Fourier analysis of cell motility: correlation of motility with metastatic potential.

Partin, Alan W.; Schoeniger, Joseph S.; Mohler, James L.; Coffey, Donald S.

doi:10.1073/pnas.86.4.1254

Cited by 172 publications

(99 citation statements)

References 19 publications

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“…Ruffles that project inside these contours (1,2,5) will not be taken into account. We assume that the method of Partin et al (17) is not suited for the assessment of ruffling in cells enclosed by other cells such as in epithelial cell islands and in confluent monolayers. Recently Tatsuka et al (22) described a method for quantification of "momentary alterations of cell shape" reflecting ruffling, pseudopodal activity, and fast cell translocation, using automatic image analysis on Allen video-enhanced contrast-differential interference contrast images.…”

Section: Discussionmentioning

confidence: 99%

“…Various aspects of cell motility (see Methods for terminology used in this paper) can be assessed in vitro. Several methods have been described to quantify translocation (7,15,23), directional migration including chemokinesis and chemotaxis (13,251, as well as shape change (17,24). Quantification of fast plasma membrane movements involved in ruffling, blebbing, formation or movement of cytopodia, and fast shape change or fast translocation has received less attention.…”

mentioning

confidence: 99%

“…Quantification of fast plasma membrane movements involved in ruffling, blebbing, formation or movement of cytopodia, and fast shape change or fast translocation has received less attention. Partin et al (17) have described an elaborate Fourier analysis for quantification of the various aspects of cell motility, including ruffling. Recently Tatsuka et al (22) described a method for quantification of "momentary alterations of cell shape" reflecting ruff ling, pseudopodal activity, and fast cell translocation, using automatic image analysis on Allen video-enhanced contrast-differential interference contrast images.…”

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confidence: 99%

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Simple method for quantification of fast plasma membrane movements

1992

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We present a method for the quantification of the fast plasma membrane movements that are involved in ruffling, blebbing, fast shape change, and fast translocation. The method is based on the Kontron Vidas image analysis computer program. Video images from cells viewed through an inverted microscope were transmitted to the computer. The procedure was as follows: 4 consecutive video images were averaged (imagel); 28 s later a second set of 4 video images was averaged (image 2); image 2 was subtracted from image 1 and the grey level of each pixel of the resulting image was increased with 128 grey level units, resulting in the subtraction image, showing a uniform grey background speckled with brighter and darker spots corresponding to areas of movement. These spots were discriminated and turned into white objects against a black background. Interactive editing was used to delete artefacts that resulted from floating debris. The total area of the discriminated objects was measured, and the parameter motile area in pm2 per cell was calculated. We have applied our method to the study of motility induced in epithelial cell lines by the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate and by epidermal growth factor.Key terms: Cell motility, cell membrane, cell-surface, ruffling, blebbing, cellshape, translocation, image analysis, TPA, EGF Direct analysis of cell motility in vivo is problematic because of the lack of transparency of three-dimensional living tissues. There is evidence indicating that the study of cell motility on a flat surface in vitro is relevant for the situation in vivo because cells have an inherent motility program (11,12,21). Various aspects of cell motility (see Methods for terminology used in this paper) can be assessed in vitro. Several methods have been described to quantify translocation (7,15,23), directional migration including chemokinesis and chemotaxis (13,251, as well as shape change (17,24). Quantification of fast plasma membrane movements involved in ruffling, blebbing, formation or movement of cytopodia, and fast shape change or fast translocation has received less attention. Partin et al. (17) have described an elaborate Fourier analysis for quantification of the various aspects of cell motility, including ruffling. Recently Tatsuka et al. (22) described a method for quantification of "momentary alterations of cell shape" reflecting ruff ling, pseudopodal activity, and fast cell translocation, using automatic image analysis on Allen video-enhanced contrast-differential interference contrast images. Here, we report on a simple and rapid method for quantification of fast plasma membrane movements. It is entirely based on the Vidas automatic grey level image analysis program that is commercially available from Kontron (Eching, West Germany). We have tested our method on cell cultures differing in their type or intensity of motility as estimated from time-lapse video films. As an application of our method we analysed the effect of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acet...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Simple method for quantification of fast plasma membrane movements

