Fourier transform infrared conformational investigation of type I collagen aged by in vitro induced dehydration and non-enzymatic glycation treatments

Bridelli, Maria Grazia; Stani, Chiaramaria; Bedotti, Roberta

doi:10.4081/jbr.2017.6254

Cited by 16 publications

(13 citation statements)

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“…Moreover, it has also been reported that aging affects the amide I to amide II ratio, as seen in Fig. 2c, lowering the signal of amide II and increasing of the signals in the 1100-1000 cm −1 spectral region, as a consequence of the formation of AGEs (Advanced Glycation Endproducts) 31 . Clearly, aging and signal artifact due to the sampling geometry affected the spectral quality and peak position.…”

Section: Resultsmentioning

confidence: 64%

Surface analysis of an eagle talon from Krapina

Radovčić

Birarda

Sršen

et al. 2020

Sci Rep

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the Krapina white-tailed eagle talons represent a kind of jewelry worn by Krapina neandertals some 130,000 years ago. New inspection of one Krapina talon (386.1) revealed a fiber, sealed by a thin silicate coating, adhering to the surface within a wide cut mark, as well as concentrated traces of occasional spots of red and yellow pigment and some black stains. We analyzed the fiber and small portions of pigmented areas by non-invasive, infrared synchrotron beam. Different areas were targeted, revealing the protein nature of the fiber, identified as of animal origin. Targeted areas revealed intra-and interstrand aggregation indicating the fiber to be collagen losing its original triple α-helix conformation, further confirming the diagenetic decay of the original collagen structure and the antiquity of the fiber. It is possible that the fiber is a remnant of the leather or sinew string binding the talons together. Spectroscopic analysis of the pigments in two isolated areas confirmed two types of ochre and that the dark spots are charcoal remnants. Applying novel non-invasive technologies provides new possibilities to further test the hypothesis of using prehistoric objects for symbolic purposes. Eight white-tailed eagle (Haliaëtus albicilla) talons and one foot phalanx were found at the Krapina Neandertal site, excavated between 1899-1905 1-3. The deposits are dated to 130,000 BP by ESR and uranium series 4. Only Neandertals were using the site, evidenced by Mousterian stone tools found in all the Krapina layers, as well as Neandertal remains found in all except the lowest layer. Yet, even here Mousterian stone tools were found in addition to faunal remains with anthropogenic cut marks 5-7. Deriving from the uppermost level, the talons are thought to be one of the earliest examples of Neandertal ornaments 8. The fact that eight talons were found in the same context together with an additional phalanx, and the fact that all of them contain evidence of anthropogenic modifications such as cut marks, nicks from the medial and lateral aspects of the plantar surface, and several heavily polished areas suggest that these were an assemblage worn as jewelry. Based on duplication of right talon 2 the talons belong to at least three different birds, indicating that acquiring them was intentional and not a fortuitous capture of a single eagle 8. This raptor species is a top diurnal avian predator with two-meter wingspan, not commonly encountered in the environment 9 and not an easy prey to acquire 10,11. So far, raptor talons and phalanges have been found at various European Neandertal sites, together with the remains of Mousterian and Châtelperronian techno-cultures 12-17. All these sites provide evidence of never more than a single talon in the same archaeological level. Only Krapina has multiple talons found in one level with multiple signs of manipulation strongly suggesting they were combined into a personal ornament. They are indeed unique in the European fossil record and, even in the Upper Paleolithic or Mesolithic pe...

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Section: Resultsmentioning

confidence: 64%

Surface analysis of an eagle talon from Krapina

Radovčić

Birarda

Sršen

et al. 2020

Sci Rep

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“…Similar extracellular matrix compositional changes (higher AGEs and cross-linking) and lower bound water were observed alongside lower fracture resistance in aged (24 mo) male Fischer F344 rats ( Uppuganti et al, 2016 ). As a result of these and other works ( Bridelli et al, 2017 ; Jazini et al, 2012 ), it is hypothesized that loss of bound water and increased glycation are related and consequential steps during aging. While it is not entirely clear, evidence supports a loss of bound water may initiate glycation events ( Bridelli et al, 2017 ) while increased cross-linking may physically displace existing or prevent new bound water ( Kopp et al, 1989 ).…”

Section: Bone Water With Age and Diseasementioning

confidence: 83%

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

2022

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Water constitutes roughly a quarter of the cortical bone by volume yet can greatly influence mechanical properties and tissue quality. There is a growing appreciation for how water can dynamically change due to age, disease, and treatment. A key emerging area related to bone mechanical and tissue properties lies in differentiating the role of water in its four different compartments, including free/pore water, water loosely bound at the collagen/mineral interfaces, water tightly bound within collagen triple helices, and structural water within the mineral. This review summarizes our current knowledge of bone water across the four functional compartments and discusses how alterations in each compartment relate to mechanical changes. It provides an overview on the advent of- and improvements to- imaging and spectroscopic techniques able to probe nano-and molecular scales of bone water. These technical advances have led to an emerging understanding of how bone water changes in various conditions, of which aging, chronic kidney disease, diabetes, osteoporosis, and osteogenesis imperfecta are reviewed. Finally, it summarizes work focused on therapeutically targeting water to improve mechanical properties.

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“…Indeed, glycated proteins showed higher absorption near the peak located at 3400 cm −1 . Previous studies also identified the presence of a shoulder near the peak at 3400 cm −1 when glycation occurred (Bridelli et al ., 2017). Moreover, it was pointed out that the higher the shoulder values, the higher the glycation rate (Otero de Joshi et al ., 2003).…”

Section: Resultsmentioning

confidence: 99%

Glycation of soy protein isolate with two ketoses: d‐Allulose and fructose

Tas

Ertugrul

Mazı

2021

Int J of Food Sci Tech

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Summary Modification of food proteins to have improved functional properties is of great importance. In this study, modification of soy protein isolate (SPI) was achieved through glycation. SPI was glycated in a spray dryer (SD) and an incubator followed by freeze drying (FD). d‐Allulose, an important rare sugar, was used in SPI glycation as the carbohydrate source, and results were compared with fructose. In addition to the sugar type, two different SPI powder: sugar ratios (1:1 and 5:1) were investigated. For the glycated samples, emulsification activity, free amino groups, protein solubility, Fourier‐transform infrared spectroscopy analysis, antioxidant activity experiments and time‐domain NMR relaxometry measurements for hydration were conducted. According to the results, the solubility of SPI that is limited in native form has shown a significant improvement after glycation through both FD and SD methods. Besides, glycation through the FD method was found to be more favourable due to its milder conditions than the SD method. Considering the physicochemical properties, the best combination for the highest glycation degree was found to be the samples prepared at the 1:1 ratio with d‐Allulose in the FD method. Overall, it was concluded that glycation of SPI enhanced its functional properties such as antioxidant and emulsification activities.

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Fourier transform infrared conformational investigation of type I collagen aged by in vitro induced dehydration and non-enzymatic glycation treatments

Cited by 16 publications

References 0 publications

Surface analysis of an eagle talon from Krapina

Surface analysis of an eagle talon from Krapina

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

Glycation of soy protein isolate with two ketoses: d‐Allulose and fructose

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