Forward and reverse selection for longevity in Drosophila is characterized by alteration of antioxidant gene expression and oxidative damage patterns☆

Arking, Robert; Burde, Vasanti; Graves, Kevin A.; Hari, Raj; Feldman, Elliot; Zeevi, Aaron; Soliman, Sherif; Saraiya, Ashesh A.; Buck, Steven; Vettraino, John; Sathrasala, Kalpana; Wehr, Nancy B.; Levine, Rodney L.

doi:10.1016/s0531-5565(99)00094-7

Cited by 118 publications

(71 citation statements)

References 36 publications

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“…Damage caused by reactive oxygen species, accruing over a lifetime, has been postulated to be an important determinant of life span both in normal individuals (23,24) and in animal models that show extended longevity (3,5,26). This hypothesis is supported by evidence that levels of antioxidants are higher in tissues of Ames dwarf mice (11,48), although results from GHR-KO mice are less clear (25).…”

Section: Discussionmentioning

confidence: 99%

Fibroblast cell lines from young adult mice of long-lived mutant strains are resistant to multiple forms of stress

Salmon¹,

Murakami²,

Bartke³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

226

224

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Previous studies have shown that dermal fibroblast cell lines derived from young adult mice of the long-lived Snell dwarf mutant stock are resistant, in vitro, to the cytotoxic effects of H2O2, cadmium, UV light, paraquat, and heat. We show here that similar resistance profiles are seen in fibroblast cells derived from a related mutant, the Ames dwarf mouse, and that cells from growth hormone receptor-null mice are resistant to H2O2, paraquat, and UV but not to cadmium. Resistance to UV light, cadmium, and H2O2are similar in cells derived from 1-wk-old Snell dwarf or normal mice, and thus the resistance of cell lines derived from young adult donors reflects developmental processes, presumably hormone dependent, that take place in the first few months of life. The resistance of cells from Snell dwarf mice to these stresses does not reflect merely antioxidant defenses: dwarf-derived cells are also resistant to the DNA-alkylating agent methyl methanesulfonate. Furthermore, inhibitor studies show that fibroblast resistance to UV light is unaffected by the antioxidants ascorbic acid and N-acetyl-l-cysteine. These data suggest that postnatal exposure to altered levels of pituitary hormones leads to development of cellular resistance to oxidative and nonoxidative stressors, which are stable through many rounds of in vitro cell division and could contribute to the remarkable disease resistance of long-lived mutant mice.

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Section: Discussionmentioning

confidence: 99%

Fibroblast cell lines from young adult mice of long-lived mutant strains are resistant to multiple forms of stress

Salmon¹,

Murakami²,

Bartke³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

226

224

View full text Add to dashboard Cite

show abstract

“…Furthermore, it was recently shown that extended longevity of Drosophila in connection with artificial selection experiments is correlated with enhanced anti-oxidant defense system gene expression, accumulation of CuZn-SOD protein, and an increase in ADS enzyme activities (Arking et al, 2000). If these antioxidant mechanisms are present in bees too, the prediction would be that they are much less pronounced in foragers than in winter bees.…”

Section: Empirical Support For the Disposable Soma Theory In Honeybeesmentioning

confidence: 99%

The Regulatory Anatomy of Honeybee Lifespan

Amdam¹,

Omholt²

2002

Journal of Theoretical Biology

249

209

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“…Many genetic manipulations that extend lifespan in C. elegans (Kenyon, 2001) and Drosophila (Lin et al, 1998;Luckinbill, 1998;Parkes et al, 1998;Arking et al, 2000) enhance survival under stressful environmental conditions. To determine whether the Fig.…”

Section: Figmentioning

confidence: 99%

Delayed behavioural aging and altered mortality in Drosophilaβ integrin mutants

et al. 2003

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SummaryThe genetic basis for aging is being intensely investigated in a variety of model systems. Much of the focus in Drosophila has been on the molecular-genetic determinants of lifespan, whereas the molecular-genetic basis for age-related functional declines has been less vigorously explored. We evaluated behavioural aging and lifespan in flies harbouring loss-of-function mutations in myospheroid , the gene that encodes β β β β PS, a β β β β integrin. Integrins are adhesion molecules that regulate a number of cellular processes and developmental events. Their role in aging, however, has received limited attention. We report here that age-related declines in locomotor activity are ameliorated and that mean lifespan is increased in myospheroid mutants. The delayed functional senescence and altered mortality in myospheroid flies are independent of changes in body size, reproduction or stress resistance. Our data indicate that functional senescence and age-dependent mortality are influenced by β β β β integrins in Drosophila .

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Forward and reverse selection for longevity in Drosophila is characterized by alteration of antioxidant gene expression and oxidative damage patterns☆

Cited by 118 publications

References 36 publications

Fibroblast cell lines from young adult mice of long-lived mutant strains are resistant to multiple forms of stress

Fibroblast cell lines from young adult mice of long-lived mutant strains are resistant to multiple forms of stress

The Regulatory Anatomy of Honeybee Lifespan

Delayed behavioural aging and altered mortality in Drosophilaβ integrin mutants

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