Fortimicins A and B, new aminoglycoside antibiotics. II. Isolation, physico-chemical and chromatographic properties.

Abstract

Thin-layer chromatography of the mixed neomycin sulfates on carbon plates. Anal.

Fortimicin A: collaborative in vitro susceptibility. Comparison with amikacin and gentamicin against 11,840 clinical bacterial isolates

“…The results of these studies are also'shown in Table 4. DISCUSSION Fortimicin A and B are novel new disaccharide aminoglycosides produced by M. olivoasterospora (2,5,10,11). The structural qualities of fortimicin A protect it from most commonly encountered aminoglycoside-inactivating enzymes (10,12).…”

“…Fortamine cyclitol-containing antimicrobials were obtained from Micromonospora olivoasterospora (2,10,11). The most promising compound, fortimicin A (XK-70-1), is a novel pseudodisaccharide antibiotic with chiro cyclitol stereochemistry ( Fig.…”

Structure of fortimicin B

“…Fortimicin B is a member of the fortimicins, which are potent broad-spectrum aminocyclitol antibiotics produced by a strain of Micromonospora olivoasterospona Okachi et aL, 1977). X-ray structure determination of the title molecule was carried out in order to confirm the structure proposed from chemical and spectroscopic evidence (Egan et al, 1977) and to determine the absolute configuration unequivocally.…”

Biotransformation of Sisomicin and Verdamicin byMicromonospora sagamiensis

“…Biotransformation products were detected by means of carbon- 7 ) and silica gel-thin layer chromatography (TLC) and paper chromatography using the following solvent systems: the lower phase of chloroform-methanol-concentrated ammonium hydroxide (1: 1: 1, v/v) for silica gel-TLC, 2: 1: 1 (v/v) (17% ammonium hydroxide) for paper chromatography and 0.5 N hydrochloric acid-methanol (20: 1, v/v) for carbon TLC. Antibiotic zones on the chromatograms were detected by bioautography against Bacillus subtilis, or by Rydon-Smith reagent.…”