The Yarlung Zangbo River Basin (YZRB), situated within the Qinghai‐Tibetan Plateau, has experienced significant alterations due to global warming and vegetation greening. This region serves as a critical indicator of the interplay between vegetation growth and climatic fluctuations, as evidenced by substantial changes in spatiotemporal land surface temperature (LST) over recent decades. In this research, we assessed the components of the water and energy cycles from 1980 to 2015 utilising the variable infiltration capacity (VIC) model to generate a continuous daily LST data over a 35‐year period. Subsequently, we analysed the variations in LST and identified the influence of environmental factors on temperature changes. Notably, while greening was observed, LST exhibited an upward trend. By differentiating the effects of climatic and anthropogenic factors on LST, we found that climate was the predominant influence, accounting for a contribution rate of 70.36% from 1980 to 1995. In contrast, human activities became the primary driver of LST changes, contributing 55% after 1995. Grasslands with moderate coverage demonstrated potential cooling effects. Among the various environmental factors examined, albedo exhibited a negative and delayed impact on LST, while temperature, precipitation and evapotranspiration were positively correlated with LST, displaying relatively synchronous variations. Additionally, soil moisture and the normalised difference vegetation index (NDVI) were identified as leading contributors to positive changes in LST. This study enhances the understanding of the mechanisms influencing LST and provides essential insights for socio‐economic development in areas with sensitive ecosystem.