Basic Principle Of Photosensitization For Pdtmentioning

confidence: 99%

Photo/electrocatalysis and photosensitization using metal nanoclusters for green energy and medical applications

2020

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“…Our data demonstrated that MNPs act as Förster resonance energy transfer (FRET)-based quenchers (acceptors) because of their broad energy absorption bandwidth in the visible range which overlaps with the fluorescence emission spectrum of PheoA (donor) [ 35 , 36 , 37 ]. Therefore, the photodynamic design of MNPs inevitably requires a specific type of dequenching system, such as a cancer redox-responsive cleavable system.…”

Section: Resultsmentioning

confidence: 99%

T1-Positive Mn2+-Doped Multi-Stimuli Responsive poly(L-DOPA) Nanoparticles for Photothermal and Photodynamic Combination Cancer Therapy

et al. 2020

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In this study, we designed near-infrared (NIR)-responsive Mn2+-doped melanin-like poly(L-DOPA) nanoparticles (MNPs), which act as multifunctional nano-platforms for cancer therapy. MNPs, exhibited favorable π-π stacking, drug loading, dual stimuli (NIR and glutathione) responsive drug release, photothermal and photodynamic therapeutic activities, and T1-positive contrast for magnetic resonance imaging (MRI). First, MNPs were fabricated via KMnO4 oxidation, where the embedded Mn2+ acted as a T1-weighted contrast agent. MNPs were then modified using a photosensitizer, Pheophorbide A, via a reducible disulfide linker for glutathione-responsive intracellular release, and then loaded with doxorubicin through π-π stacking and hydrogen bonding. The therapeutic potential of MNPs was further explored via targeted design. MNPs were conjugated with folic acid (FA) and loaded with SN38, thereby demonstrating their ability to bind to different anti-cancer drugs and their potential as a versatile platform, integrating targeted cancer therapy and MRI-guided photothermal and chemotherapeutic therapy. The multimodal therapeutic functions of MNPs were investigated in terms of T1-MR contrast phantom study, photothermal and photodynamic activity, stimuli-responsive drug release, enhanced cellular uptake, and in vivo tumor ablation studies.

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FÖrster resonance energy transfer (FRET)-based biosensors for biological applications

Cited by 185 publications

References 151 publications

FRET-Based Sorting of Live Cells Reveals Shifted Balance between PLK1 and CDK1 Activities During Checkpoint Recovery

FRET-Based Sorting of Live Cells Reveals Shifted Balance between PLK1 and CDK1 Activities During Checkpoint Recovery

Photo/electrocatalysis and photosensitization using metal nanoclusters for green energy and medical applications

T1-Positive Mn2+-Doped Multi-Stimuli Responsive poly(L-DOPA) Nanoparticles for Photothermal and Photodynamic Combination Cancer Therapy

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