Four new HLA class I alleles in Cauacasoids^*

Abstract

Four new HLA classical class I alleles in the three loci are described in Caucasian individuals. A*3012 was first suspected by an abnormal serologic pattern that would be explained by the single amino acid substitution at the A30-specific Ser17. B*270505 differs from B*270502 in a silent substitution at an up to now constant position in the B locus. B*3541 encodes for a new Cys at position 118 that has not been encountered in neither human nor primate alleles. Cw*0716 seems to be originated by a large-scale interallelic recombination event between Cw*0701/*0706/*0718 and Cw*020202, giving rise to a new antigen-binding cleft conformation.

Identification of the very uncommon allele HLA‐Cw*0716 in a Caucasian renal transplant recipient: extension of the exon 4 sequence

“…Nucleotide sequence alignments with HLA‐C alleles from the IMGT/HLA Sequence Database (5) showed that the exon 4 sequence of the Cw*0716 is identical to the Cw*070101 allele (Figure 1). This result allowed us to confirm the hypothesis of the generation of Cw*0716 allele by an interallelic recombination event between Cw*0701/0706/0718 and Cw*020202 allele, because of the identity with the former for the sequence from the end of intron 1 to the beginning of intron 3, except for a fragment located at the 3′ end of exon 2 to the first half of the intron 2, which matches completely with the Cw*020202 allele (1).…”

“…In particular, the Cw*0716 allele was only identified in a Caucasian bone marrow donor from the Netherlands (1) and for the first time in our sample (E770) from Italy.…”

A novel HLA‐Cw*07 variant, HLA‐Cw*0760, identified by sequence‐based typing in a bone marrow donor

“…It has been described that HLA‐Cw*0716 was originated by a large‐scale interallelic recombination event between Cw*0701/*0706/*0718 and Cw*020202 (1, 2). To know if the novel Cw*0760 arise from Cw*0716, we compared the intron 2 sequences.…”