Four New Species in Lipomyces

Walt, J. P. van der; Smith, Maudy Th.; Rœijmans, H. J.

doi:10.1016/s0723-2020(99)80070-8

Cited by 6 publications

(11 citation statements)

References 10 publications

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“…As a result, four distinct clusters of reassociation strains of L. starkeyi were identified, i.e., L. starkeyi sensu stricto and L. starkeyi clusters a, b, and c. Van der Walt et al (1997) examined the possibility of genetic exchange by prototrophic selection techniques, and suggested that L. starkeyi cluster a should be designated as a distinct species called L. mesembrius. Lipomyces starkeyi clusters b and c were treated as distinct species, L. doorenjongii and L. kockii, respectively (Van der Walt et al 1999). Phylogenetic analysis of partial 5.8S rRNA and internal transcribed spacer (ITS) 2 sequences showed that L. starkeyi s.s. and its associated clusters b and c, and L. mesembrius formed a monophyletic group with L. tetrasporus (Gouliamova et al 1998).…”

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confidence: 99%

Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi

Oguri

Masaki

Naganuma

et al. 2011

Antonie van Leeuwenhoek

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Lipomyces starkeyi is an oleaginous yeast, and has been classified in four distinct groups, i.e., sensu stricto and custers α, β, and γ. Recently, L. starkeyi clusters α, β, and γ were recognized independent species, Lipomyces mesembrius, Lipomyces doorenjongii, and Lipomyces kockii, respectively. In this study, we investigated phylogenetic relationships within L. starkeyi, including 18 Japanese wild strains, and its related species, based on internal transcribed spacer sequences and evaluated biochemical characters which reflected the phylogenetic tree. Phylogenetic analysis showed that most of Japanese wild strains formed one clade and this clade is more closely related to L. starkeyi s.s. clade including one Japanese wild strain than other clades. Only three Japanese wild strains were genetically distinct from L. starkeyi. Lipomyces mesembrius and L. doorenjongii shared one clade, while L. kockii was genetically distinct from the other three species. Strains in L. starkeyi s.s. clade converted six sugars, D-glucose, D-xylose, L-arabinose, D-galactose, D-mannose, and D-cellobiose to produce high total lipid yields. The Japanese wild strains in subclades B, C, and D converted D-glucose, D-galactose, and D-mannose to produce high total lipid yields. Lipomyces mesembrius was divided into two subclades. Lipomyces mesembrius CBS 7737 converted D-xylose, L-arabinose, D-galactose, and D-cellobiose, while the other L. mesembrius strains did not. Lipomyces doorenjongii converted all the sugars except D-cellobiose. In comparison to L. starkeyi, L. mesembrius, and L. doorenjongii, L. kockii produced higher total lipid yields from D-glucose, D-galactose, and D-mannose. The type of sugar converted depended on the subclade classification elucidated in this study.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi

Oguri

Masaki

Naganuma

et al. 2011

Antonie van Leeuwenhoek

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“…Three of the groups were composed of subgroups that showed 47–69% DNA relatedness. Van der Walt et al (1997, 1999) suggested that the six well‐isolated groups, as well as the subgroups, represented individual species, resulting in the description of five new species and the elevation of a subspecies to species status. The 11 species accepted in these studies were the following: Lipomyces doorenjongii, L .…”

Section: Introductionmentioning

confidence: 99%

Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer ofZygozymaspecies toLipomycesandBabjevia anomalatoDipodascopsis

Kurtzman

Albertyn

Basehoar-Powers

2007

FEMS Yeast Research

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Phylogenetic relationships among species assigned to genera of the family Lipomycetaceae were determined from analysis of the nearly entire large, subunit rRNA gene, the small subunit rRNA gene, mitochondrial small subunit rRNA gene and the translation elongation factor-1alpha gene. Monophyly of the Lipomycetaceae was strongly supported, and currently described species appear genetically unique. The multigene analysis provided no support for maintaining the genera Kawasakia, Smithiozyma, Waltomyces or Zygozyma, and it is proposed that species in these genera be assigned to the genus Lipomyces. The monotypic genus Babjevia is a member of the Dipodascopsis clade and it is proposed to reassign Babjevia anomala to Dipodascopsis. The proposed changes will result in the Lipomycetaceae having two ascosporic genera, Lipomyces and Dipodascopsis, and the anamorphic genus Myxozyma.

