Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Hay, Rebecca; Gibson, Graham M.; Lee, M. P.; Padgett, Miles J.; Phillips, David B.

doi:10.1364/oe.22.018662

Cited by 12 publications

(8 citation statements)

References 21 publications

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“…Another promising approach used a spatial light modulator in reflection geometry to provide 3D information in an optical microscope ( [179], figure 17). Another simpler method uses two detectors that receive images from slightly different focal planes to synthesize 3D information (figure 18).…”

Section: D Tracking Techniquesmentioning

confidence: 99%

Advances in the microrheology of complex fluids

2016

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New developments in the microrheology of complex fluids are considered. Firstly the requirements for a simple modern particle tracking microrheology experiment are introduced, the error analysis methods associated with it and the mathematical techniques required to calculate the linear viscoelasticity. Progress in microrheology instrumentation is then described with respect to detectors, light sources, colloidal probes, magnetic tweezers, optical tweezers, diffusing wave spectroscopy, optical coherence tomography, fluorescence correlation spectroscopy, elastic- and quasi-elastic scattering techniques, 3D tracking, single molecule methods, modern microscopy methods and microfluidics. New theoretical techniques are also reviewed such as Bayesian analysis, oversampling, inversion techniques, alternative statistical tools for tracks (angular correlations, first passage probabilities, the kurtosis, motor protein step segmentation etc), issues in micro/macro rheological agreement and two particle methodologies. Applications where microrheology has begun to make some impact are also considered including semi-flexible polymers, gels, microorganism biofilms, intracellular methods, high frequency viscoelasticity, comb polymers, active motile fluids, blood clots, colloids, granular materials, polymers, liquid crystals and foods. Two large emergent areas of microrheology, non-linear microrheology and surface microrheology are also discussed.

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Section: D Tracking Techniquesmentioning

confidence: 99%

Advances in the microrheology of complex fluids

2016

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“…From a medical perspective, SM allows the visualization of microscopic surfaces magnified and dilated in all dimensions giving the observer an accurate sense of its real 3D topographical appearance (12,13,14) . Such perception allows observers to assess and precisely manipulate most specimens without the aid of specialized devices.…”

Section: Principles and Rationale For Sm Vision In Gi Researchmentioning

confidence: 99%

Stereomicroscopy and 3D-target myeloperoxidase intestinal phenotyping following a fecal flora homogenization protocol

Rodriguez-Palacios¹,

Aladyshkina²,

Cominelli³

2015

Protocol Exchange

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We recently described a unique phenotyping protocol based on stereomicroscopy (SM) to comprehensively characterize the intestinal mucosal topography in healthy and diseased mice (3D-SMAPgut). Such stereoscopic enterophenotyping approach has allowed the elucidation of novel pathophysiological mechanisms to explain three-dimensional (3D)-inflammatory structure patterns (stereoenterotypes) in the intestinal tract. Here we describe a step-wise protocol to assess the inflammatory reactivity of intestinal tissues using an improved cost-effective myeloperoxidase activity (96-well dianisidine) assay on SM-microdissected lesions harvested from intestinal specimens according to 3D-SM appearance. In addition, we emphasize the importance of controlling for gut flora effects by describing a laboratory protocol to harvest fecal and soiled bedding material from experimental mouse groups; the goal of this ('inter-subject Pre-experimental Fecal flora Homogenization 'IsPreFeH') is to expose all investigational subjects to a composite of fecal material prior to experimentation as an alternative to cohousing which may not be always feasible with males and in all strains. Together, this methodology has enabled documenting the relevance of SM profiling of inflammatory 3D-structures in the gut while controlling for the cage effects of gut flora variability.

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“…For example, the first necessary step for statistically analyzing the trajectory of a trapped particle is to track the particle position accurately. This is often achieved by acquiring a video 6,7 , stereomicroscopy 8 , V-shaped micro-mirrors 9 of the particle, or other visualization techniques including position sensitive detection 10 and quadrant photodiode detection 11 , which monitors via detecting the backscattering signal or transmitting signal. In fact, these experimental techniques are limited by sampling rate and in 3D imaging applications.…”

Section: Introductionmentioning

confidence: 99%

3D dynamic motion of a dielectric micro-sphere within optical tweezers

Liu¹,

Zheng²,

Xiong³

et al. 2021

OEA

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Known as laser trapping, optical tweezers, with nanometer accuracy and pico-newton precision, plays a pivotal role in single bio-molecule measurements and controllable motions of micro-machines. In order to advance the flourishing applications for those achievements, it is necessary to make clear the three-dimensional dynamic process of micro-particles stepping into an optical field. In this paper, we utilize the ray optics method to calculate the optical force and optical torque of a micro-sphere in optical tweezers. With the influence of viscosity force and torque taken into account, we numerically solve and analyze the dynamic process of a dielectric micro-sphere in optical tweezers on the basis of Newton mechanical equations under various conditions of initial positions and velocity vectors of the particle. The particle trajectory over time can demonstrate whether the particle can be successfully trapped into the optical tweezers center and reveal the subtle details of this trapping process. Even in a simple pair of optical tweezers, the dielectric micro-sphere exhibits abundant phases of mechanical motions including acceleration, deceleration, and turning. These studies will be of great help to understand the particle-laser trap interaction in various situations and promote exciting possibilities for exploring novel ways to control the mechanical dynamics of microscale particles.

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Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Cited by 12 publications

References 21 publications

Advances in the microrheology of complex fluids

Advances in the microrheology of complex fluids

Stereomicroscopy and 3D-target myeloperoxidase intestinal phenotyping following a fecal flora homogenization protocol

3D dynamic motion of a dielectric micro-sphere within optical tweezers

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