1992

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“…Tumour cell migration and invasion are critical steps in metastasis [4][5][6][7][8][9][10] . Migration provides tumour cells the ability to leave the primary tumour bed (local invasion), enter into blood vessels, then exit the circulation and infiltrate the distant tissues/ organs.…”

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Targeted inhibition of fascin function blocks tumour invasion and metastatic colonization

et al. 2015

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One of the key steps during tumour metastasis is tumour cell migration and invasion, which require actin cytoskeletal reorganization. Among the critical actin cytoskeletal protrusion structures are the filopodia, which act like cell sensory organs to communicate with the extracellular microenvironment and participate in fundamental cell functions such as cell adhesion, spreading and migration in the three-dimensional environment. Fascin is the main actin-bundling protein in filopodia. Using high-throughput screening, here we identify and characterize small molecules that inhibit the actin-bundling activity of fascin. Focusing on one such inhibitor, we demonstrate that it specifically blocks filopodial formation, tumour cell migration and invasion in vitro, and metastasis in vivo. Hence, target-specific anti-fascin agents have a therapeutic potential for cancer treatment.

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“…Although the mechanism of local invasion is still largely unknown, one crucial requirement is acquisition and enhancement of cell motility. 4 Various molecular components of the cytoskeleton, cell adhesion, and signaling systems seem to be involved in the regulation of cell motility. [5][6][7][8] Actinin-1 is an isoform of non-muscular a-actinin preferentially localized on the inner surface of cells, being a component of focal adhesion plaques and cell-to-cell adherence junctions.…”

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Actinin-4 expression in ovarian cancer: a novel prognostic indicator independent of clinical stage and histological type

Yamamoto

Tsuda

Honda³

et al. 2007

Modern Pathology

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Actinin-4 is an isoform of non-muscular a-actinin and actin-bundling protein. By enhancing cell motility, actinin-4 shows different biological properties from another isoform of non-muscular actinin 'actinin-1' and variable clinicopathological implications of actinin-4 have been demonstrated in some human malignancies such as breast cancers, lung cancers, and colorectal cancers. We herein described the clinicopathological and prognostic significance of actinin-4 expression in ovarian cancers. Actinin-4 expression was analyzed immunohistochemically in 265 primary ovarian carcinomas: 116 serous, 71 clear cell, 43 endometrioid, and 35 mucinous adenocarcinomas. With reference to endothelial immunoreactivity, cytoplasmic expression of actinin-4 was classified as either low (including negative) or high. Then, various parameters such as patients' characteristics, histopathological findings including E-cadherin and b-catenin immunoreactivity, and clinical outcome, were compared between groups showing differences in the intensity or intracellular distribution of actinin-4 immunoreactivity. High expression of actinin-4 was demonstrated in 137 (57%) cases and was associated with serous histology (P ¼ 0.0075), high histological grade (Po0.0001), an advanced disease stage (P ¼ 0.036), a high degree of residual disease after initial surgery (P ¼ 0.0047), poor patient outcome (5-year survival: 52.4% in the high-expression group vs 71.9% in the low expression group, P ¼ 0.0043 by log-rank test), and also with reduced E-cadherin and preserved b-catenin expressions (P ¼ 0.0097 and 0.017, respectively). Nuclear immunoreactivity for actinin-4 was detected in 20 (7.5%) cases and was associated with low histological grade (P ¼ 0.0079) but not with other variables. Multivariate analysis showed that high actinin-4 expression was an independent prognostic factor for overall survival, as well as a high degree of residual disease and clear-cell histology. Accumulation of actinin-4 in the cytoplasm may be related to a higher propensity for tumor invasiveness and metastasis, probably by enhancing cell motility, and could be a novel prognostic indicator for patients with ovarian carcinomas.

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Fourier analysis of cell motility: correlation of motility with metastatic potential.

Cited by 172 publications

References 19 publications

Simple method for quantification of fast plasma membrane movements

Simple method for quantification of fast plasma membrane movements

Targeted inhibition of fascin function blocks tumour invasion and metastatic colonization

Actinin-4 expression in ovarian cancer: a novel prognostic indicator independent of clinical stage and histological type

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