Four or More Species of Cladosporium Sympatrically Colonize Phragmites australis

Wirsel, Stefan G. R.; Runge-Froböse, Christiane; Ahrén, Dag; Kemen, Eric; Oliver, Richard P.; Mendgen, Kurt

doi:10.1006/fgbi.2001.1314

Cited by 39 publications

(46 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such cultivation might require procedures that are currently not available for mycology and thus need to be developed. The first outcome of this study is that the mycoflora associated with mature, healthy reeds is much more diverse than previous cultivation-based approaches that covered the same sampling sites had indicated (9,41,42). This was true for all vegetative organs analyzed.…”

Section: Discussionmentioning

confidence: 68%

“…Sequence analysis of the internal transcribed spacer (ITS) region, which is part of the rRNA gene cluster, indicated that these isolates belonged to at least 17 genera. This previous work and follow-up studies of two distinct genera, Cladosporium and Stagonospora, revealed that at least 30 fungal species colonized healthy P. australis (9,41,42). Our investigations were complemented by a PCRbased study targeting most of the currently known taxa in the Glomeromycota (40), which are the arbuscular mycorrhiza fungi found in the roots of many land plants.…”

mentioning

confidence: 66%

“…A threshold of 97% similarity was used as an approximation to differentiate the sequences at a level close to the species level, by analogy to the practice used to distinguish bacterial species by their 18S rRNA sequences, since it was reported that in the Basidiomycota the level of intraspecies variation commonly ranges from 0 to 3% (43). Using the 97% threshold, we identified five sequences that were the best database matches with our previous isolates from reeds that were obtained by conventional cultivation (9,41,42). The two most common OTUs found in this study, Ms9Mb4 and Ms4Mb4a, thus corresponded to a Cladosporium sp.…”

Section: Resultsmentioning

confidence: 99%

“…The pools were homogenized with a mixer mill (MM 300; QIAGEN GmbH, Hilden, Germany), and 100-mg aliquots were used for DNA extraction using a DNeasy plant mini kit (QIAGEN). DNA quality was controlled as described previously (42).…”

Section: Methodsmentioning

confidence: 99%

“…DNA sequences were generated, assembled, aligned, and edited as described previously (42). We used two approaches to identify putative chimeras.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Only a Few Fungal Species Dominate Highly Diverse Mycofloras Associated with the Common Reed

Neubert

Mendgen

Brinkmann

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

Plants are naturally colonized by many fungal species that produce effects ranging from beneficial to pathogenic. However, how many of these fungi are linked with a single host plant has not been determined. Furthermore, the composition of plant-associated fungal communities has not been rigorously determined. We investigated these essential issues by employing the perennial wetland reed Phragmites australis as a model. DNA extracted from roots, rhizomes, stems, and leaves was used for amplification and cloning of internal transcribed spacer rRNA gene fragments originating from reed-associated fungi. A total of 1,991 clones from 15 clone libraries were differentiated by restriction fragment length polymorphism analyses into 345 operational taxonomical units (OTUs). Nonparametric estimators for total richness (Chao1 and ACE) and also a parametric log normal model predicted a total of about 750 OTUs if the libraries were infinite. Sixty-two percent of the OTUs sequenced were novel at a threshold of 3%. Several of these OTUs represented undocumented fungal species, which also included higher taxonomic levels. In spite of the high diversity of the OTUs, the mycofloras of vegetative organs were dominated by just a few typical fungi, which suggested that competition and niche differentiation influence the composition of plant-associated fungal communities. This suggestion was independently supported by the results of nested PCR assays specifically monitoring two OTUs over 3 years, which revealed significant preferences for host habitat and host organ.

show abstract

Section: Discussionmentioning

confidence: 68%

mentioning

confidence: 66%