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“…For example, taxonomic criteria such as the sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene, ascospore morphology and assimilation profiles could not clearly indicate differences between these species (Smith & Kurtzman, 2011). However, most Lipomyces species, including L. yarrowii, L. yamadae, L. spencermartinsiae, L. kockii, L. doorenjongii and L. mesembrius, were shown to be independent species according to the results of DNA hybridization (Smith et al, 1995;van der Walt et al, 1997van der Walt et al, , 1999. Kurtzman et al (2007) conducted a reclassification of the family Lipomycetaceae by using four genes, namely the small-subunit (SSU) rRNA gene, the mitochondrial small-subunit (MtSSU) rRNA gene, the LSU rRNA gene and the translation elongation factor 1 alpha .…”

Section: Introductionmentioning

confidence: 99%

Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae

Yamazaki

Kawasaki

2014

International Journal of Systematic and Evolutionary Microbiology

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Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae Atsushi Yamazaki and Hiroko KawasakiCorrespondence Atsushi Yamazaki yamazaki-atsushi@nite.go.jp NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, JapanWe isolated two strains of a novel Lipomyces species from soil collected in Chichibu forest, Saitama prefecture, Japan. Based on their morphological and biochemical characteristics, along with multilocus sequence typing using the D1/D2 domain of the large-subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region and the translation elongation factor 1 alpha gene (EF-1a), the two strains were shown to represent a novel species of the genus Lipomyces, described as Lipomyces chichibuensis sp. INTRODUCTIONSpecies belonging to the oleaginous yeast genus Lipomyces can accumulate lipids. This ability is expected to have wide applications in the industrial production of lipids, including biofuels (Starkey, 1946;Naganuma et al., 1985Naganuma et al., , 1986Zhao et al., 2008;Meng et al., 2009;Oguri et al., 2012). At the time of writing, the genus Lipomyces contains 14 species, which are only slightly different from each other, especially the 12 species , 1997, 1999). Kurtzman et al. (2007) conducted a reclassification of the family Lipomycetaceae by using four genes, namely the small-subunit (SSU) rRNA gene, the mitochondrial small-subunit (MtSSU) rRNA gene, the LSU rRNA gene and the translation elongation factor 1 alpha . This study demonstrated that each Lipomyces species was clearly distinguishable by the sequences of these four genes.The most recently described Lipomyces species, L. orientalis, was isolated in 2006 (Thanh, 2006). Since then, no novel Lipomyces species has been described. In this study, we isolated a novel species of the genus Lipomyces, designated Lipomyces chichibuensis sp. nov., in Chichibu forest, Saitama prefecture, Japan, and characterized it in comparison with 12 related species of the genus Lipomyces based on conventional methods and multigene phylogenetic analysis using three sequences, the LSU rRNA gene, the ITS region and EF-1a.Because we found some discrepancies among the type strains of two species, L. kononenkoae and L. spencermartinsiae (Niewdrop et al., 1974; van der Walt et al., 1997), maintained in the Centraalbureau voor Schimmelcultures (CBS), the ARS Culture Collection (NRRL) and the NITE Biological Resource Center (NBRC) culture collections, we also identified the correct type strains of these species by their physiological characteristics and DNA-DNA relatedness.Abbreviations: ITS, internal transcribed spacer; LSU, large-subunit; ML, maximum-likelihood; MP, maximum-parsimony; NJ, neighbour-joining; SSU, small-subunit.The GenBank/EMBL/DDBJ accession numbers for the 26S rRNA gene D1/D2, ITS and EF-1a sequences of strain CB08-2 T are AB828721, AB828723 and AB828725.A supplementary ...

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Four New Species in Lipomyces

Cited by 6 publications

References 10 publications

Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi

Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi

Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer ofZygozymaspecies toLipomycesandBabjevia anomalatoDipodascopsis

Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae

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