Fouling of microporous membranes in biological applications

Le, M. S.; Gollan, K.L.

doi:10.1016/0376-7388(89)89007-6

Cited by 24 publications

(14 citation statements)

References 7 publications

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“…The sharp initial decline in permeate fluxes after an increase in TMP followed by periods of relatively constant flux is common to many membrane filtration processes (Davis, 1992;Le and Gollan, 1989;Le et al, 1984a, b;Riesmeier et al, 1989). Following a change in TMP, there was not much change in either the permeate flux or the protein transmission between the first samples taken in the initial 5 and 15 min and the samples taken after 1 h. Other studies with cell lysates also reached quasi-steady-state permeate fluxes in less than 30 min (Le and Gollan, 1989;Le et al, 1984a, b;Gabler and Ryan, 1985). As was observed in earlier studies, we found that the membrane pore size had little effect on the declines of the permeate fluxes and the protein transmissions (McDonogh et al, 1992).…”

Section: Membrane Performance Over Timementioning

confidence: 99%

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization

Bailey

Meagher

1997

Biotechnol. Bioeng.

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Crossflow membrane filtration was used to process recombinant Escherichia coli cell lysates containing protein inclusion bodies after high pressure homogenization. The number of passes through the high pressure homogenizer changed the viscosities and average particle sizes of the cell lysates. The different cell lysates were processed with a hollow fiber unit containing microfiltration membranes and a plate and frame unit with either ultrafiltration or microfiltration membranes. There were differences in permeate flux and protein transmission for the various membranes with the best performing membranes giving permeate fluxes greater than 60 L m −2 h −1 and protein transmissions greater than 90%. For a given membrane, no differences were observed between the cell lysates following homogenization with one, two, and three passes at 83 MPa. The lack of a difference between the three lysates is due to their similarities with respect to the released macromolecules and the presence of small (<0.1 µm) cell debris.

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Section: Membrane Performance Over Timementioning

confidence: 99%

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization

Bailey

Meagher

1997

Biotechnol. Bioeng.

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“…The reduced flux could be due to concentration polarization, pore plugging, fouling and secondary layer formation 23, 24. In addition, the nature of the membrane, whether hydrophilic or hydrophobic, also plays a role 25. As discussed earlier, centrifugation removed suspended impurities thus reducing the secondary layer formation on the membrane surface enabling higher flux as well as greater permeation of smaller constituents.…”

Section: Resultsmentioning

confidence: 99%

Purification of Aspergillus carbonarius polygalacturonase using polymeric membranes

Nakkeeran

Venkatesh

Subramanian

et al. 2008

J of Chemical Tech & Biotech

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BACKGROUND: Microfiltration (MF: 70-450 nm) and ultrafiltration (UF: 10-500 kDa) membranes were used to eliminate carbohydrates and other non-protein impurities from Aspergillus carbonarius culture broth containing polygalacturonase enzyme (EC 3.2.1.15) that would otherwise interfere with the purification processes and lead to enzyme loss. Further, diafiltration was attempted to improve the elimination of impurities as well as recovery of enzymes.

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“…MF has more fouling problem as compared with other membrane processes [4.13] and therefore a lot of experimental work seems necessary in this field. Fouling in CH using MF both in crossflow and deadend, has been the subject of limited number of research studies [6,[14][15][16]. Analysis of resistances in MF for beer I:1, whey E181 , and colloids [19] have been investigated.…”

Section: Introductionmentioning

confidence: 99%

Membrane fouling in microfiltration used for cell harvesting

Kaghazchi

Zokaee

Zare³

2001

J. of Therm. Sci.

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In the present study the membrane fouling in microfiltration used for cell harvesting in a deadend system has been investigated. Experimental results were analysed in terms of existing membrane filtration models and membrane resistances. The cake filtration model (CFM) and standard blocking model (SBM) have been considered in this study.Various membrane resistances were determined at different processing time, feed concentration and stirring speed. Resistances to permeation in this system include filter medium, pore blocking, adsorption, cake layer and concentration polarization.

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Fouling of microporous membranes in biological applications

Cited by 24 publications

References 7 publications

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization

Purification of Aspergillus carbonarius polygalacturonase using polymeric membranes

Membrane fouling in microfiltration used for cell harvesting

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